ENSG00000135655

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000280377 ENSG00000135655 HEK293_OSMI2_2hA HEK293_TMG_2hB USP15 protein_coding protein_coding 18.47316 4.029131 29.51412 0.6613224 1.488401 2.869777 1.2433452 0.3539947 1.9220967 0.07408687 0.3032892 2.4081780 0.07217917 0.0863000 0.06560000 -0.02070000 6.354885e-01 6.96757e-12 FALSE TRUE
ENST00000312635 ENSG00000135655 HEK293_OSMI2_2hA HEK293_TMG_2hB USP15 protein_coding protein_coding 18.47316 4.029131 29.51412 0.6613224 1.488401 2.869777 2.3248296 2.3590063 3.8086357 0.13164233 0.3235529 0.6887753 0.27572083 0.6132667 0.12856667 -0.48470000 2.292657e-07 6.96757e-12 FALSE TRUE
ENST00000353364 ENSG00000135655 HEK293_OSMI2_2hA HEK293_TMG_2hB USP15 protein_coding protein_coding 18.47316 4.029131 29.51412 0.6613224 1.488401 2.869777 0.6481838 0.1277966 0.9583948 0.12779662 0.2422973 2.8130548 0.05006250 0.0472000 0.03246667 -0.01473333 7.042541e-01 6.96757e-12 FALSE TRUE
ENST00000549415 ENSG00000135655 HEK293_OSMI2_2hA HEK293_TMG_2hB USP15 protein_coding protein_coding 18.47316 4.029131 29.51412 0.6613224 1.488401 2.869777 2.6894193 0.5449860 4.1515863 0.31211259 0.7658908 2.9066101 0.11603333 0.1157667 0.13893333 0.02316667 8.055438e-01 6.96757e-12 FALSE TRUE
ENST00000550632 ENSG00000135655 HEK293_OSMI2_2hA HEK293_TMG_2hB USP15 protein_coding processed_transcript 18.47316 4.029131 29.51412 0.6613224 1.488401 2.869777 0.5590722 0.0000000 2.1215778 0.00000000 1.0966421 7.7357779 0.02845833 0.0000000 0.07566667 0.07566667 4.361388e-01 6.96757e-12 FALSE TRUE
MSTRG.7547.3 ENSG00000135655 HEK293_OSMI2_2hA HEK293_TMG_2hB USP15 protein_coding   18.47316 4.029131 29.51412 0.6613224 1.488401 2.869777 5.8996631 0.5340706 8.6225295 0.27517826 0.3395409 3.9879175 0.26218333 0.1141333 0.29270000 0.17856667 3.573607e-01 6.96757e-12 TRUE TRUE
MSTRG.7547.6 ENSG00000135655 HEK293_OSMI2_2hA HEK293_TMG_2hB USP15 protein_coding   18.47316 4.029131 29.51412 0.6613224 1.488401 2.869777 4.3474396 0.0000000 6.7864649 0.00000000 1.4197941 9.4086407 0.14297500 0.0000000 0.22683333 0.22683333 6.967570e-12 6.96757e-12 FALSE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000135655 E001 0.1472490 0.0436959417 1.000000e+00   12 62260338 62260355 18 + 0.055 0.000 -9.051
ENSG00000135655 E002 0.1472490 0.0436959417 1.000000e+00   12 62260356 62260358 3 + 0.055 0.000 -9.033
ENSG00000135655 E003 0.1472490 0.0436959417 1.000000e+00   12 62260359 62260363 5 + 0.055 0.000 -9.033
ENSG00000135655 E004 1.4048617 0.0096601566 5.832485e-01 7.073923e-01 12 62260364 62260372 9 + 0.288 0.411 0.750
ENSG00000135655 E005 7.3962552 0.0024609400 5.515283e-01 6.809749e-01 12 62260373 62260396 24 + 0.830 0.759 -0.284
ENSG00000135655 E006 7.5435042 0.0030621318 5.147940e-01 6.495164e-01 12 62260397 62260398 2 + 0.839 0.759 -0.318
ENSG00000135655 E007 11.2640322 0.0015330616 4.240831e-01 5.670855e-01 12 62260399 62260403 5 + 0.994 0.909 -0.318
ENSG00000135655 E008 11.8552184 0.0014937172 3.265784e-01 4.700065e-01 12 62260404 62260406 3 + 1.017 0.909 -0.402
ENSG00000135655 E009 12.1539828 0.0013797655 2.845763e-01 4.248212e-01 12 62260407 62260407 1 + 1.028 0.909 -0.443
ENSG00000135655 E010 32.8378906 0.0134892640 2.390083e-02 5.960091e-02 12 62260408 62260427 20 + 1.442 1.224 -0.760
ENSG00000135655 E011 67.3545536 0.0030767551 4.991022e-03 1.590389e-02 12 62260428 62260503 76 + 1.738 1.572 -0.563
ENSG00000135655 E012 0.0000000       12 62278628 62278714 87 +      
ENSG00000135655 E013 104.1242992 0.0010377795 8.998975e-04 3.613492e-03 12 62294179 62294306 128 + 1.921 1.774 -0.495
ENSG00000135655 E014 0.0000000       12 62294307 62294640 334 +      
ENSG00000135655 E015 105.5690645 0.0003219083 7.785798e-05 4.179406e-04 12 62302790 62302920 131 + 1.931 1.756 -0.588
ENSG00000135655 E016 2.0746268 0.0095377852 6.328819e-02 1.321870e-01 12 62302921 62303586 666 + 0.459 0.000 -12.840
ENSG00000135655 E017 1.4017412 0.0096049705 7.302008e-01 8.222363e-01 12 62304673 62304896 224 + 0.317 0.253 -0.442
ENSG00000135655 E018 2.2457220 0.0071186554 3.133967e-01 4.559857e-01 12 62305595 62306058 464 + 0.459 0.253 -1.250
ENSG00000135655 E019 0.1472490 0.0436959417 1.000000e+00   12 62312286 62312309 24 + 0.055 0.000 -9.033
ENSG00000135655 E020 113.7048185 0.0005929524 4.859290e-04 2.106393e-03 12 62314790 62314916 127 + 1.958 1.813 -0.488
ENSG00000135655 E021 0.1472490 0.0436959417 1.000000e+00   12 62314917 62315073 157 + 0.055 0.000 -9.033
ENSG00000135655 E022 0.0000000       12 62321461 62321463 3 +      
ENSG00000135655 E023 96.2655426 0.0020549071 6.478266e-01 7.594416e-01 12 62321464 62321609 146 + 1.864 1.863 -0.004
ENSG00000135655 E024 62.1673907 0.0012432572 1.971142e-01 3.226235e-01 12 62325872 62325933 62 + 1.658 1.757 0.334
ENSG00000135655 E025 0.3634088 0.3953763758 2.943432e-02   12 62327661 62327781 121 + 0.000 0.413 14.883
ENSG00000135655 E026 0.0000000       12 62328293 62328405 113 +      
ENSG00000135655 E027 123.1447929 0.0003826537 4.755006e-83 9.791539e-80 12 62335166 62336690 1525 + 1.759 2.428 2.241
ENSG00000135655 E028 23.5294781 0.0009299638 4.620088e-03 1.487604e-02 12 62349221 62349307 87 + 1.308 1.020 -1.027
ENSG00000135655 E029 0.0000000       12 62355011 62355074 64 +      
ENSG00000135655 E030 52.8639290 0.0004408966 5.753076e-08 6.117895e-07 12 62355331 62355427 97 + 1.658 1.245 -1.425
ENSG00000135655 E031 45.9318440 0.0004940756 7.342413e-06 5.029461e-05 12 62355428 62355475 48 + 1.594 1.245 -1.205
ENSG00000135655 E032 1.9595293 0.0071408461 4.155756e-01 5.589483e-01 12 62374390 62374535 146 + 0.417 0.253 -1.028
ENSG00000135655 E033 0.7480121 0.1075815680 7.719238e-01 8.528124e-01 12 62381135 62381200 66 + 0.187 0.252 0.546
ENSG00000135655 E034 75.9286467 0.0003780653 6.003526e-06 4.199032e-05 12 62381490 62381663 174 + 1.799 1.543 -0.868
ENSG00000135655 E035 69.0211060 0.0050006684 3.209700e-03 1.088382e-02 12 62383840 62383998 159 + 1.750 1.563 -0.634
ENSG00000135655 E036 0.6277178 0.0291626275 5.965420e-01 7.184695e-01 12 62383999 62384077 79 + 0.147 0.253 0.973
ENSG00000135655 E037 81.8780100 0.0003213374 1.838078e-02 4.796792e-02 12 62384078 62384302 225 + 1.811 1.705 -0.357
ENSG00000135655 E038 52.7564974 0.0005135948 4.755769e-02 1.048320e-01 12 62389431 62389514 84 + 1.625 1.513 -0.383
ENSG00000135655 E039 56.8515579 0.0086767463 6.772827e-02 1.395848e-01 12 62389605 62389699 95 + 1.658 1.535 -0.419
ENSG00000135655 E040 94.6550190 0.0005255394 2.977101e-04 1.370369e-03 12 62389797 62389988 192 + 1.881 1.712 -0.571
ENSG00000135655 E041 90.1940926 0.0003551145 7.194677e-03 2.173166e-02 12 62390864 62390979 116 + 1.855 1.738 -0.397
ENSG00000135655 E042 128.9550914 0.0003916667 7.307463e-02 1.484507e-01 12 62391157 62391429 273 + 2.000 1.948 -0.176
ENSG00000135655 E043 52.8037454 0.0004642623 4.782914e-01 6.167194e-01 12 62391816 62391886 71 + 1.613 1.590 -0.077
ENSG00000135655 E044 58.4326946 0.0060119232 6.538906e-01 7.642332e-01 12 62392272 62392361 90 + 1.644 1.698 0.182
ENSG00000135655 E045 43.3355511 0.0008685226 1.477345e-01 2.586080e-01 12 62392362 62392387 26 + 1.503 1.624 0.412
ENSG00000135655 E046 44.3885552 0.0005219682 1.068341e-01 2.008735e-01 12 62393053 62393074 22 + 1.510 1.640 0.442
ENSG00000135655 E047 85.2443344 0.0043694666 6.530539e-02 1.355484e-01 12 62393075 62393202 128 + 1.789 1.915 0.426
ENSG00000135655 E048 0.2944980 0.3780591016 1.000000e+00   12 62396284 62396294 11 + 0.103 0.000 -10.032
ENSG00000135655 E049 81.4506136 0.0058002720 4.703534e-02 1.038858e-01 12 62396295 62396398 104 + 1.766 1.907 0.477
ENSG00000135655 E050 0.1451727 0.0429527420 1.000000e+00   12 62401158 62401186 29 + 0.055 0.000 -9.033
ENSG00000135655 E051 68.6889783 0.0003842051 2.217530e-04 1.056946e-03 12 62401187 62401275 89 + 1.677 1.890 0.718
ENSG00000135655 E052 259.1193163 0.0240063226 8.344826e-02 1.649557e-01 12 62404193 62416389 12197 + 2.261 2.422 0.537
ENSG00000135655 E053 0.0000000       12 62417098 62417431 334 +