ENSG00000135632

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000389501 ENSG00000135632 HEK293_OSMI2_2hA HEK293_TMG_2hB SMYD5 protein_coding protein_coding 43.50134 72.17526 30.32816 2.478631 1.282149 -1.250571 38.211322 67.8122362 24.344888 2.6047052 0.1062875 -1.477547 0.85322500 0.9393333 0.80580000 -0.13353333 0.0007400722 0.0002812757 FALSE TRUE
ENST00000477038 ENSG00000135632 HEK293_OSMI2_2hA HEK293_TMG_2hB SMYD5 protein_coding retained_intron 43.50134 72.17526 30.32816 2.478631 1.282149 -1.250571 2.170742 0.9285167 3.105794 0.1221066 1.0395996 1.731145 0.06457917 0.0130000 0.09993333 0.08693333 0.0002812757 0.0002812757 FALSE FALSE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000135632 E001 0.000000       2 73214222 73214237 16 +      
ENSG00000135632 E002 0.000000       2 73214238 73214244 7 +      
ENSG00000135632 E003 32.798839 0.0098897354 6.248802e-02 1.308273e-01 2 73214245 73214266 22 + 1.537 1.423 -0.390
ENSG00000135632 E004 131.711398 0.0059054081 3.763676e-02 8.665021e-02 2 73214267 73214362 96 + 2.099 2.033 -0.220
ENSG00000135632 E005 1.817489 0.0096876199 3.484893e-03 1.168128e-02 2 73214567 73214589 23 + 0.719 0.255 -2.410
ENSG00000135632 E006 5.856839 0.0035317552 1.662068e-10 2.825501e-09 2 73214590 73214705 116 + 1.214 0.447 -3.096
ENSG00000135632 E007 5.470160 0.0030082320 2.648952e-08 3.014936e-07 2 73214706 73214749 44 + 1.154 0.477 -2.729
ENSG00000135632 E008 230.979417 0.0022375820 3.132132e-03 1.065397e-02 2 73218861 73218969 109 + 2.340 2.278 -0.209
ENSG00000135632 E009 4.112309 0.0059668158 6.253925e-03 1.927957e-02 2 73219917 73219951 35 + 0.897 0.531 -1.523
ENSG00000135632 E010 13.776612 0.0049708482 9.529949e-02 1.833305e-01 2 73219952 73220050 99 + 1.200 1.064 -0.486
ENSG00000135632 E011 170.405716 0.0020377258 1.393825e-01 2.471994e-01 2 73220051 73220069 19 + 2.176 2.158 -0.059
ENSG00000135632 E012 340.477794 0.0002091219 3.133926e-03 1.065854e-02 2 73220070 73220190 121 + 2.485 2.456 -0.096
ENSG00000135632 E013 363.356483 0.0001669256 1.934189e-03 7.022216e-03 2 73220661 73220782 122 + 2.511 2.481 -0.098
ENSG00000135632 E014 186.216633 0.0002385876 1.915070e-02 4.966179e-02 2 73221165 73221175 11 + 2.224 2.190 -0.112
ENSG00000135632 E015 283.943775 0.0001604912 8.876713e-05 4.692543e-04 2 73221176 73221234 59 + 2.425 2.368 -0.189
ENSG00000135632 E016 414.931675 0.0001619230 2.271830e-03 8.065049e-03 2 73221826 73221930 105 + 2.564 2.540 -0.081
ENSG00000135632 E017 322.210833 0.0012175149 1.125787e-01 2.093208e-01 2 73222755 73222817 63 + 2.441 2.435 -0.020
ENSG00000135632 E018 365.467499 0.0016364128 1.212921e-01 2.217673e-01 2 73223036 73223106 71 + 2.496 2.491 -0.018
ENSG00000135632 E019 5.106635 0.0032751111 3.225620e-06 2.396596e-05 2 73223107 73223167 61 + 1.084 0.505 -2.340
ENSG00000135632 E020 423.669791 0.0011532113 9.575818e-01 9.774065e-01 2 73223426 73223532 107 + 2.526 2.564 0.130
ENSG00000135632 E021 278.404470 0.0002055922 6.476309e-01 7.592733e-01 2 73223947 73224003 57 + 2.338 2.386 0.159
ENSG00000135632 E022 355.409361 0.0001429032 5.994205e-01 7.208136e-01 2 73224866 73224960 95 + 2.444 2.491 0.160
ENSG00000135632 E023 4.445060 0.0208365270 1.853077e-01 3.078829e-01 2 73225222 73225630 409 + 0.799 0.624 -0.723
ENSG00000135632 E024 302.938186 0.0011351187 1.563039e-01 2.702033e-01 2 73225631 73225701 71 + 2.351 2.429 0.259
ENSG00000135632 E025 2037.541182 0.0037556328 2.118424e-06 1.638747e-05 2 73225796 73227221 1426 + 3.123 3.270 0.490