ENSG00000135622

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000339773 ENSG00000135622 HEK293_OSMI2_2hA HEK293_TMG_2hB SEMA4F protein_coding protein_coding 8.822933 11.4557 5.874982 0.5648064 0.3726149 -0.9622142 1.3898409 3.170040 0.4213076 0.4009929 0.02544138 -2.8822560 0.1412250 0.2767667 0.07263333 -0.20413333 6.763644e-07 6.763644e-07 FALSE TRUE
ENST00000357877 ENSG00000135622 HEK293_OSMI2_2hA HEK293_TMG_2hB SEMA4F protein_coding protein_coding 8.822933 11.4557 5.874982 0.5648064 0.3726149 -0.9622142 3.5130129 3.203452 3.1531811 0.4635513 0.28908749 -0.0227476 0.4142417 0.2768667 0.53916667 0.26230000 1.700598e-03 6.763644e-07 FALSE TRUE
ENST00000446927 ENSG00000135622 HEK293_OSMI2_2hA HEK293_TMG_2hB SEMA4F protein_coding nonsense_mediated_decay 8.822933 11.4557 5.874982 0.5648064 0.3726149 -0.9622142 1.3076672 2.527433 0.3700904 0.5112609 0.14519715 -2.7389552 0.1421167 0.2260667 0.06130000 -0.16476667 2.853754e-02 6.763644e-07 FALSE TRUE
ENST00000453930 ENSG00000135622 HEK293_OSMI2_2hA HEK293_TMG_2hB SEMA4F protein_coding protein_coding 8.822933 11.4557 5.874982 0.5648064 0.3726149 -0.9622142 0.4896229 0.000000 0.5903555 0.0000000 0.31545152 5.9077452 0.0655875 0.0000000 0.09463333 0.09463333 1.052916e-01 6.763644e-07 FALSE FALSE
ENST00000611975 ENSG00000135622 HEK293_OSMI2_2hA HEK293_TMG_2hB SEMA4F protein_coding protein_coding 8.822933 11.4557 5.874982 0.5648064 0.3726149 -0.9622142 1.5147827 1.753348 0.6976486 0.4069221 0.23333849 -1.3172122 0.1656292 0.1501667 0.11930000 -0.03086667 7.966366e-01 6.763644e-07 FALSE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000135622 E001 0.1451727 0.0429216072 2.228786e-01   2 74654228 74654246 19 + 0.160 0.000 -10.222
ENSG00000135622 E002 0.1451727 0.0429216072 2.228786e-01   2 74654247 74654251 5 + 0.160 0.000 -12.101
ENSG00000135622 E003 1.3630300 0.0111384047 7.450929e-02 1.507541e-01 2 74654252 74654276 25 + 0.508 0.234 -1.636
ENSG00000135622 E004 1.6899071 0.0088284322 6.610845e-02 1.368971e-01 2 74654277 74654279 3 + 0.564 0.291 -1.484
ENSG00000135622 E005 3.5927647 0.1787507364 2.409541e-01 3.752931e-01 2 74654280 74654292 13 + 0.736 0.569 -0.714
ENSG00000135622 E006 7.0070342 0.2593608241 1.499696e-01 2.616310e-01 2 74654293 74654310 18 + 1.014 0.782 -0.881
ENSG00000135622 E007 14.5750724 0.1273768471 1.878105e-01 3.109772e-01 2 74654311 74654361 51 + 1.241 1.109 -0.471
ENSG00000135622 E008 40.2364725 0.0246568977 1.818314e-01 3.035293e-01 2 74654362 74654521 160 + 1.608 1.562 -0.155
ENSG00000135622 E009 0.9117205 0.0399528152 1.122943e-02 3.171533e-02 2 74655267 74655387 121 + 0.508 0.093 -3.222
ENSG00000135622 E010 62.0512883 0.0043592226 1.627242e-04 8.037235e-04 2 74656534 74656685 152 + 1.850 1.716 -0.454
ENSG00000135622 E011 58.3120371 0.0015804282 4.609592e-03 1.484598e-02 2 74657565 74657624 60 + 1.786 1.712 -0.249
ENSG00000135622 E012 26.5796674 0.0012827340 2.177773e-06 1.679924e-05 2 74657853 74657951 99 + 1.563 1.307 -0.883
ENSG00000135622 E013 32.7343434 0.0020634366 4.249697e-04 1.869985e-03 2 74662732 74662825 94 + 1.589 1.428 -0.552
ENSG00000135622 E014 51.9805201 0.0005671451 2.740961e-05 1.645645e-04 2 74673457 74673576 120 + 1.779 1.638 -0.480
ENSG00000135622 E015 69.3513418 0.0005588301 9.853974e-06 6.560785e-05 2 74673677 74673828 152 + 1.892 1.767 -0.422
ENSG00000135622 E016 92.4110997 0.0003065752 7.613225e-02 1.533207e-01 2 74674498 74674676 179 + 1.930 1.930 0.001
ENSG00000135622 E017 52.1476585 0.0005761102 1.656652e-01 2.825675e-01 2 74674888 74674922 35 + 1.687 1.683 -0.012
ENSG00000135622 E018 95.8987450 0.0004404525 2.520987e-03 8.826122e-03 2 74674923 74675035 113 + 1.979 1.934 -0.153
ENSG00000135622 E019 126.4101396 0.0007517746 2.084297e-05 1.287460e-04 2 74675162 74675384 223 + 2.118 2.044 -0.250
ENSG00000135622 E020 91.7120953 0.0003319875 1.260852e-02 3.498721e-02 2 74675525 74675634 110 + 1.945 1.918 -0.093
ENSG00000135622 E021 95.3843915 0.0003952873 9.300672e-02 1.798200e-01 2 74675749 74675909 161 + 1.941 1.946 0.015
ENSG00000135622 E022 61.9047996 0.0031720136 9.201976e-01 9.535865e-01 2 74679276 74679334 59 + 1.717 1.782 0.220
ENSG00000135622 E023 935.9001783 0.0032358185 2.756356e-19 1.622191e-17 2 74679599 74683853 4255 + 2.755 2.997 0.805