ENSG00000135535

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000310786 ENSG00000135535 HEK293_OSMI2_2hA HEK293_TMG_2hB CD164 protein_coding protein_coding 71.60797 22.30341 123.2597 5.046255 3.010522 2.465835 59.823964 10.641579 107.7251978 0.6456994 3.4603238 3.338350 0.75415000 0.5120333 0.873633333 0.36160000 1.430077e-05 1.430077e-05 FALSE TRUE
ENST00000324953 ENSG00000135535 HEK293_OSMI2_2hA HEK293_TMG_2hB CD164 protein_coding protein_coding 71.60797 22.30341 123.2597 5.046255 3.010522 2.465835 6.731074 2.416364 10.2926733 0.5516509 0.6512327 2.086151 0.08614167 0.1127000 0.083333333 -0.02936667 6.635021e-01 1.430077e-05 FALSE TRUE
ENST00000413644 ENSG00000135535 HEK293_OSMI2_2hA HEK293_TMG_2hB CD164 protein_coding protein_coding 71.60797 22.30341 123.2597 5.046255 3.010522 2.465835 2.326567 9.048617 0.2262098 4.2249461 0.2262098 -5.261150 0.12336250 0.3652667 0.001933333 -0.36333333 1.497091e-04 1.430077e-05 FALSE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000135535 E001 1.735802 0.0079559537 2.824771e-04 1.308389e-03 6 109366514 109366517 4 - 0.166 0.866 3.767
ENSG00000135535 E002 1458.098347 0.0093617970 1.035615e-17 5.077490e-16 6 109366518 109367278 761 - 2.898 3.367 1.559
ENSG00000135535 E003 1192.862214 0.0022974732 5.687528e-25 6.217456e-23 6 109367279 109367820 542 - 2.842 3.209 1.219
ENSG00000135535 E004 1056.944785 0.0007892117 2.500748e-01 3.860018e-01 6 109367821 109368325 505 - 2.844 2.951 0.353
ENSG00000135535 E005 705.461609 0.0028609335 9.713843e-11 1.717300e-09 6 109368326 109368683 358 - 2.695 2.604 -0.303
ENSG00000135535 E006 653.132243 0.0057808661 1.186185e-05 7.748403e-05 6 109368684 109368921 238 - 2.659 2.589 -0.234
ENSG00000135535 E007 507.728994 0.0127996089 5.981392e-04 2.526773e-03 6 109368922 109369017 96 - 2.553 2.454 -0.329
ENSG00000135535 E008 459.860925 0.0040259523 1.460295e-14 4.700110e-13 6 109370411 109370467 57 - 2.521 2.322 -0.664
ENSG00000135535 E009 29.417208 0.0013631273 4.759805e-06 3.405617e-05 6 109370468 109372869 2402 - 1.360 0.963 -1.423
ENSG00000135535 E010 4.148657 0.0849039940 5.600511e-01 6.880993e-01 6 109375887 109376073 187 - 0.551 0.745 0.834
ENSG00000135535 E011 463.962189 0.0015637508 4.188966e-22 3.375529e-20 6 109376074 109376112 39 - 2.524 2.332 -0.641
ENSG00000135535 E012 526.480239 0.0032194681 1.928147e-18 1.025784e-16 6 109377900 109377971 72 - 2.580 2.371 -0.697
ENSG00000135535 E013 516.393929 0.0001598339 6.986875e-37 1.972642e-34 6 109379579 109379662 84 - 2.570 2.382 -0.628
ENSG00000135535 E014 16.153461 0.0024177845 2.740725e-03 9.494261e-03 6 109379663 109380516 854 - 1.110 0.810 -1.126
ENSG00000135535 E015 3.719964 0.0139201447 1.048368e-02 2.993913e-02 6 109381504 109381709 206 - 0.593 0.000 -11.460
ENSG00000135535 E016 2.512107 0.0058229473 4.046705e-02 9.187686e-02 6 109381710 109381901 192 - 0.474 0.000 -10.903
ENSG00000135535 E017 614.866621 0.0030613594 2.376661e-14 7.393290e-13 6 109382204 109382572 369 - 2.641 2.497 -0.481
ENSG00000135535 E018 1.511326 0.0245100348 6.823563e-01 7.858094e-01 6 109382692 109383505 814 - 0.312 0.281 -0.207