ENSG00000135476

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000257934 ENSG00000135476 HEK293_OSMI2_2hA HEK293_TMG_2hB ESPL1 protein_coding protein_coding 33.4473 41.40889 26.5121 3.481295 1.318516 -0.643094 17.070155 15.786305 15.5775165 3.9521059 0.7523345 -0.01919601 0.5235292 0.3736667 0.5887000 0.21503333 1.315223e-01 2.840177e-21 FALSE TRUE
ENST00000549154 ENSG00000135476 HEK293_OSMI2_2hA HEK293_TMG_2hB ESPL1 protein_coding retained_intron 33.4473 41.40889 26.5121 3.481295 1.318516 -0.643094 3.447937 3.157921 3.1312347 0.3172678 0.1109210 -0.01220443 0.1056000 0.0768000 0.1188000 0.04200000 5.878861e-02 2.840177e-21 FALSE TRUE
ENST00000552600 ENSG00000135476 HEK293_OSMI2_2hA HEK293_TMG_2hB ESPL1 protein_coding retained_intron 33.4473 41.40889 26.5121 3.481295 1.318516 -0.643094 1.654755 4.618120 0.8813453 1.1193522 0.8813453 -2.37636990 0.0438875 0.1115667 0.0320000 -0.07956667 1.389239e-01 2.840177e-21 FALSE FALSE
MSTRG.7351.1 ENSG00000135476 HEK293_OSMI2_2hA HEK293_TMG_2hB ESPL1 protein_coding   33.4473 41.40889 26.5121 3.481295 1.318516 -0.643094 3.348198 3.407416 2.7694421 0.5644556 0.1944046 -0.29811041 0.1035458 0.0823000 0.1057667 0.02346667 5.782393e-01 2.840177e-21 TRUE TRUE
MSTRG.7351.4 ENSG00000135476 HEK293_OSMI2_2hA HEK293_TMG_2hB ESPL1 protein_coding   33.4473 41.40889 26.5121 3.481295 1.318516 -0.643094 4.262458 11.345589 0.0000000 1.8568272 0.0000000 -10.14918677 0.1078917 0.2789333 0.0000000 -0.27893333 2.840177e-21 2.840177e-21 FALSE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000135476 E001 0.5106578 0.2191495874 9.643777e-01 9.815638e-01 12 53268261 53268262 2 + 0.156 0.174 0.198
ENSG00000135476 E002 10.9895373 0.0208223617 1.203025e-01 2.204110e-01 12 53268263 53268298 36 + 1.169 0.983 -0.677
ENSG00000135476 E003 55.3715723 0.0264918352 2.761259e-04 1.282515e-03 12 53268299 53268332 34 + 1.909 1.587 -1.089
ENSG00000135476 E004 61.0656201 0.0195771822 4.773890e-04 2.073454e-03 12 53268333 53268334 2 + 1.927 1.651 -0.932
ENSG00000135476 E005 93.9242539 0.0037398280 1.900565e-05 1.184864e-04 12 53268335 53268377 43 + 2.071 1.867 -0.684
ENSG00000135476 E006 18.2340818 0.0010390669 6.349724e-01 7.493540e-01 12 53268378 53268406 29 + 1.281 1.236 -0.156
ENSG00000135476 E007 141.9387374 0.0090510446 5.437357e-05 3.035840e-04 12 53268755 53268847 93 + 2.258 2.039 -0.730
ENSG00000135476 E008 14.9842233 0.0213211057 4.034044e-02 9.165156e-02 12 53268848 53268928 81 + 1.319 1.090 -0.812
ENSG00000135476 E009 296.7501212 0.0140251918 2.517230e-04 1.182430e-03 12 53269024 53269525 502 + 2.575 2.363 -0.709
ENSG00000135476 E010 169.6033614 0.0102132859 1.142821e-03 4.446946e-03 12 53269526 53269701 176 + 2.313 2.135 -0.593
ENSG00000135476 E011 315.3934720 0.0042442975 4.810184e-08 5.208161e-07 12 53269702 53270085 384 + 2.595 2.393 -0.670
ENSG00000135476 E012 174.2861414 0.0026811066 1.377215e-12 3.297640e-11 12 53270378 53270482 105 + 2.369 2.109 -0.870
ENSG00000135476 E013 194.3440906 0.0058281193 2.925896e-05 1.745365e-04 12 53270678 53270798 121 + 2.375 2.190 -0.619
ENSG00000135476 E014 213.6498245 0.0036888854 1.236256e-04 6.304647e-04 12 53272721 53272857 137 + 2.393 2.247 -0.489
ENSG00000135476 E015 14.3006690 0.0012366574 1.621090e-04 8.010607e-04 12 53274081 53274466 386 + 1.353 1.015 -1.204
ENSG00000135476 E016 228.4636010 0.0076120107 2.423915e-02 6.030407e-02 12 53274817 53275010 194 + 2.398 2.292 -0.355
ENSG00000135476 E017 263.3403950 0.0051289978 1.705609e-03 6.300280e-03 12 53276620 53276859 240 + 2.472 2.345 -0.424
ENSG00000135476 E018 199.4977386 0.0089342562 4.080886e-02 9.252838e-02 12 53277083 53277227 145 + 2.338 2.235 -0.345
ENSG00000135476 E019 204.0371682 0.0030478659 1.201105e-02 3.355736e-02 12 53277470 53277608 139 + 2.342 2.248 -0.314
ENSG00000135476 E020 176.8097251 0.0053489116 9.396128e-01 9.660863e-01 12 53277821 53277960 140 + 2.222 2.217 -0.016
ENSG00000135476 E021 0.8168978 0.0141750589 4.977167e-02 1.087567e-01 12 53279705 53279731 27 + 0.435 0.096 -2.807
ENSG00000135476 E022 198.6380351 0.0009380182 3.491450e-05 2.041811e-04 12 53279732 53279866 135 + 2.348 2.222 -0.420
ENSG00000135476 E023 220.0351990 0.0001695829 5.192073e-06 3.681222e-05 12 53281507 53281626 120 + 2.386 2.268 -0.395
ENSG00000135476 E024 0.7425998 0.1017571102 3.238461e-01 4.671788e-01 12 53282250 53282263 14 + 0.360 0.172 -1.410
ENSG00000135476 E025 250.8036164 0.0004120124 1.460937e-04 7.303411e-04 12 53282264 53282435 172 + 2.430 2.331 -0.327
ENSG00000135476 E026 228.8859784 0.0023090205 1.828037e-01 3.047216e-01 12 53283129 53283257 129 + 2.360 2.313 -0.156
ENSG00000135476 E027 229.3176910 0.0003852030 8.665208e-01 9.180630e-01 12 53283382 53283538 157 + 2.334 2.325 -0.030
ENSG00000135476 E028 176.8634021 0.0002390471 6.926910e-01 7.938628e-01 12 53284058 53284167 110 + 2.228 2.211 -0.055
ENSG00000135476 E029 762.5017824 0.0003616945 2.332968e-02 5.843742e-02 12 53285924 53286912 989 + 2.825 2.858 0.108
ENSG00000135476 E030 20.1810608 0.0220650992 1.538145e-04 7.640792e-04 12 53287256 53287880 625 + 1.511 1.122 -1.361
ENSG00000135476 E031 10.1503554 0.0016417243 4.197534e-03 1.369730e-02 12 53287881 53287971 91 + 1.194 0.897 -1.088
ENSG00000135476 E032 522.2162429 0.0015816777 8.088674e-01 8.786259e-01 12 53287972 53288341 370 + 2.682 2.687 0.017
ENSG00000135476 E033 347.1471113 0.0002155464 3.942926e-03 1.298230e-02 12 53288538 53288699 162 + 2.466 2.522 0.187
ENSG00000135476 E034 11.5885276 0.0015308252 3.946953e-04 1.754225e-03 12 53288700 53289089 390 + 1.271 0.923 -1.259
ENSG00000135476 E035 443.5998353 0.0003655878 5.000010e-06 3.558900e-05 12 53289090 53289303 214 + 2.552 2.639 0.289
ENSG00000135476 E036 410.9585889 0.0007411992 2.095032e-06 1.622659e-05 12 53289404 53289594 191 + 2.507 2.612 0.349
ENSG00000135476 E037 98.7097176 0.0009708971 6.524803e-13 1.652104e-11 12 53289595 53290084 490 + 2.136 1.857 -0.936
ENSG00000135476 E038 334.3822995 0.0001706635 2.873686e-08 3.247114e-07 12 53290085 53290212 128 + 2.409 2.524 0.383
ENSG00000135476 E039 41.4184661 0.0005353413 1.169039e-07 1.172735e-06 12 53290213 53290346 134 + 1.772 1.477 -1.002
ENSG00000135476 E040 344.2260907 0.0016386370 1.831540e-05 1.146245e-04 12 53290347 53290469 123 + 2.421 2.540 0.397
ENSG00000135476 E041 359.0943118 0.0010290533 8.917559e-14 2.553514e-12 12 53290841 53290996 156 + 2.387 2.576 0.629
ENSG00000135476 E042 415.2445394 0.0038779702 8.732628e-07 7.344921e-06 12 53291690 53291860 171 + 2.461 2.637 0.585
ENSG00000135476 E043 365.6559228 0.0068674933 1.246707e-03 4.799043e-03 12 53291984 53292088 105 + 2.427 2.575 0.496
ENSG00000135476 E044 397.5744933 0.0045140823 1.028011e-03 4.055424e-03 12 53292278 53292393 116 + 2.479 2.605 0.421
ENSG00000135476 E045 284.7743176 0.0022834639 1.857302e-09 2.618009e-08 12 53292574 53292657 84 + 2.282 2.478 0.654
ENSG00000135476 E046 319.6704873 0.0062724716 4.619586e-07 4.114366e-06 12 53292806 53292970 165 + 2.311 2.535 0.749
ENSG00000135476 E047 427.2625261 0.0046271578 1.197995e-10 2.087725e-09 12 53293273 53293638 366 + 2.419 2.666 0.822