ENSG00000135414

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000257868 ENSG00000135414 HEK293_OSMI2_2hA HEK293_TMG_2hB GDF11 protein_coding protein_coding 13.0449 19.51619 9.144831 0.6413743 0.1660602 -1.092805 3.352615 4.106739 2.994885 0.1098263 0.05234288 -0.4541923 0.2744625 0.2107667 0.3279000 0.1171333 0.0005605259 0.0005605259 FALSE TRUE
ENST00000546799 ENSG00000135414 HEK293_OSMI2_2hA HEK293_TMG_2hB GDF11 protein_coding protein_coding 13.0449 19.51619 9.144831 0.6413743 0.1660602 -1.092805 3.269218 6.675266 2.146660 1.1532399 0.12127782 -1.6321863 0.2295542 0.3389667 0.2349667 -0.1040000 0.3251295535 0.0005605259 FALSE TRUE
MSTRG.7425.5 ENSG00000135414 HEK293_OSMI2_2hA HEK293_TMG_2hB GDF11 protein_coding   13.0449 19.51619 9.144831 0.6413743 0.1660602 -1.092805 6.248286 8.632958 3.998364 0.6611345 0.26770702 -1.1085115 0.4837083 0.4451667 0.4365667 -0.0086000 0.9771053114 0.0005605259 FALSE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000135414 E001 1.878988 0.0076467424 3.126374e-03 1.063715e-02 12 55743122 55743398 277 + 0.721 0.223 -2.663
ENSG00000135414 E002 3.472951 0.0046258459 6.790998e-02 1.399015e-01 12 55743399 55743434 36 + 0.793 0.511 -1.215
ENSG00000135414 E003 14.545348 0.0114636954 6.777815e-01 7.824054e-01 12 55743435 55743476 42 + 1.182 1.126 -0.199
ENSG00000135414 E004 26.160113 0.0009127768 5.960360e-01 7.180268e-01 12 55743477 55743502 26 + 1.366 1.394 0.095
ENSG00000135414 E005 162.680943 0.0002675250 9.185010e-02 1.780089e-01 12 55743503 55743761 259 + 2.137 2.176 0.130
ENSG00000135414 E006 115.556487 0.0002977166 3.352118e-01 4.789856e-01 12 55748586 55748647 62 + 2.001 2.024 0.075
ENSG00000135414 E007 184.347671 0.0002102828 6.898584e-03 2.096130e-02 12 55748648 55748848 201 + 2.175 2.239 0.214
ENSG00000135414 E008 174.070451 0.0002903580 1.546897e-05 9.842315e-05 12 55748849 55748983 135 + 2.111 2.228 0.390
ENSG00000135414 E009 282.149434 0.0003316350 1.783683e-07 1.726426e-06 12 55749502 55749883 382 + 2.322 2.432 0.366
ENSG00000135414 E010 384.151090 0.0002308459 9.555979e-04 3.807179e-03 12 55749884 55751228 1345 + 2.588 2.516 -0.238
ENSG00000135414 E011 286.068067 0.0013004989 8.428331e-01 9.020824e-01 12 55751229 55752128 900 + 2.425 2.409 -0.053
ENSG00000135414 E012 291.747059 0.0025287233 1.193788e-08 1.450950e-07 12 55752129 55757012 4884 + 2.538 2.365 -0.578
ENSG00000135414 E013 9.176162 0.0259616170 6.761182e-01 7.811820e-01 12 55757013 55757264 252 + 1.019 0.962 -0.212