ENSG00000135336

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000257789 ENSG00000135336 HEK293_OSMI2_2hA HEK293_TMG_2hB ORC3 protein_coding protein_coding 25.63333 14.97447 34.98147 1.118728 0.5275364 1.223535 7.129455 2.5915177 10.940733 0.20189831 0.4821882 2.0736020 0.24212500 0.1764000 0.31280000 0.13640000 5.497544e-03 2.729131e-12 FALSE TRUE
ENST00000392844 ENSG00000135336 HEK293_OSMI2_2hA HEK293_TMG_2hB ORC3 protein_coding protein_coding 25.63333 14.97447 34.98147 1.118728 0.5275364 1.223535 6.642412 2.3818037 11.050992 0.25299353 1.1797433 2.2093098 0.23248333 0.1595667 0.31523333 0.15566667 2.132733e-03 2.729131e-12 FALSE TRUE
ENST00000468486 ENSG00000135336 HEK293_OSMI2_2hA HEK293_TMG_2hB ORC3 protein_coding retained_intron 25.63333 14.97447 34.98147 1.118728 0.5275364 1.223535 3.491144 4.0310021 2.491360 0.49987669 0.4495319 -0.6920004 0.18821250 0.2683667 0.07143333 -0.19693333 1.836798e-07 2.729131e-12   FALSE
ENST00000681069 ENSG00000135336 HEK293_OSMI2_2hA HEK293_TMG_2hB ORC3 protein_coding retained_intron 25.63333 14.97447 34.98147 1.118728 0.5275364 1.223535 2.026990 0.2976083 2.748801 0.06589382 0.1420070 3.1648752 0.06504583 0.0208000 0.07853333 0.05773333 4.165599e-06 2.729131e-12 FALSE TRUE
MSTRG.28634.2 ENSG00000135336 HEK293_OSMI2_2hA HEK293_TMG_2hB ORC3 protein_coding   25.63333 14.97447 34.98147 1.118728 0.5275364 1.223535 2.859012 4.9064545 2.100910 0.93044563 0.1075683 -1.2197531 0.15177083 0.3229667 0.06000000 -0.26296667 2.729131e-12 2.729131e-12   FALSE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000135336 E001 0.1515154 0.0424795679 1.000000e+00   6 87590067 87590103 37 + 0.066 0.000 -8.978
ENSG00000135336 E002 0.2966881 0.0290785164 1.000000e+00   6 87590104 87590106 3 + 0.124 0.000 -9.967
ENSG00000135336 E003 0.2966881 0.0290785164 1.000000e+00   6 87590107 87590111 5 + 0.124 0.000 -9.967
ENSG00000135336 E004 2.0787794 0.0071905079 1.998413e-02 5.145199e-02 6 87590112 87590129 18 + 0.520 0.000 -12.775
ENSG00000135336 E005 7.7808822 0.0108669616 3.537472e-01 4.980073e-01 6 87590130 87590134 5 + 0.908 0.788 -0.466
ENSG00000135336 E006 7.7808822 0.0108669616 3.537472e-01 4.980073e-01 6 87590135 87590135 1 + 0.908 0.788 -0.466
ENSG00000135336 E007 121.6201052 0.0057874323 2.897187e-01 4.304373e-01 6 87590136 87590192 57 + 2.002 2.071 0.231
ENSG00000135336 E008 152.1226801 0.0015816512 4.662237e-02 1.031577e-01 6 87594353 87594407 55 + 2.093 2.183 0.300
ENSG00000135336 E009 13.7334277 0.0207816336 1.650161e-08 1.953863e-07 6 87594527 87594541 15 + 0.851 1.492 2.299
ENSG00000135336 E010 45.3531893 0.0157917839 7.734246e-17 3.372997e-15 6 87594542 87594613 72 + 1.325 2.003 2.309
ENSG00000135336 E011 45.1554634 0.0083278045 3.020871e-43 1.271767e-40 6 87595172 87595437 266 + 1.131 2.099 3.314
ENSG00000135336 E012 151.6213232 0.0002236809 1.492350e-15 5.517590e-14 6 87601784 87601881 98 + 2.168 1.878 -0.974
ENSG00000135336 E013 164.5018033 0.0002631653 2.195529e-15 7.933175e-14 6 87603384 87603528 145 + 2.200 1.924 -0.926
ENSG00000135336 E014 110.4104305 0.0002905185 1.259939e-11 2.581009e-10 6 87605917 87606021 105 + 2.031 1.740 -0.978
ENSG00000135336 E015 119.1324597 0.0004015801 1.305202e-11 2.667960e-10 6 87607673 87607824 152 + 2.063 1.783 -0.940
ENSG00000135336 E016 127.9150602 0.0002523842 9.179304e-06 6.154415e-05 6 87609096 87609229 134 + 2.074 1.917 -0.529
ENSG00000135336 E017 0.5944058 0.4209494535 1.652393e-01 2.819888e-01 6 87609230 87609531 302 + 0.066 0.371 3.029
ENSG00000135336 E018 141.2589767 0.0002531191 2.884700e-02 6.969745e-02 6 87612089 87612248 160 + 2.098 2.037 -0.206
ENSG00000135336 E019 106.1486937 0.0052162728 7.477601e-02 1.511646e-01 6 87616314 87616427 114 + 1.978 1.894 -0.283
ENSG00000135336 E020 110.5235089 0.0009993559 3.711362e-03 1.233022e-02 6 87621354 87621487 134 + 2.002 1.892 -0.369
ENSG00000135336 E021 75.0117227 0.0016050041 3.579376e-02 8.318363e-02 6 87621950 87622013 64 + 1.834 1.740 -0.319
ENSG00000135336 E022 106.2633179 0.0060771455 4.023475e-01 5.460763e-01 6 87634845 87634961 117 + 1.970 1.943 -0.092
ENSG00000135336 E023 89.9981627 0.0029051474 7.293176e-01 8.215237e-01 6 87636407 87636486 80 + 1.884 1.919 0.116
ENSG00000135336 E024 3.5408960 0.0043714609 4.913877e-01 6.286554e-01 6 87650930 87651392 463 + 0.561 0.686 0.549
ENSG00000135336 E025 125.6707745 0.0002327673 2.021828e-01 3.289302e-01 6 87653116 87653249 134 + 2.018 2.081 0.211
ENSG00000135336 E026 24.0480395 0.0007651704 7.139181e-01 8.102768e-01 6 87656903 87656905 3 + 1.335 1.320 -0.049
ENSG00000135336 E027 93.4876678 0.0025731036 4.581189e-01 5.984493e-01 6 87656906 87656982 77 + 1.894 1.946 0.175
ENSG00000135336 E028 17.1770771 0.0010350051 3.846507e-01 5.288089e-01 6 87656983 87657388 406 + 1.167 1.264 0.342
ENSG00000135336 E029 112.0179389 0.0010370981 3.728062e-01 5.172283e-01 6 87657921 87658018 98 + 1.971 2.024 0.178
ENSG00000135336 E030 124.9514916 0.0004305514 3.357387e-02 7.891694e-02 6 87663003 87663144 142 + 2.007 2.101 0.317
ENSG00000135336 E031 134.3560932 0.0002247062 1.216719e-02 3.393104e-02 6 87664743 87664859 117 + 2.036 2.139 0.346
ENSG00000135336 E032 93.3025678 0.0003501066 1.888482e-03 6.878551e-03 6 87665754 87665833 80 + 1.866 2.011 0.485
ENSG00000135336 E033 4.5897609 0.0039333998 2.158625e-01 3.454424e-01 6 87667011 87667017 7 + 0.633 0.831 0.810
ENSG00000135336 E034 156.8682669 0.0002572115 3.741628e-07 3.393203e-06 6 87667018 87667453 436 + 2.080 2.256 0.586