ENSG00000135333

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000369303 ENSG00000135333 HEK293_OSMI2_2hA HEK293_TMG_2hB EPHA7 protein_coding protein_coding 4.431034 0.9826916 5.565998 0.02675729 0.2798472 2.489813 1.841043 0.7864817 2.3666735 0.04875025 0.3919683 1.577231 0.50316250 0.8038667 0.4211667 -0.3827 3.406558e-03 2.95888e-07 FALSE TRUE
ENST00000680224 ENSG00000135333 HEK293_OSMI2_2hA HEK293_TMG_2hB EPHA7 protein_coding protein_coding 4.431034 0.9826916 5.565998 0.02675729 0.2798472 2.489813 0.503068 0.0205001 0.5563803 0.02050010 0.3053054 4.214885 0.07692917 0.0207000 0.1052000 0.0845 7.559002e-01 2.95888e-07 FALSE TRUE
MSTRG.28658.9 ENSG00000135333 HEK293_OSMI2_2hA HEK293_TMG_2hB EPHA7 protein_coding   4.431034 0.9826916 5.565998 0.02675729 0.2798472 2.489813 1.339534 0.0000000 1.5385726 0.00000000 0.1270370 7.274795 0.18396250 0.0000000 0.2790000 0.2790 2.958880e-07 2.95888e-07 FALSE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000135333 E001 0.6663248 0.0427374013 4.989883e-04 2.156243e-03 6 93240020 93240037 18 - 0.000 0.597 14.345
ENSG00000135333 E002 0.6663248 0.0427374013 4.989883e-04 2.156243e-03 6 93240038 93240042 5 - 0.000 0.597 16.794
ENSG00000135333 E003 101.2117872 0.0200799462 1.519274e-05 9.683631e-05 6 93240043 93241730 1688 - 1.816 2.162 1.162
ENSG00000135333 E004 64.6065710 0.0015513494 8.203827e-01 8.866952e-01 6 93241731 93242521 791 - 1.698 1.753 0.187
ENSG00000135333 E005 68.2037605 0.0005100306 3.958136e-02 9.025721e-02 6 93242522 93243540 1019 - 1.747 1.678 -0.233
ENSG00000135333 E006 28.9701680 0.0037620143 7.843428e-01 8.616799e-01 6 93245298 93245453 156 - 1.372 1.393 0.070
ENSG00000135333 E007 0.7792333 0.0183076648 8.934717e-01 9.358972e-01 6 93245454 93246076 623 - 0.196 0.241 0.374
ENSG00000135333 E008 30.1691265 0.0058113600 6.159577e-01 7.341044e-01 6 93246792 93246985 194 - 1.393 1.393 -0.001
ENSG00000135333 E009 1.0330470 0.0119821399 2.107889e-01 3.394883e-01 6 93246986 93247564 579 - 0.301 0.000 -14.709
ENSG00000135333 E010 0.9652040 0.2357272520 4.159024e-01 5.592489e-01 6 93253925 93254577 653 - 0.197 0.396 1.376
ENSG00000135333 E011 0.1451727 0.0428659828 1.000000e+00   6 93254578 93254646 69 - 0.058 0.000 -12.107
ENSG00000135333 E012 26.3158513 0.0213903740 2.087321e-01 3.369835e-01 6 93254647 93254796 150 - 1.353 1.256 -0.341
ENSG00000135333 E013 28.3703295 0.0007205634 1.208109e-01 2.211094e-01 6 93255828 93256037 210 - 1.380 1.291 -0.312
ENSG00000135333 E014 18.2022063 0.0010060017 2.029245e-01 3.298733e-01 6 93257462 93257523 62 - 1.200 1.109 -0.326
ENSG00000135333 E015 33.0860645 0.0006537209 1.285127e-01 2.320822e-01 6 93258099 93258284 186 - 1.443 1.366 -0.266
ENSG00000135333 E016 23.1312786 0.0009047603 4.126766e-01 5.562379e-01 6 93259354 93259467 114 - 1.289 1.256 -0.114
ENSG00000135333 E017 10.8210360 0.0015722555 8.778056e-01 9.255263e-01 6 93259468 93259479 12 - 0.968 1.027 0.217
ENSG00000135333 E018 1.0695787 0.0115922215 8.282910e-01 8.920872e-01 6 93259480 93259956 477 - 0.269 0.241 -0.210
ENSG00000135333 E019 0.1472490 0.0428137568 1.000000e+00   6 93259957 93259963 7 - 0.058 0.000 -12.108
ENSG00000135333 E020 0.1515154 0.0423017733 1.000000e+00   6 93259964 93259991 28 - 0.058 0.000 -12.109
ENSG00000135333 E021 0.1515154 0.0423017733 1.000000e+00   6 93259992 93259992 1 - 0.058 0.000 -12.109
ENSG00000135333 E022 2.0527536 0.2347857834 9.926061e-01 9.994698e-01 6 93259993 93260779 787 - 0.412 0.399 -0.069
ENSG00000135333 E023 0.0000000       6 93262877 93262955 79 -      
ENSG00000135333 E024 15.9623804 0.0244619856 4.516783e-01 5.926084e-01 6 93263860 93263915 56 - 1.105 1.236 0.466
ENSG00000135333 E025 0.0000000       6 93264289 93264593 305 -      
ENSG00000135333 E026 16.0444065 0.0019113840 3.571757e-01 5.015776e-01 6 93264594 93264696 103 - 1.105 1.238 0.476
ENSG00000135333 E027 6.6485948 0.0229771961 8.518960e-01 9.081861e-01 6 93264697 93264702 6 - 0.778 0.841 0.247
ENSG00000135333 E028 4.7100563 0.0127708510 4.360529e-01 5.781820e-01 6 93269477 93269491 15 - 0.632 0.792 0.659
ENSG00000135333 E029 25.6271032 0.0011273871 1.121123e-01 2.086380e-01 6 93269492 93269660 169 - 1.340 1.238 -0.356
ENSG00000135333 E030 22.0740964 0.0009253804 8.589248e-02 1.688132e-01 6 93272298 93272422 125 - 1.282 1.157 -0.444
ENSG00000135333 E031 0.0000000       6 93282219 93284384 2166 -      
ENSG00000135333 E032 0.7834997 0.0185059104 8.924218e-01 9.352530e-01 6 93343083 93343808 726 - 0.196 0.241 0.374
ENSG00000135333 E033 0.6664265 0.0176742197 1.413134e-01 2.498381e-01 6 93355142 93355353 212 - 0.109 0.395 2.375
ENSG00000135333 E034 2.4681096 0.0088737730 1.922206e-01 3.165446e-01 6 93355354 93356484 1131 - 0.410 0.672 1.236
ENSG00000135333 E035 0.2903454 0.3448957981 1.000000e+00   6 93356485 93356557 73 - 0.109 0.000 -12.600
ENSG00000135333 E036 0.6537541 0.4656945954 2.483478e-01 3.839774e-01 6 93356558 93356716 159 - 0.109 0.397 2.393
ENSG00000135333 E037 38.7581197 0.0010466065 1.318527e-01 2.366932e-01 6 93356717 93357052 336 - 1.509 1.442 -0.231
ENSG00000135333 E038 22.6906516 0.0043095876 5.299315e-01 6.626575e-01 6 93358256 93358411 156 - 1.276 1.257 -0.067
ENSG00000135333 E039 0.1515154 0.0423017733 1.000000e+00   6 93358412 93358872 461 - 0.058 0.000 -12.109
ENSG00000135333 E040 3.4273392 0.1314012272 9.044709e-01 9.431712e-01 6 93409654 93410500 847 - 0.571 0.590 0.083
ENSG00000135333 E041 37.2098941 0.0006868162 3.037152e-01 4.456781e-01 6 93410501 93410822 322 - 1.486 1.454 -0.110
ENSG00000135333 E042 37.6771325 0.0006861496 3.464075e-01 4.905331e-01 6 93410823 93411170 348 - 1.490 1.465 -0.085
ENSG00000135333 E043 16.1550383 0.0011462066 1.433206e-01 2.525289e-01 6 93414703 93414767 65 - 1.151 1.027 -0.448
ENSG00000135333 E044 0.0000000       6 93416955 93417160 206 -      
ENSG00000135333 E045 0.2944980 0.3646370257 1.000000e+00   6 93417261 93417382 122 - 0.109 0.000 -12.582
ENSG00000135333 E046 29.0838321 0.0046227141 1.261617e-01 2.288115e-01 6 93419245 93419687 443 - 1.388 1.292 -0.335