ENSG00000135250

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000357311 ENSG00000135250 HEK293_OSMI2_2hA HEK293_TMG_2hB SRPK2 protein_coding protein_coding 14.42464 6.830452 23.97923 0.4638958 0.4494882 1.810223 0.7697070 0.09004046 1.065252 0.09004046 0.2767446 3.426019 0.05535833 0.013400000 0.04446667 0.03106667 2.898213e-01 1.425787e-06 FALSE  
ENST00000393651 ENSG00000135250 HEK293_OSMI2_2hA HEK293_TMG_2hB SRPK2 protein_coding protein_coding 14.42464 6.830452 23.97923 0.4638958 0.4494882 1.810223 9.9566381 5.14251651 15.617384 0.13910849 0.4787900 1.600727 0.71290417 0.760400000 0.65100000 -0.10940000 3.031331e-01 1.425787e-06 FALSE  
ENST00000477925 ENSG00000135250 HEK293_OSMI2_2hA HEK293_TMG_2hB SRPK2 protein_coding protein_coding 14.42464 6.830452 23.97923 0.4638958 0.4494882 1.810223 1.2949319 0.43194385 2.673513 0.21936606 0.4416475 2.602188 0.08338333 0.067266667 0.11130000 0.04403333 6.695438e-01 1.425787e-06 FALSE  
ENST00000485455 ENSG00000135250 HEK293_OSMI2_2hA HEK293_TMG_2hB SRPK2 protein_coding processed_transcript 14.42464 6.830452 23.97923 0.4638958 0.4494882 1.810223 0.0754691 0.60375283 0.000000 0.60375283 0.0000000 -5.939586 0.00982500 0.078600000 0.00000000 -0.07860000 6.294031e-01 1.425787e-06    
MSTRG.30452.9 ENSG00000135250 HEK293_OSMI2_2hA HEK293_TMG_2hB SRPK2 protein_coding   14.42464 6.830452 23.97923 0.4638958 0.4494882 1.810223 0.5812816 0.02664309 1.419636 0.01599702 0.1231347 5.285963 0.02687500 0.003633333 0.05926667 0.05563333 1.425787e-06 1.425787e-06 TRUE  
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000135250 E001 8.0641714 0.0289384269 4.868064e-03 1.556287e-02 7 105110704 105111188 485 - 1.000 0.525 -1.939
ENSG00000135250 E002 2.7149163 0.0629263776 5.737183e-02 1.221145e-01 7 105115240 105115268 29 - 0.609 0.201 -2.380
ENSG00000135250 E003 5.3848939 0.0080652535 1.660586e-02 4.407246e-02 7 105115269 105115386 118 - 0.841 0.442 -1.752
ENSG00000135250 E004 5.8788273 0.0254584200 1.139395e-01 2.112622e-01 7 105115387 105115503 117 - 0.853 0.595 -1.060
ENSG00000135250 E005 6.3310421 0.0025067507 1.414714e-01 2.500357e-01 7 105116374 105116376 3 - 0.863 0.656 -0.835
ENSG00000135250 E006 283.9515960 0.0028014817 1.385131e-17 6.677074e-16 7 105116377 105117295 919 - 2.311 2.589 0.929
ENSG00000135250 E007 70.8226964 0.0003678198 6.661110e-03 2.034218e-02 7 105117296 105117348 53 - 1.765 1.902 0.462
ENSG00000135250 E008 46.8259144 0.0004583013 5.052385e-02 1.100919e-01 7 105117349 105117350 2 - 1.595 1.718 0.417
ENSG00000135250 E009 52.2828474 0.0004436483 2.010372e-02 5.170358e-02 7 105117351 105117366 16 - 1.636 1.773 0.462
ENSG00000135250 E010 55.6711852 0.0004053375 3.523668e-02 8.209834e-02 7 105117367 105117387 21 - 1.668 1.790 0.412
ENSG00000135250 E011 95.2578889 0.0022694787 2.051764e-06 1.592056e-05 7 105117388 105117511 124 - 1.858 2.078 0.738
ENSG00000135250 E012 53.8960834 0.0005064172 2.812106e-07 2.617655e-06 7 105117512 105117525 14 - 1.593 1.866 0.924
ENSG00000135250 E013 63.5306663 0.0004634564 1.132120e-06 9.292352e-06 7 105117526 105117579 54 - 1.676 1.918 0.817
ENSG00000135250 E014 97.5357464 0.0026644841 4.170353e-02 9.419504e-02 7 105117580 105117707 128 - 1.909 2.015 0.356
ENSG00000135250 E015 78.8678596 0.0003731944 9.546075e-01 9.755399e-01 7 105117708 105117755 48 - 1.847 1.859 0.040
ENSG00000135250 E016 174.5234068 0.0002132775 8.317945e-01 8.945852e-01 7 105117756 105118022 267 - 2.191 2.199 0.027
ENSG00000135250 E017 11.6151397 0.0014762399 5.551046e-01 6.839996e-01 7 105125782 105125874 93 - 1.030 1.106 0.276
ENSG00000135250 E018 85.9252314 0.0016600951 5.062653e-01 6.419150e-01 7 105126248 105126340 93 - 1.893 1.876 -0.058
ENSG00000135250 E019 80.1840977 0.0060334949 3.073167e-01 4.495319e-01 7 105126993 105127062 70 - 1.872 1.828 -0.151
ENSG00000135250 E020 77.1799986 0.0009745866 2.472851e-01 3.827434e-01 7 105132791 105132898 108 - 1.855 1.814 -0.138
ENSG00000135250 E021 83.0510903 0.0003363725 1.268939e-01 2.298203e-01 7 105133004 105133104 101 - 1.890 1.837 -0.180
ENSG00000135250 E022 249.7844130 0.0013871448 1.192925e-02 3.336327e-02 7 105142008 105142490 483 - 2.365 2.306 -0.199
ENSG00000135250 E023 112.6640610 0.0029013812 6.055809e-03 1.875672e-02 7 105143084 105143330 247 - 2.033 1.918 -0.385
ENSG00000135250 E024 0.4847352 0.2547219826 6.915072e-01 7.929660e-01 7 105143331 105144066 736 - 0.134 0.201 0.709
ENSG00000135250 E025 34.7086220 0.0011143033 8.432808e-02 1.663477e-01 7 105145283 105145308 26 - 1.528 1.420 -0.372
ENSG00000135250 E026 104.9022135 0.0002741904 2.503230e-06 1.906756e-05 7 105146493 105146658 166 - 2.016 1.833 -0.615
ENSG00000135250 E027 0.4439371 0.0215569085 4.635973e-01 6.033740e-01 7 105160301 105160506 206 - 0.187 0.000 -11.408
ENSG00000135250 E028 79.7000747 0.0003434442 1.672655e-07 1.627694e-06 7 105160507 105160544 38 - 1.910 1.666 -0.825
ENSG00000135250 E029 81.2248680 0.0005804375 2.383059e-08 2.737137e-07 7 105160545 105160613 69 - 1.920 1.655 -0.898
ENSG00000135250 E030 84.2548300 0.0004250402 2.195487e-07 2.088204e-06 7 105167377 105167464 88 - 1.932 1.698 -0.793
ENSG00000135250 E031 75.2443990 0.0003725103 1.638839e-04 8.087345e-04 7 105168008 105168095 88 - 1.870 1.698 -0.581
ENSG00000135250 E032 69.4993178 0.0003775116 3.329401e-04 1.512034e-03 7 105169157 105169265 109 - 1.836 1.666 -0.574
ENSG00000135250 E033 0.1451727 0.0435584748 1.000000e+00   7 105203627 105203627 1 - 0.072 0.000 -9.826
ENSG00000135250 E034 46.1621719 0.0004666428 1.299574e-01 2.341087e-01 7 105203628 105203693 66 - 1.644 1.567 -0.261
ENSG00000135250 E035 23.5932262 0.0008091467 6.789822e-01 7.832951e-01 7 105203694 105203722 29 - 1.333 1.379 0.161
ENSG00000135250 E036 24.1840628 0.0010613789 6.460453e-01 7.580664e-01 7 105203723 105203785 63 - 1.358 1.334 -0.081
ENSG00000135250 E037 0.1515154 0.0432939189 1.000000e+00   7 105206401 105206529 129 - 0.072 0.000 -9.827
ENSG00000135250 E038 4.5447981 0.0036552202 6.741165e-01 7.796331e-01 7 105268806 105268869 64 - 0.711 0.656 -0.229
ENSG00000135250 E039 1.7736722 0.0079114806 2.033463e-02 5.218552e-02 7 105268870 105269015 146 - 0.500 0.000 -13.310
ENSG00000135250 E040 0.8878717 0.0175704498 9.082347e-02 1.764344e-01 7 105297421 105297533 113 - 0.134 0.442 2.296
ENSG00000135250 E041 0.1451727 0.0435584748 1.000000e+00   7 105298738 105298779 42 - 0.072 0.000 -9.826
ENSG00000135250 E042 2.8817666 0.0056757090 1.911267e-02 4.958488e-02 7 105306436 105306571 136 - 0.418 0.799 1.708
ENSG00000135250 E043 29.7064669 0.0006976453 2.982323e-02 7.161681e-02 7 105388648 105388702 55 - 1.474 1.322 -0.525
ENSG00000135250 E044 25.5600915 0.0011494065 2.089306e-03 7.500147e-03 7 105388791 105388939 149 - 1.430 1.180 -0.876
ENSG00000135250 E045 0.8899506 0.0134973553 1.437888e-01 2.531679e-01 7 105389244 105389391 148 - 0.318 0.000 -12.373
ENSG00000135250 E046 0.0000000       7 105399156 105399308 153 -