ENSG00000135249

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000257700 ENSG00000135249 HEK293_OSMI2_2hA HEK293_TMG_2hB RINT1 protein_coding protein_coding 16.68641 4.766592 27.02501 0.4318309 0.2397537 2.500775 12.3536732 2.6663457 21.5101040 0.2383857 0.28446976 3.0073487 0.6718458 0.56866667 0.79596667 0.22730000 0.008817295 0.008817295 FALSE TRUE
ENST00000497979 ENSG00000135249 HEK293_OSMI2_2hA HEK293_TMG_2hB RINT1 protein_coding nonsense_mediated_decay 16.68641 4.766592 27.02501 0.4318309 0.2397537 2.500775 0.2092258 1.1173806 0.0000000 0.5597521 0.00000000 -6.8168309 0.0397125 0.21556667 0.00000000 -0.21556667 0.037868029 0.008817295 FALSE TRUE
MSTRG.30462.1 ENSG00000135249 HEK293_OSMI2_2hA HEK293_TMG_2hB RINT1 protein_coding   16.68641 4.766592 27.02501 0.4318309 0.2397537 2.500775 1.2309683 0.3961137 2.1846287 0.2598366 1.31117015 2.4340214 0.1046125 0.07793333 0.08013333 0.00220000 1.000000000 0.008817295 FALSE TRUE
MSTRG.30462.7 ENSG00000135249 HEK293_OSMI2_2hA HEK293_TMG_2hB RINT1 protein_coding   16.68641 4.766592 27.02501 0.4318309 0.2397537 2.500775 0.3842633 0.2955338 0.1864076 0.2383345 0.09340235 -0.6374812 0.0444750 0.07243333 0.00690000 -0.06553333 0.627609202 0.008817295 FALSE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000135249 E001 0.2987644 0.0276273340 1.000000e+00   7 105532169 105532180 12 + 0.108 0.000 -9.611
ENSG00000135249 E002 0.4439371 0.0215896156 1.000000e+00 1.000000e+00 7 105532181 105532183 3 + 0.153 0.000 -10.189
ENSG00000135249 E003 7.3297474 0.0022692335 2.700787e-01 4.086546e-01 7 105532184 105532200 17 + 0.850 0.677 -0.697
ENSG00000135249 E004 7.9240541 0.0020842255 3.645681e-01 5.089222e-01 7 105532201 105532206 6 + 0.875 0.740 -0.532
ENSG00000135249 E005 10.7714696 0.0015629906 4.354240e-01 5.776635e-01 7 105532207 105532211 5 + 0.990 0.889 -0.378
ENSG00000135249 E006 70.5838061 0.0003976611 1.719744e-05 1.082681e-04 7 105532212 105532357 146 + 1.786 1.521 -0.898
ENSG00000135249 E007 67.5406630 0.0004055970 3.002128e-04 1.380413e-03 7 105532824 105532869 46 + 1.762 1.541 -0.752
ENSG00000135249 E008 0.7320925 0.0654468689 4.602771e-01 6.003188e-01 7 105535572 105535691 120 + 0.232 0.000 -10.924
ENSG00000135249 E009 58.6121827 0.0004385814 2.077211e-04 9.970141e-04 7 105536565 105536612 48 + 1.705 1.458 -0.843
ENSG00000135249 E010 50.3050518 0.0004678693 9.691185e-05 5.075936e-04 7 105536613 105536657 45 + 1.643 1.357 -0.983
ENSG00000135249 E011 69.3227108 0.0003465743 8.160357e-06 5.531937e-05 7 105536658 105536749 92 + 1.780 1.501 -0.946
ENSG00000135249 E012 1.4372407 0.0096437118 2.041624e-01 3.313739e-01 7 105541601 105541855 255 + 0.267 0.512 1.407
ENSG00000135249 E013 1.4821915 0.0420741089 5.251365e-02 1.136367e-01 7 105542240 105542407 168 + 0.232 0.602 2.086
ENSG00000135249 E014 58.4125334 0.0008495790 6.867319e-03 2.088137e-02 7 105542408 105542491 84 + 1.696 1.521 -0.594
ENSG00000135249 E015 108.3785849 0.0003644432 1.952248e-05 1.214088e-04 7 105542492 105542649 158 + 1.962 1.757 -0.691
ENSG00000135249 E016 127.6206297 0.0003688477 1.840057e-02 4.801165e-02 7 105546910 105547083 174 + 2.018 1.921 -0.324
ENSG00000135249 E017 109.7731629 0.0002634841 3.822603e-02 8.777310e-02 7 105547184 105547333 150 + 1.953 1.863 -0.303
ENSG00000135249 E018 45.0567404 0.0005803146 2.981759e-01 4.395892e-01 7 105548554 105548555 2 + 1.570 1.501 -0.236
ENSG00000135249 E019 113.7756127 0.0002579328 3.120115e-01 4.545357e-01 7 105548556 105548710 155 + 1.961 1.921 -0.134
ENSG00000135249 E020 39.7625937 0.0005765004 6.052811e-01 7.257090e-01 7 105550055 105550058 4 + 1.514 1.480 -0.116
ENSG00000135249 E021 89.5677575 0.0002846788 4.142359e-01 5.576413e-01 7 105550059 105550165 107 + 1.857 1.821 -0.122
ENSG00000135249 E022 161.5407253 0.0002361598 5.730316e-01 6.990329e-01 7 105550261 105550486 226 + 2.107 2.090 -0.055
ENSG00000135249 E023 133.0083967 0.0002482968 9.620300e-01 9.800536e-01 7 105551570 105551707 138 + 2.020 2.022 0.006
ENSG00000135249 E024 152.6387853 0.0006824227 6.646326e-01 7.724336e-01 7 105555028 105555227 200 + 2.076 2.095 0.065
ENSG00000135249 E025 0.0000000       7 105555228 105555363 136 +      
ENSG00000135249 E026 146.9446865 0.0025603057 8.907232e-02 1.737115e-01 7 105563733 105563947 215 + 2.048 2.119 0.239
ENSG00000135249 E027 104.6681510 0.0003279971 1.861496e-04 9.042200e-04 7 105565277 105565457 181 + 1.885 2.043 0.530
ENSG00000135249 E028 78.3369007 0.0014718448 3.636542e-07 3.306926e-06 7 105565530 105565648 119 + 1.735 1.986 0.847
ENSG00000135249 E029 195.2069752 0.0002445668 2.743155e-35 6.819979e-33 7 105567119 105567677 559 + 2.098 2.444 1.156