ENSG00000135148

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000257604 ENSG00000135148 HEK293_OSMI2_2hA HEK293_TMG_2hB TRAFD1 protein_coding protein_coding 44.75259 56.53436 37.12873 4.810424 1.652801 -0.6064589 5.210490 6.3822903 5.283380 0.8674893 0.4307378 -0.2721438 0.11907083 0.1146333 0.1423667 0.02773333 5.868359e-01 5.160422e-23 FALSE TRUE
ENST00000412615 ENSG00000135148 HEK293_OSMI2_2hA HEK293_TMG_2hB TRAFD1 protein_coding protein_coding 44.75259 56.53436 37.12873 4.810424 1.652801 -0.6064589 32.498022 44.8059569 24.217176 5.0116857 0.8762199 -0.8873862 0.71529167 0.7891333 0.6528000 -0.13633333 7.328039e-03 5.160422e-23 FALSE TRUE
ENST00000550051 ENSG00000135148 HEK293_OSMI2_2hA HEK293_TMG_2hB TRAFD1 protein_coding retained_intron 44.75259 56.53436 37.12873 4.810424 1.652801 -0.6064589 2.642120 0.8629649 3.503646 0.1622805 0.8638114 2.0089732 0.06452083 0.0160000 0.0929000 0.07690000 2.241668e-04 5.160422e-23 FALSE TRUE
MSTRG.8047.2 ENSG00000135148 HEK293_OSMI2_2hA HEK293_TMG_2hB TRAFD1 protein_coding   44.75259 56.53436 37.12873 4.810424 1.652801 -0.6064589 1.198005 3.1700598 0.000000 0.1220952 0.0000000 -8.3129101 0.02368750 0.0573000 0.0000000 -0.05730000 5.160422e-23 5.160422e-23 FALSE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000135148 E001 0.0000000       12 112125515 112125516 2 +      
ENSG00000135148 E002 2.5692841 0.0714181159 7.306651e-01 8.225360e-01 12 112125517 112125537 21 + 0.558 0.507 -0.239
ENSG00000135148 E003 4.1484608 0.0568375899 6.123917e-01 7.313424e-01 12 112125538 112125544 7 + 0.728 0.649 -0.330
ENSG00000135148 E004 9.3060089 0.0094236730 3.731092e-02 8.605569e-02 12 112125545 112125553 9 + 1.120 0.894 -0.834
ENSG00000135148 E005 14.4030879 0.0093904261 2.707883e-02 6.615262e-02 12 112125554 112125556 3 + 1.285 1.078 -0.735
ENSG00000135148 E006 19.6293574 0.0010620738 7.900810e-03 2.352872e-02 12 112125557 112125559 3 + 1.404 1.213 -0.668
ENSG00000135148 E007 25.5661519 0.0008261388 1.627388e-04 8.037537e-04 12 112125560 112125562 3 + 1.543 1.301 -0.838
ENSG00000135148 E008 155.8036927 0.0016754344 1.841628e-02 4.804600e-02 12 112125563 112125618 56 + 2.212 2.144 -0.228
ENSG00000135148 E009 89.3176377 0.0112033845 5.447985e-02 1.170890e-01 12 112125619 112126084 466 + 2.000 1.885 -0.387
ENSG00000135148 E010 49.5147426 0.0189825812 2.256072e-01 3.571211e-01 12 112126085 112126149 65 + 1.733 1.640 -0.315
ENSG00000135148 E011 1.2490919 0.0474203412 4.072278e-01 5.508139e-01 12 112130470 112130510 41 + 0.438 0.294 -0.844
ENSG00000135148 E012 233.3961073 0.0059540512 1.358206e-01 2.422383e-01 12 112130511 112130569 59 + 2.377 2.321 -0.188
ENSG00000135148 E013 0.3697384 0.0250036985 4.240390e-01 5.670346e-01 12 112133140 112133320 181 + 0.000 0.172 9.429
ENSG00000135148 E014 349.6529691 0.0026494273 2.748486e-02 6.697698e-02 12 112134738 112134873 136 + 2.553 2.496 -0.191
ENSG00000135148 E015 247.3340865 0.0002222128 7.699019e-04 3.149549e-03 12 112135013 112135066 54 + 2.410 2.347 -0.209
ENSG00000135148 E016 1.4037289 0.1456444675 1.136048e-01 2.107880e-01 12 112140119 112140205 87 + 0.558 0.237 -1.846
ENSG00000135148 E017 380.5398369 0.0001402230 2.639707e-05 1.591309e-04 12 112140819 112140980 162 + 2.596 2.534 -0.207
ENSG00000135148 E018 516.7101791 0.0004941535 3.804357e-03 1.259369e-02 12 112140981 112141224 244 + 2.712 2.674 -0.128
ENSG00000135148 E019 8.9624552 0.0611425067 1.289826e-03 4.942030e-03 12 112141225 112141491 267 + 1.245 0.733 -1.909
ENSG00000135148 E020 7.9090212 0.0021800782 4.048264e-05 2.330107e-04 12 112141953 112142088 136 + 1.174 0.731 -1.669
ENSG00000135148 E021 429.8765928 0.0019080204 8.233838e-01 8.887132e-01 12 112142089 112142295 207 + 2.601 2.608 0.023
ENSG00000135148 E022 300.3720890 0.0027210025 3.680518e-01 5.124550e-01 12 112145586 112145662 77 + 2.461 2.446 -0.052
ENSG00000135148 E023 560.8182830 0.0008257757 1.500969e-01 2.618051e-01 12 112148074 112148304 231 + 2.731 2.719 -0.041
ENSG00000135148 E024 181.3429583 0.0003013145 1.128429e-03 4.399349e-03 12 112149751 112149756 6 + 2.283 2.211 -0.243
ENSG00000135148 E025 386.4277538 0.0007143457 5.125181e-01 6.474379e-01 12 112149757 112149871 115 + 2.560 2.563 0.009
ENSG00000135148 E026 653.8803562 0.0015029850 5.943042e-03 1.845875e-02 12 112151801 112152140 340 + 2.734 2.811 0.256
ENSG00000135148 E027 304.8717075 0.0001912728 1.364654e-05 8.784858e-05 12 112152427 112152499 73 + 2.379 2.494 0.381
ENSG00000135148 E028 1.1468953 0.0113410015 2.383685e-01 3.722515e-01 12 112152500 112152504 5 + 0.438 0.238 -1.253
ENSG00000135148 E029 197.2401394 0.0002750715 7.202085e-04 2.971348e-03 12 112152735 112152740 6 + 2.194 2.306 0.373
ENSG00000135148 E030 1084.5906881 0.0020164000 7.231887e-11 1.306436e-09 12 112152741 112153604 864 + 2.894 3.050 0.518