ENSG00000135108

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000330622 ENSG00000135108 HEK293_OSMI2_2hA HEK293_TMG_2hB FBXO21 protein_coding protein_coding 18.1164 10.21763 26.66193 1.974063 0.2812056 1.38285 6.286632 1.931348 10.354329 0.4973983 0.3031522 2.4164958 0.3145375 0.1931000 0.3882333 0.1951333 2.766929e-02 4.488406e-21 FALSE TRUE
ENST00000622495 ENSG00000135108 HEK293_OSMI2_2hA HEK293_TMG_2hB FBXO21 protein_coding protein_coding 18.1164 10.21763 26.66193 1.974063 0.2812056 1.38285 8.656010 7.850658 10.542075 1.8241660 0.4535064 0.4248047 0.5513375 0.7603667 0.3952000 -0.3651667 5.550257e-07 4.488406e-21 FALSE TRUE
MSTRG.8106.4 ENSG00000135108 HEK293_OSMI2_2hA HEK293_TMG_2hB FBXO21 protein_coding   18.1164 10.21763 26.66193 1.974063 0.2812056 1.38285 2.494021 0.000000 4.101645 0.0000000 0.3898520 8.6835718 0.0916875 0.0000000 0.1540000 0.1540000 4.488406e-21 4.488406e-21 FALSE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000135108 E001 765.5458052 0.0026493022 2.443430e-22 2.023166e-20 12 117141991 117145048 3058 - 2.758 3.017 0.860
ENSG00000135108 E002 65.8732734 0.0004129719 1.458592e-02 3.955932e-02 12 117145049 117145067 19 - 1.756 1.908 0.512
ENSG00000135108 E003 416.4325638 0.0005298401 3.819284e-05 2.212359e-04 12 117145068 117145915 848 - 2.565 2.688 0.408
ENSG00000135108 E004 252.1564249 0.0001953063 2.557096e-01 3.926264e-01 12 117145916 117146277 362 - 2.391 2.412 0.071
ENSG00000135108 E005 0.4481018 0.0279169032 3.047564e-01 4.467864e-01 12 117154250 117154302 53 - 0.091 0.279 1.942
ENSG00000135108 E006 4.5693912 0.0039138613 9.892495e-05 5.168087e-04 12 117155054 117155615 562 - 0.422 1.001 2.461
ENSG00000135108 E007 154.3871111 0.0002120454 2.900279e-02 7.001226e-02 12 117155791 117155948 158 - 2.193 2.176 -0.057
ENSG00000135108 E008 158.5793751 0.0002197179 1.708688e-04 8.388801e-04 12 117157873 117158063 191 - 2.222 2.160 -0.208
ENSG00000135108 E009 25.7142977 0.0007427463 5.378723e-03 1.695790e-02 12 117158064 117158084 21 - 1.475 1.320 -0.537
ENSG00000135108 E010 147.3620622 0.0002370027 6.099369e-03 1.887097e-02 12 117165485 117165617 133 - 2.178 2.142 -0.121
ENSG00000135108 E011 161.4297400 0.0006381309 4.396459e-03 1.425435e-02 12 117166898 117167077 180 - 2.220 2.179 -0.138
ENSG00000135108 E012 69.3038986 0.0024371002 6.848547e-04 2.843821e-03 12 117172471 117172509 39 - 1.884 1.755 -0.434
ENSG00000135108 E013 111.7708934 0.0003147993 3.172005e-07 2.920239e-06 12 117172510 117172607 98 - 2.093 1.958 -0.451
ENSG00000135108 E014 137.3936542 0.0002907506 1.466797e-07 1.442710e-06 12 117174205 117174341 137 - 2.178 2.058 -0.403
ENSG00000135108 E015 60.7571262 0.0064576779 1.405931e-01 2.488410e-01 12 117174651 117174654 4 - 1.802 1.761 -0.138
ENSG00000135108 E016 153.0547942 0.0021743279 9.846877e-06 6.556982e-05 12 117174655 117174797 143 - 2.224 2.101 -0.410
ENSG00000135108 E017 131.9837268 0.0011036053 8.801563e-08 9.030217e-07 12 117177520 117177641 122 - 2.168 2.020 -0.497
ENSG00000135108 E018 0.0000000       12 117184785 117184810 26 -      
ENSG00000135108 E019 104.1923220 0.0003262089 8.260395e-09 1.034102e-07 12 117186477 117186571 95 - 2.073 1.906 -0.561
ENSG00000135108 E020 0.8942170 0.0135712173 4.124567e-02 9.333446e-02 12 117188759 117188793 35 - 0.381 0.000 -11.704
ENSG00000135108 E021 0.5954526 0.0172671820 1.228670e-01 2.240458e-01 12 117188794 117189226 433 - 0.287 0.000 -11.119
ENSG00000135108 E022 111.5476078 0.0003026978 4.173898e-05 2.395143e-04 12 117189227 117189362 136 - 2.079 1.981 -0.329
ENSG00000135108 E023 97.8620825 0.0102378932 2.665942e-02 6.528292e-02 12 117190218 117190470 253 - 2.025 1.911 -0.382