ENSG00000135097

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000257552 ENSG00000135097 HEK293_OSMI2_2hA HEK293_TMG_2hB MSI1 protein_coding protein_coding 13.53729 17.61864 15.10873 0.7851206 0.5230914 -0.2215837 9.9210889 15.8951716 8.994607 0.4478296 0.1034441 -0.8207608 0.72648750 0.903566667 0.5971667 -0.3064000 5.390599e-09 5.390599e-09 FALSE TRUE
ENST00000546985 ENSG00000135097 HEK293_OSMI2_2hA HEK293_TMG_2hB MSI1 protein_coding protein_coding 13.53729 17.61864 15.10873 0.7851206 0.5230914 -0.2215837 0.6926811 0.5117250 0.000000 0.4113875 0.0000000 -5.7052178 0.05389583 0.027300000 0.0000000 -0.0273000 2.542102e-01 5.390599e-09 FALSE FALSE
MSTRG.8147.3 ENSG00000135097 HEK293_OSMI2_2hA HEK293_TMG_2hB MSI1 protein_coding   13.53729 17.61864 15.10873 0.7851206 0.5230914 -0.2215837 1.6265118 1.0923097 2.764982 0.1361472 0.3742792 1.3319487 0.12020833 0.061833333 0.1818667 0.1200333 1.311331e-05 5.390599e-09 FALSE TRUE
MSTRG.8147.4 ENSG00000135097 HEK293_OSMI2_2hA HEK293_TMG_2hB MSI1 protein_coding   13.53729 17.61864 15.10873 0.7851206 0.5230914 -0.2215837 1.1923721 0.1194303 3.268191 0.1194303 0.3452048 4.6626527 0.08859167 0.007366667 0.2155667 0.2082000 1.855109e-03 5.390599e-09 FALSE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000135097 E001 296.8820019 0.0105073382 5.729842e-12 1.240560e-10 12 120341330 120341838 509 - 2.183 2.589 1.353
ENSG00000135097 E002 448.5433870 0.0031755270 3.835590e-15 1.343889e-13 12 120341839 120342881 1043 - 2.459 2.736 0.922
ENSG00000135097 E003 53.9093650 0.0004581449 5.507726e-01 6.803389e-01 12 120342882 120343105 224 - 1.682 1.761 0.267
ENSG00000135097 E004 67.1292406 0.0020430910 8.346929e-01 8.965255e-01 12 120345570 120345632 63 - 1.800 1.840 0.134
ENSG00000135097 E005 81.0000793 0.0005880042 1.843970e-01 3.067487e-01 12 120346135 120346221 87 - 1.905 1.902 -0.011
ENSG00000135097 E006 78.6882814 0.0030987465 4.540476e-01 5.947285e-01 12 120346222 120346289 68 - 1.883 1.898 0.049
ENSG00000135097 E007 46.1016592 0.0230492614 5.227302e-01 6.564777e-01 12 120346290 120346322 33 - 1.664 1.660 -0.015
ENSG00000135097 E008 88.7498358 0.0022964781 1.643027e-02 4.368040e-02 12 120347446 120347514 69 - 1.973 1.920 -0.178
ENSG00000135097 E009 84.4292100 0.0003621592 7.064956e-03 2.139373e-02 12 120351344 120351400 57 - 1.955 1.902 -0.178
ENSG00000135097 E010 82.7243202 0.0004163349 7.383309e-02 1.496449e-01 12 120353299 120353322 24 - 1.926 1.906 -0.066
ENSG00000135097 E011 95.4852432 0.0002900488 6.234815e-02 1.305979e-01 12 120353323 120353379 57 - 1.983 1.966 -0.058
ENSG00000135097 E012 133.8863022 0.0002537407 2.701182e-02 6.602298e-02 12 120356902 120357019 118 - 2.128 2.111 -0.058
ENSG00000135097 E013 132.6485654 0.0024661656 3.245004e-02 7.671796e-02 12 120357816 120357898 83 - 2.131 2.102 -0.099
ENSG00000135097 E014 113.8189618 0.0004547421 1.144842e-04 5.884231e-04 12 120359005 120359053 49 - 2.092 2.012 -0.266
ENSG00000135097 E015 0.2987644 0.0294119566 1.286582e-01   12 120361394 120361508 115 - 0.235 0.000 -10.237
ENSG00000135097 E016 126.5237617 0.0011648556 5.294068e-05 2.964750e-04 12 120363043 120363135 93 - 2.142 2.054 -0.294
ENSG00000135097 E017 84.2500067 0.0030296870 4.564004e-05 2.596923e-04 12 120364714 120364755 42 - 1.991 1.857 -0.451
ENSG00000135097 E018 91.1490104 0.0076182064 1.121810e-02 3.168966e-02 12 120368008 120368092 85 - 1.999 1.915 -0.284
ENSG00000135097 E019 82.0501800 0.0068380310 5.839636e-05 3.234800e-04 12 120368192 120368273 82 - 1.998 1.831 -0.559
ENSG00000135097 E020 55.1484115 0.0027063187 3.568587e-10 5.738545e-09 12 120368833 120368873 41 - 1.879 1.600 -0.945
ENSG00000135097 E021 50.0022898 0.0115950340 8.409855e-11 1.503323e-09 12 120369033 120369227 195 - 1.893 1.477 -1.411