ENSG00000135018

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000257468 ENSG00000135018 HEK293_OSMI2_2hA HEK293_TMG_2hB UBQLN1 protein_coding protein_coding 51.4529 28.92695 76.49424 1.899415 0.9332993 1.402627 9.066040 3.361946 14.240095 0.7022933 1.16247040 2.079318 0.16358750 0.11916667 0.186600000 0.06743333 3.016198e-01 1.941473e-05 FALSE  
ENST00000376395 ENSG00000135018 HEK293_OSMI2_2hA HEK293_TMG_2hB UBQLN1 protein_coding protein_coding 51.4529 28.92695 76.49424 1.899415 0.9332993 1.402627 39.110445 22.785072 59.386890 1.3657929 1.80790409 1.381665 0.76247500 0.78830000 0.776000000 -0.01230000 8.526089e-01 1.941473e-05 FALSE  
MSTRG.32911.3 ENSG00000135018 HEK293_OSMI2_2hA HEK293_TMG_2hB UBQLN1 protein_coding   51.4529 28.92695 76.49424 1.899415 0.9332993 1.402627 1.558597 2.047467 0.763995 0.7042110 0.07545223 -1.410473 0.04072083 0.06843333 0.009966667 -0.05846667 1.941473e-05 1.941473e-05 FALSE  
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000135018 E001 2.135841 0.0072438620 9.070283e-06 6.087329e-05 9 83659968 83659970 3 - 0.082 0.818 4.741
ENSG00000135018 E002 30.656090 0.0141832117 9.344936e-11 1.657029e-09 9 83659971 83660032 62 - 1.200 1.772 1.971
ENSG00000135018 E003 1573.142192 0.0071546193 8.319271e-04 3.373057e-03 9 83660033 83661428 1396 - 3.120 3.260 0.465
ENSG00000135018 E004 779.618252 0.0001319762 2.545669e-15 9.130430e-14 9 83661429 83661901 473 - 2.821 2.948 0.423
ENSG00000135018 E005 243.719356 0.0001961842 1.311147e-01 2.357072e-01 9 83661902 83661910 9 - 2.345 2.402 0.192
ENSG00000135018 E006 279.365382 0.0001594192 2.082660e-01 3.364002e-01 9 83661911 83661939 29 - 2.406 2.456 0.165
ENSG00000135018 E007 547.155778 0.0011716752 6.176969e-02 1.295983e-01 9 83663875 83664043 169 - 2.693 2.752 0.198
ENSG00000135018 E008 522.964581 0.0001995883 3.303551e-01 4.739512e-01 9 83665030 83665145 116 - 2.693 2.697 0.014
ENSG00000135018 E009 2.888186 0.0054984377 8.270708e-01 8.912442e-01 9 83665146 83665393 248 - 0.570 0.549 -0.096
ENSG00000135018 E010 322.145327 0.0001589569 7.834704e-04 3.200425e-03 9 83666350 83666394 45 - 2.502 2.450 -0.172
ENSG00000135018 E011 285.479380 0.0001617263 6.228798e-05 3.427250e-04 9 83666395 83666433 39 - 2.456 2.385 -0.238
ENSG00000135018 E012 52.313701 0.0004528404 1.998624e-07 1.914707e-06 9 83666434 83669184 2751 - 1.774 1.498 -0.941
ENSG00000135018 E013 520.048679 0.0005439606 2.148064e-01 3.442146e-01 9 83669185 83669327 143 - 2.694 2.691 -0.010
ENSG00000135018 E014 230.631165 0.0002093080 3.527073e-01 4.969422e-01 9 83677727 83677751 25 - 2.341 2.339 -0.008
ENSG00000135018 E015 516.516434 0.0001539104 7.966217e-01 8.702132e-01 9 83677752 83677961 210 - 2.682 2.707 0.085
ENSG00000135018 E016 481.951136 0.0013789499 2.073806e-02 5.303333e-02 9 83678441 83678599 159 - 2.671 2.632 -0.129
ENSG00000135018 E017 634.496707 0.0011206913 4.951443e-10 7.755569e-09 9 83679775 83680037 263 - 2.813 2.696 -0.390
ENSG00000135018 E018 386.038298 0.0010525861 8.517063e-07 7.181467e-06 9 83682951 83683066 116 - 2.594 2.489 -0.348
ENSG00000135018 E019 386.190837 0.0003479558 2.798984e-14 8.613696e-13 9 83686004 83686155 152 - 2.605 2.460 -0.481
ENSG00000135018 E020 404.785614 0.0086048113 4.193983e-03 1.368854e-02 9 83707500 83707958 459 - 2.620 2.497 -0.410