ENSG00000135002

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000376736 ENSG00000135002 HEK293_OSMI2_2hA HEK293_TMG_2hB RFK protein_coding protein_coding 16.15091 5.143398 30.98872 1.019677 2.328655 2.588613 11.732297 5.095187 21.853118 1.033229 1.287741 2.098463 0.8333042 0.9890667 0.7075000 -0.2815667 1.796544e-19 1.796544e-19 FALSE TRUE
ENST00000476087 ENSG00000135002 HEK293_OSMI2_2hA HEK293_TMG_2hB RFK protein_coding processed_transcript 16.15091 5.143398 30.98872 1.019677 2.328655 2.588613 3.820732 0.000000 8.431102 0.000000 1.377097 9.721288 0.1304958 0.0000000 0.2683333 0.2683333 2.170832e-19 1.796544e-19   FALSE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000135002 E001 2.0949560 0.0089705603 7.297227e-07 6.243227e-06 9 76385526 76385545 20 - 0.118 0.928 4.587
ENSG00000135002 E002 456.7272921 0.0038674580 2.902358e-08 3.276191e-07 9 76385546 76386980 1435 - 2.537 2.706 0.563
ENSG00000135002 E003 190.3373255 0.0002093729 8.088250e-03 2.400353e-02 9 76386981 76387283 303 - 2.177 2.282 0.353
ENSG00000135002 E004 77.9451181 0.0015920698 4.889485e-01 6.264529e-01 9 76387284 76387307 24 - 1.802 1.864 0.211
ENSG00000135002 E005 202.7365962 0.0003319299 4.684036e-02 1.035469e-01 9 76387308 76387529 222 - 2.235 2.207 -0.094
ENSG00000135002 E006 3.9930392 0.1007737266 9.572452e-01 9.772074e-01 9 76387584 76388182 599 - 0.630 0.643 0.058
ENSG00000135002 E007 1.0308569 0.0361119601 1.823282e-01 3.041350e-01 9 76388183 76388286 104 - 0.320 0.000 -11.135
ENSG00000135002 E008 1.7286183 0.0080592812 9.060216e-01 9.442507e-01 9 76388287 76388553 267 - 0.380 0.374 -0.037
ENSG00000135002 E009 154.3478392 0.0002458899 1.007031e-05 6.686073e-05 9 76388554 76388656 103 - 2.134 2.008 -0.424
ENSG00000135002 E010 146.5510216 0.0008076179 2.243101e-09 3.115232e-08 9 76392418 76392569 152 - 2.124 1.914 -0.705
ENSG00000135002 E011 0.4375944 0.0255501705 6.777707e-01 7.823976e-01 9 76393714 76393799 86 - 0.166 0.000 -9.912
ENSG00000135002 E012 0.8878743 0.0131971975 2.362657e-01 3.698456e-01 9 76393800 76394089 290 - 0.286 0.000 -10.913
ENSG00000135002 E013 122.0144790 0.0135005291 4.220102e-05 2.419092e-04 9 76394090 76394517 428 - 2.054 1.775 -0.937