ENSG00000134987

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000505303 ENSG00000134987 HEK293_OSMI2_2hA HEK293_TMG_2hB WDR36 protein_coding retained_intron 13.45119 3.285845 21.94247 0.5767143 1.140799 2.735663 1.8657260 0.04600512 2.615555 0.04600512 0.2026363 5.550920 0.08101667 0.01530000 0.1196000 1.043000e-01 0.001822412 0.0001488585 FALSE TRUE
ENST00000513710 ENSG00000134987 HEK293_OSMI2_2hA HEK293_TMG_2hB WDR36 protein_coding protein_coding 13.45119 3.285845 21.94247 0.5767143 1.140799 2.735663 9.8259271 2.87974710 16.114181 0.24778324 0.4135837 2.480211 0.77213333 0.90276667 0.7372333 -1.655333e-01 0.268580184 0.0001488585 FALSE TRUE
MSTRG.26730.1 ENSG00000134987 HEK293_OSMI2_2hA HEK293_TMG_2hB WDR36 protein_coding   13.45119 3.285845 21.94247 0.5767143 1.140799 2.735663 0.8916945 0.36009235 1.829356 0.36009235 0.4453067 2.313244 0.09343750 0.08193333 0.0820000 6.666667e-05 0.583274314 0.0001488585 FALSE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000134987 E001 9.5578668 0.0018096915 4.002843e-02 9.108796e-02 5 111092321 111092346 26 + 0.875 0.691 -0.732
ENSG00000134987 E002 9.8545549 0.0016716085 3.226515e-02 7.636184e-02 5 111092347 111092347 1 + 0.887 0.691 -0.779
ENSG00000134987 E003 77.4426590 0.0003215210 2.540860e-05 1.538132e-04 5 111092348 111092618 271 + 1.717 1.634 -0.281
ENSG00000134987 E004 28.3096460 0.0007054097 2.435923e-02 6.055988e-02 5 111094920 111094947 28 + 1.293 1.246 -0.164
ENSG00000134987 E005 4.7403259 0.0033336500 3.174642e-03 1.078076e-02 5 111094948 111095209 262 + 0.650 0.000 -13.845
ENSG00000134987 E006 0.7320925 0.0994798187 5.565348e-01 6.851559e-01 5 111097007 111097078 72 + 0.188 0.000 -11.165
ENSG00000134987 E007 55.0878262 0.0004592232 5.823597e-04 2.468647e-03 5 111097079 111097179 101 + 1.570 1.496 -0.254
ENSG00000134987 E008 69.9779766 0.0003569722 4.691943e-04 2.042263e-03 5 111098722 111098839 118 + 1.669 1.624 -0.154
ENSG00000134987 E009 68.4754191 0.0003788577 1.118842e-02 3.161665e-02 5 111100589 111100721 133 + 1.652 1.672 0.068
ENSG00000134987 E010 44.7633904 0.0005100845 2.698575e-01 4.084080e-01 5 111102345 111102399 55 + 1.463 1.559 0.328
ENSG00000134987 E011 75.5573565 0.0003449691 4.653480e-03 1.496779e-02 5 111103786 111103918 133 + 1.697 1.707 0.034
ENSG00000134987 E012 98.6264256 0.0058710418 3.508947e-03 1.175213e-02 5 111104177 111104352 176 + 1.813 1.796 -0.057
ENSG00000134987 E013 93.3899373 0.0107304131 7.266821e-03 2.191813e-02 5 111104697 111104817 121 + 1.792 1.760 -0.109
ENSG00000134987 E014 68.4777728 0.0005024652 1.892131e-05 1.180072e-04 5 111105295 111105360 66 + 1.668 1.559 -0.373
ENSG00000134987 E015 72.0079403 0.0015907127 1.964208e-08 2.294163e-07 5 111106057 111106143 87 + 1.699 1.468 -0.791
ENSG00000134987 E016 96.2912723 0.0003107703 1.268406e-10 2.200914e-09 5 111107294 111107439 146 + 1.821 1.614 -0.701
ENSG00000134987 E017 80.5393944 0.0003040566 1.988975e-05 1.234416e-04 5 111110189 111110303 115 + 1.734 1.653 -0.273
ENSG00000134987 E018 109.0021976 0.0007449740 5.336258e-08 5.717652e-07 5 111110788 111110953 166 + 1.866 1.746 -0.403
ENSG00000134987 E019 91.7231650 0.0060779843 2.727200e-03 9.454324e-03 5 111111170 111111278 109 + 1.783 1.754 -0.099
ENSG00000134987 E020 1.8382889 0.2715736836 4.073148e-01 5.508921e-01 5 111111279 111111552 274 + 0.270 0.610 1.834
ENSG00000134987 E021 69.9283029 0.0003734217 5.823864e-03 1.814690e-02 5 111113074 111113153 80 + 1.664 1.672 0.026
ENSG00000134987 E022 94.3029953 0.0002921267 7.018433e-04 2.905768e-03 5 111119013 111119120 108 + 1.793 1.790 -0.010
ENSG00000134987 E023 94.2664039 0.0006078461 1.052825e-02 3.004286e-02 5 111120496 111120593 98 + 1.787 1.829 0.144
ENSG00000134987 E024 40.9108557 0.0005559942 1.655113e-01 2.823661e-01 5 111120996 111121001 6 + 1.428 1.496 0.235
ENSG00000134987 E025 91.6851609 0.0007847418 1.066839e-01 2.006564e-01 5 111121002 111121141 140 + 1.767 1.860 0.312
ENSG00000134987 E026 88.4464123 0.0002920644 1.259871e-02 3.496612e-02 5 111123805 111123924 120 + 1.759 1.803 0.150
ENSG00000134987 E027 82.7887436 0.0221996611 5.499789e-01 6.796599e-01 5 111124108 111124189 82 + 1.719 1.829 0.371
ENSG00000134987 E028 133.6699846 0.0002473057 3.562873e-02 8.286078e-02 5 111125608 111125795 188 + 1.930 2.020 0.303
ENSG00000134987 E029 950.2107036 0.0204787829 8.690074e-09 1.083311e-07 5 111126734 111130502 3769 + 2.708 3.145 1.453