ENSG00000134709

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000371208 ENSG00000134709 HEK293_OSMI2_2hA HEK293_TMG_2hB HOOK1 protein_coding protein_coding 8.545768 2.091706 16.13833 0.1398265 0.07712192 2.941752 6.82902335 0.5562499 13.044414 0.2971592 0.5278050 4.526955 0.66021667 0.2485000 0.8084667 0.5599667 7.376628e-02 6.182575e-15 FALSE TRUE
ENST00000455990 ENSG00000134709 HEK293_OSMI2_2hA HEK293_TMG_2hB HOOK1 protein_coding protein_coding 8.545768 2.091706 16.13833 0.1398265 0.07712192 2.941752 0.28005149 0.0000000 1.352797 0.0000000 0.8637884 7.090427 0.01617917 0.0000000 0.0834000 0.0834000 4.967906e-01 6.182575e-15 FALSE FALSE
ENST00000491135 ENSG00000134709 HEK293_OSMI2_2hA HEK293_TMG_2hB HOOK1 protein_coding retained_intron 8.545768 2.091706 16.13833 0.1398265 0.07712192 2.941752 0.33139848 1.0754836 0.000000 0.2828786 0.0000000 -6.762194 0.15223333 0.5017667 0.0000000 -0.5017667 6.182575e-15 6.182575e-15   FALSE
MSTRG.1302.2 ENSG00000134709 HEK293_OSMI2_2hA HEK293_TMG_2hB HOOK1 protein_coding   8.545768 2.091706 16.13833 0.1398265 0.07712192 2.941752 0.05675986 0.3692070 0.000000 0.3692070 0.0000000 -5.244914 0.02606250 0.2029000 0.0000000 -0.2029000 5.436682e-01 6.182575e-15 FALSE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000134709 E001 0.1472490 0.0439943332 5.819828e-01   1 59814747 59814773 27 + 0.034 0.000 -16.218
ENSG00000134709 E002 0.1472490 0.0439943332 5.819828e-01   1 59814774 59814785 12 + 0.034 0.000 -16.200
ENSG00000134709 E003 0.2944980 0.4080033079 1.000000e+00   1 59814786 59814810 25 + 0.066 0.000 -17.199
ENSG00000134709 E004 0.4418608 0.0245777460 1.000000e+00 1.000000e+00 1 59814932 59814948 17 + 0.096 0.000 -17.785
ENSG00000134709 E005 10.5040201 0.0015773775 5.750881e-03 1.795789e-02 1 59814949 59815007 59 + 0.831 0.359 -2.168
ENSG00000134709 E006 11.8337552 0.0013966593 2.549667e-03 8.911441e-03 1 59815008 59815014 7 + 0.876 0.359 -2.342
ENSG00000134709 E007 16.2000738 0.0010478215 1.518262e-03 5.695310e-03 1 59815015 59815032 18 + 0.992 0.553 -1.780
ENSG00000134709 E008 13.3838913 0.0012525924 1.028505e-03 4.056864e-03 1 59815033 59815035 3 + 0.921 0.359 -2.514
ENSG00000134709 E009 33.4002423 0.0005992594 1.809745e-02 4.734069e-02 1 59815036 59815171 136 + 1.267 1.180 -0.307
ENSG00000134709 E010 23.2023735 0.0007818167 3.781666e-01 5.224653e-01 1 59815172 59815180 9 + 1.107 1.180 0.261
ENSG00000134709 E011 0.0000000       1 59815181 59815351 171 +      
ENSG00000134709 E012 0.1482932 0.0417954023 2.844833e-02   1 59816632 59816718 87 + 0.000 0.358 23.845
ENSG00000134709 E013 45.3358243 0.0006310866 2.418939e-02 6.020005e-02 1 59821858 59821943 86 + 1.389 1.359 -0.105
ENSG00000134709 E014 0.8900644 0.0473074444 8.019577e-01 8.738100e-01 1 59821944 59822090 147 + 0.175 0.000 -18.785
ENSG00000134709 E015 51.7148065 0.0025820324 3.784547e-04 1.691289e-03 1 59828780 59828852 73 + 1.453 1.279 -0.605
ENSG00000134709 E016 47.8564941 0.0018240675 7.391499e-05 3.989110e-04 1 59832163 59832213 51 + 1.424 1.180 -0.855
ENSG00000134709 E017 67.6822968 0.0004048128 4.841749e-05 2.737993e-04 1 59833405 59833537 133 + 1.566 1.405 -0.552
ENSG00000134709 E018 41.0612546 0.0005002574 3.321246e-05 1.953613e-04 1 59835345 59835412 68 + 1.364 1.052 -1.104
ENSG00000134709 E019 39.9778551 0.0005209104 1.495302e-03 5.620644e-03 1 59836873 59836935 63 + 1.346 1.180 -0.585
ENSG00000134709 E020 49.9858349 0.0016452957 1.283170e-03 4.920608e-03 1 59840308 59840391 84 + 1.439 1.307 -0.458
ENSG00000134709 E021 73.9318163 0.0040771367 7.485816e-03 2.247524e-02 1 59843432 59843598 167 + 1.597 1.567 -0.102
ENSG00000134709 E022 66.5518316 0.0038305570 3.823518e-04 1.706410e-03 1 59847045 59847185 141 + 1.559 1.426 -0.454
ENSG00000134709 E023 81.4120302 0.0003203573 9.514695e-05 4.992329e-04 1 59848315 59848516 202 + 1.642 1.536 -0.360
ENSG00000134709 E024 65.7687493 0.0004107537 2.299352e-04 1.091551e-03 1 59849073 59849183 111 + 1.552 1.426 -0.431
ENSG00000134709 E025 65.9909597 0.0007071010 7.552287e-03 2.265112e-02 1 59858428 59858515 88 + 1.547 1.520 -0.091
ENSG00000134709 E026 51.2865505 0.0013230162 1.548924e-02 4.160484e-02 1 59858985 59859045 61 + 1.441 1.405 -0.125
ENSG00000134709 E027 64.7226526 0.0003971643 1.540685e-03 5.768001e-03 1 59860188 59860282 95 + 1.542 1.466 -0.258
ENSG00000134709 E028 49.7872303 0.0006325771 1.247153e-02 3.466811e-02 1 59860283 59860328 46 + 1.428 1.383 -0.158
ENSG00000134709 E029 6.8692529 0.0158779267 2.285950e-01 3.607564e-01 1 59860329 59862449 2121 + 0.666 0.552 -0.500
ENSG00000134709 E030 61.4170054 0.0019405256 4.909765e-01 6.282948e-01 1 59862784 59862877 94 + 1.503 1.638 0.461
ENSG00000134709 E031 3.3762597 0.0253354099 9.923249e-01 9.992823e-01 1 59863825 59863868 44 + 0.423 0.553 0.642
ENSG00000134709 E032 1.4133945 0.0107451401 2.416058e-01 3.760783e-01 1 59864244 59864631 388 + 0.198 0.553 2.154
ENSG00000134709 E033 42.8037206 0.0005311344 4.106823e-01 5.542912e-01 1 59864632 59864666 35 + 1.354 1.466 0.388
ENSG00000134709 E034 6.6319992 0.0025877393 5.818775e-01 7.063581e-01 1 59864667 59865162 496 + 0.642 0.686 0.189
ENSG00000134709 E035 66.3684682 0.0006284687 3.120615e-01 4.545876e-01 1 59865163 59865245 83 + 1.535 1.650 0.392
ENSG00000134709 E036 67.2497482 0.0003652601 3.447191e-01 4.888210e-01 1 59865872 59865972 101 + 1.542 1.662 0.412
ENSG00000134709 E037 55.9156052 0.0007165190 6.041950e-01 7.248078e-01 1 59868250 59868351 102 + 1.463 1.611 0.507
ENSG00000134709 E038 0.1472490 0.0439943332 5.819828e-01   1 59870877 59871041 165 + 0.034 0.000 -16.200
ENSG00000134709 E039 45.6173927 0.0004564853 7.562830e-01 8.413769e-01 1 59871042 59871110 69 + 1.370 1.583 0.732
ENSG00000134709 E040 630.5081398 0.0194528143 2.289955e-12 5.298304e-11 1 59872795 59876322 3528 + 2.452 2.949 1.654