ENSG00000134698

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000373210 ENSG00000134698 HEK293_OSMI2_2hA HEK293_TMG_2hB AGO4 protein_coding protein_coding 3.467385 1.793003 4.596424 0.04923307 0.4818984 1.353245 1.5460178 0.8919352 2.39746859 0.05804019 0.13430096 1.416421 0.4541083 0.49866667 0.54006667 0.04140000 0.888951754 0.001779863 FALSE TRUE
ENST00000488778 ENSG00000134698 HEK293_OSMI2_2hA HEK293_TMG_2hB AGO4 protein_coding processed_transcript 3.467385 1.793003 4.596424 0.04923307 0.4818984 1.353245 0.5221155 0.1715831 0.39951418 0.17158309 0.20863421 1.173284 0.1424542 0.09183333 0.07880000 -0.01303333 0.801749630 0.001779863 FALSE FALSE
MSTRG.833.1 ENSG00000134698 HEK293_OSMI2_2hA HEK293_TMG_2hB AGO4 protein_coding   3.467385 1.793003 4.596424 0.04923307 0.4818984 1.353245 0.2194587 0.0000000 0.47588624 0.00000000 0.25751291 5.602547 0.0474125 0.00000000 0.09343333 0.09343333 0.359611228 0.001779863 TRUE TRUE
MSTRG.833.3 ENSG00000134698 HEK293_OSMI2_2hA HEK293_TMG_2hB AGO4 protein_coding   3.467385 1.793003 4.596424 0.04923307 0.4818984 1.353245 0.2120067 0.0000000 0.46609333 0.00000000 0.31153649 5.573173 0.0487375 0.00000000 0.11896667 0.11896667 0.410604079 0.001779863 TRUE TRUE
MSTRG.833.4 ENSG00000134698 HEK293_OSMI2_2hA HEK293_TMG_2hB AGO4 protein_coding   3.467385 1.793003 4.596424 0.04923307 0.4818984 1.353245 0.2560474 0.0000000 0.58657918 0.00000000 0.29478542 5.898642 0.0548250 0.00000000 0.11663333 0.11663333 0.289656792 0.001779863 TRUE TRUE
MSTRG.833.5 ENSG00000134698 HEK293_OSMI2_2hA HEK293_TMG_2hB AGO4 protein_coding   3.467385 1.793003 4.596424 0.04923307 0.4818984 1.353245 0.5663606 0.6392726 0.06535999 0.11505613 0.06535999 -3.106954 0.2243208 0.35966667 0.01396667 -0.34570000 0.001779863 0.001779863 TRUE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000134698 E001 0.1515154 0.0425306378 1.000000e+00   1 35807655 35807723 69 + 0.079 0.000 -8.179
ENSG00000134698 E002 0.4502799 0.0291950873 3.849016e-01 5.290463e-01 1 35808016 35808173 158 + 0.204 0.000 -12.030
ENSG00000134698 E003 1.6242331 0.0083131325 1.675900e-02 4.440479e-02 1 35808174 35808317 144 + 0.505 0.000 -13.820
ENSG00000134698 E004 2.0659802 0.0092293174 6.022621e-03 1.867188e-02 1 35808318 35808435 118 + 0.580 0.000 -14.092
ENSG00000134698 E005 12.4591242 0.0192862511 1.243149e-01 2.261505e-01 1 35816882 35817047 166 + 1.153 0.952 -0.733
ENSG00000134698 E006 18.7592624 0.0192627708 4.862461e-03 1.554833e-02 1 35822862 35822982 121 + 1.343 1.002 -1.216
ENSG00000134698 E007 25.2193525 0.0104784830 7.296259e-03 2.199151e-02 1 35825313 35825494 182 + 1.451 1.186 -0.923
ENSG00000134698 E008 20.3138095 0.0009141920 4.881033e-02 1.070449e-01 1 35825679 35825815 137 + 1.346 1.169 -0.623
ENSG00000134698 E009 20.8068410 0.0008776407 4.355394e-03 1.413642e-02 1 35825926 35826060 135 + 1.377 1.120 -0.905
ENSG00000134698 E010 19.6209840 0.0019830102 3.580523e-03 1.195593e-02 1 35826748 35826835 88 + 1.358 1.083 -0.972
ENSG00000134698 E011 23.7192671 0.0008130101 4.732518e-05 2.682067e-04 1 35831427 35831574 148 + 1.444 1.084 -1.269
ENSG00000134698 E012 21.1523238 0.0009662527 5.124492e-02 1.113670e-01 1 35831812 35831931 120 + 1.358 1.185 -0.608
ENSG00000134698 E013 20.5165391 0.0090078992 1.000330e-01 1.905327e-01 1 35832057 35832185 129 + 1.350 1.184 -0.583
ENSG00000134698 E014 19.6583406 0.0059758234 3.422311e-01 4.862294e-01 1 35832437 35832570 134 + 1.318 1.226 -0.322
ENSG00000134698 E015 23.0221890 0.0008301816 4.158635e-01 5.592085e-01 1 35833990 35834174 185 + 1.373 1.302 -0.246
ENSG00000134698 E016 22.8283734 0.0008961547 8.246288e-01 8.895313e-01 1 35835834 35835993 160 + 1.346 1.356 0.034
ENSG00000134698 E017 0.5890081 0.0210054685 3.400060e-01 4.839448e-01 1 35840095 35840134 40 + 0.146 0.313 1.401
ENSG00000134698 E018 30.9200929 0.0006519146 5.872015e-01 7.105400e-01 1 35841165 35841480 316 + 1.465 1.494 0.098
ENSG00000134698 E019 15.5032409 0.0011482617 2.739725e-03 9.491664e-03 1 35841616 35841672 57 + 1.093 1.346 0.893
ENSG00000134698 E020 18.1604047 0.0010392107 1.365636e-02 3.744418e-02 1 35841673 35841750 78 + 1.182 1.376 0.681
ENSG00000134698 E021 18.8064264 0.0010332491 4.398881e-01 5.816227e-01 1 35850157 35850258 102 + 1.246 1.302 0.200
ENSG00000134698 E022 25.5070048 0.0008421942 6.168752e-01 7.348254e-01 1 35850854 35851053 200 + 1.384 1.413 0.101
ENSG00000134698 E023 1.5824265 0.1549996595 8.806440e-01 9.274480e-01 1 35851054 35851195 142 + 0.380 0.414 0.189
ENSG00000134698 E024 1.7359034 0.0080344431 1.991344e-01 3.251378e-01 1 35853488 35853496 9 + 0.342 0.562 1.140
ENSG00000134698 E025 23.6316837 0.0008833286 3.845755e-01 5.287171e-01 1 35853497 35853702 206 + 1.387 1.313 -0.257
ENSG00000134698 E026 49.7992062 0.0005312618 1.769196e-01 2.972356e-01 1 35853703 35854676 974 + 1.701 1.621 -0.272
ENSG00000134698 E027 21.0498857 0.0008997963 9.751471e-01 9.885206e-01 1 35854677 35854822 146 + 1.318 1.314 -0.016
ENSG00000134698 E028 54.8799905 0.0004507082 7.782794e-02 1.559910e-01 1 35854823 35855701 879 + 1.690 1.769 0.266
ENSG00000134698 E029 154.9162548 0.0002660473 1.002995e-13 2.850072e-12 1 35855702 35857890 2189 + 2.095 2.282 0.627