ENSG00000134644

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000257075 ENSG00000134644 HEK293_OSMI2_2hA HEK293_TMG_2hB PUM1 protein_coding protein_coding 38.2536 21.83558 54.79523 1.322963 0.749533 1.326972 13.4774054 6.641698 19.189796 1.104947 3.9140479 1.5292963 0.33631667 0.3117667 0.35126667 0.03950000 8.920771e-01 9.334836e-13 FALSE TRUE
ENST00000373747 ENSG00000134644 HEK293_OSMI2_2hA HEK293_TMG_2hB PUM1 protein_coding protein_coding 38.2536 21.83558 54.79523 1.322963 0.749533 1.326972 6.3100873 7.051696 8.665920 1.047694 3.3794763 0.2970021 0.22393333 0.3202333 0.15756667 -0.16266667 3.051177e-01 9.334836e-13 FALSE TRUE
ENST00000424085 ENSG00000134644 HEK293_OSMI2_2hA HEK293_TMG_2hB PUM1 protein_coding protein_coding 38.2536 21.83558 54.79523 1.322963 0.749533 1.326972 2.1536225 4.850421 1.015365 2.179482 0.1980487 -2.2449436 0.09377917 0.2114667 0.01863333 -0.19283333 1.002836e-02 9.334836e-13 FALSE TRUE
ENST00000440538 ENSG00000134644 HEK293_OSMI2_2hA HEK293_TMG_2hB PUM1 protein_coding protein_coding 38.2536 21.83558 54.79523 1.322963 0.749533 1.326972 6.1005495 0.000000 9.798498 0.000000 2.9498768 9.9378884 0.11142083 0.0000000 0.17940000 0.17940000 9.334836e-13 9.334836e-13 FALSE TRUE
MSTRG.729.19 ENSG00000134644 HEK293_OSMI2_2hA HEK293_TMG_2hB PUM1 protein_coding   38.2536 21.83558 54.79523 1.322963 0.749533 1.326972 0.8636509 0.000000 4.902299 0.000000 3.0344075 8.9402545 0.01589583 0.0000000 0.08843333 0.08843333 2.693233e-01 9.334836e-13 FALSE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000134644 E001 530.5627717 0.0111360851 1.772544e-14 5.628615e-13 1 30931506 30931813 308 - 2.478 2.953 1.580
ENSG00000134644 E002 514.4266580 0.0035623739 2.433771e-51 1.606518e-48 1 30931814 30932058 245 - 2.415 2.970 1.847
ENSG00000134644 E003 740.0641492 0.0011549874 6.531676e-55 5.403522e-52 1 30932059 30932834 776 - 2.681 3.057 1.251
ENSG00000134644 E004 99.2418560 0.0082195670 6.049325e-07 5.265698e-06 1 30932835 30932843 9 - 1.830 2.172 1.148
ENSG00000134644 E005 85.3712220 0.0051489734 6.041989e-08 6.402487e-07 1 30932844 30932849 6 - 1.768 2.107 1.139
ENSG00000134644 E006 207.8415045 0.0138546512 3.098429e-04 1.419594e-03 1 30932850 30932954 105 - 2.179 2.461 0.942
ENSG00000134644 E007 243.4438572 0.0050669957 3.503766e-04 1.581516e-03 1 30932955 30933079 125 - 2.283 2.493 0.701
ENSG00000134644 E008 423.8146103 0.0047007285 3.414094e-01 4.853010e-01 1 30933080 30933304 225 - 2.570 2.672 0.340
ENSG00000134644 E009 241.5120570 0.0029846223 8.336527e-01 8.958196e-01 1 30933305 30933342 38 - 2.340 2.412 0.243
ENSG00000134644 E010 279.6534171 0.0028197056 6.696761e-01 7.763834e-01 1 30936643 30936721 79 - 2.400 2.479 0.263
ENSG00000134644 E011 138.5920955 0.0024635614 7.130365e-01 8.095907e-01 1 30936722 30936723 2 - 2.109 2.156 0.158
ENSG00000134644 E012 279.6364101 0.0012447169 7.889643e-01 8.648947e-01 1 30936724 30936835 112 - 2.403 2.473 0.233
ENSG00000134644 E013 312.2766661 0.0002673019 2.604224e-01 3.979500e-01 1 30941151 30941272 122 - 2.462 2.500 0.125
ENSG00000134644 E014 2.9634420 0.0056096954 2.430408e-01 3.777636e-01 1 30941922 30941997 76 - 0.625 0.474 -0.703
ENSG00000134644 E015 314.6260420 0.0033560821 1.075706e-01 2.019535e-01 1 30941998 30942123 126 - 2.476 2.477 0.004
ENSG00000134644 E016 245.5067449 0.0003040611 8.819691e-04 3.551025e-03 1 30945346 30945400 55 - 2.377 2.355 -0.071
ENSG00000134644 E017 284.3093587 0.0002254308 1.393471e-05 8.954587e-05 1 30945401 30945483 83 - 2.446 2.408 -0.128
ENSG00000134644 E018 63.0659084 0.0071763284 1.674112e-01 2.848357e-01 1 30950127 30950132 6 - 1.793 1.763 -0.101
ENSG00000134644 E019 318.7116888 0.0001471833 1.381057e-05 8.880795e-05 1 30950133 30950261 129 - 2.495 2.464 -0.103
ENSG00000134644 E020 268.8315123 0.0001703667 3.544962e-06 2.611230e-05 1 30952234 30952363 130 - 2.425 2.378 -0.156
ENSG00000134644 E021 215.9426122 0.0002012356 3.421679e-05 2.006087e-04 1 30953714 30953818 105 - 2.331 2.285 -0.155
ENSG00000134644 E022 245.1063841 0.0015771094 1.232301e-05 8.017301e-05 1 30953819 30953981 163 - 2.394 2.317 -0.255
ENSG00000134644 E023 298.7994954 0.0035930028 4.234186e-04 1.864173e-03 1 30964674 30964887 214 - 2.478 2.406 -0.240
ENSG00000134644 E024 121.8501292 0.0068564492 4.029001e-03 1.322335e-02 1 30964888 30964910 23 - 2.098 1.999 -0.331
ENSG00000134644 E025 128.0431703 0.0157688468 2.262123e-02 5.696410e-02 1 30965982 30966015 34 - 2.122 2.015 -0.359
ENSG00000134644 E026 203.0336188 0.0033459930 4.061301e-03 1.331398e-02 1 30966016 30966146 131 - 2.305 2.251 -0.180
ENSG00000134644 E027 138.9071443 0.0017502535 1.467128e-03 5.530324e-03 1 30966147 30966197 51 - 2.143 2.082 -0.206
ENSG00000134644 E028 89.2638164 0.0072689144 1.174719e-01 2.163380e-01 1 30966198 30966278 81 - 1.943 1.909 -0.115
ENSG00000134644 E029 2.9507314 0.0058033155 2.187690e-02 5.542201e-02 1 30966279 30966380 102 - 0.667 0.299 -1.882
ENSG00000134644 E030 4.3649771 0.0038174295 2.421357e-01 3.767199e-01 1 30967099 30967166 68 - 0.625 0.840 0.882
ENSG00000134644 E031 120.8377487 0.0002830077 5.569603e-07 4.884250e-06 1 30967167 30967224 58 - 2.100 1.984 -0.389
ENSG00000134644 E032 137.3139461 0.0002960265 9.174200e-05 4.831604e-04 1 30967225 30967310 86 - 2.141 2.074 -0.226
ENSG00000134644 E033 2.6091282 0.0537247035 7.124373e-01 8.091770e-01 1 30968297 30968353 57 - 0.497 0.600 0.477
ENSG00000134644 E034 157.0382350 0.0002819859 1.425242e-04 7.144785e-04 1 30968354 30968492 139 - 2.197 2.142 -0.184
ENSG00000134644 E035 1.7618140 0.2654578282 6.914725e-01 7.929384e-01 1 30969211 30969276 66 - 0.397 0.476 0.411
ENSG00000134644 E036 0.1451727 0.0430203498 1.000000e+00   1 30974649 30974650 2 - 0.084 0.000 -8.571
ENSG00000134644 E037 161.9706792 0.0002177076 2.000016e-05 1.240699e-04 1 30974651 30974802 152 - 2.214 2.147 -0.223
ENSG00000134644 E038 0.5117906 0.1833794180 9.804507e-01 9.917660e-01 1 30974803 30974859 57 - 0.155 0.176 0.225
ENSG00000134644 E039 125.1826811 0.0004693629 4.756316e-03 1.525066e-02 1 30980062 30980163 102 - 2.091 2.054 -0.124
ENSG00000134644 E040 32.4639373 0.0006434061 8.323216e-02 1.646281e-01 1 30980164 30980166 3 - 1.523 1.472 -0.177
ENSG00000134644 E041 2.4431175 0.0062742971 8.514049e-01 9.078614e-01 1 30980167 30980178 12 - 0.526 0.540 0.065
ENSG00000134644 E042 80.6122398 0.0006667076 1.904320e-01 3.143014e-01 1 30981312 30981337 26 - 1.890 1.895 0.018
ENSG00000134644 E043 115.7423243 0.0002523715 3.504141e-04 1.581647e-03 1 30981338 30981405 68 - 2.067 2.001 -0.219
ENSG00000134644 E044 0.5954526 0.0184274670 1.499136e-01 2.615693e-01 1 30981917 30982145 229 - 0.269 0.000 -10.572
ENSG00000134644 E045 142.5265592 0.0012355356 6.703534e-07 5.778870e-06 1 30992390 30992440 51 - 2.172 2.050 -0.407
ENSG00000134644 E046 277.8745703 0.0040167150 2.050432e-06 1.591153e-05 1 30992441 30992660 220 - 2.463 2.336 -0.423
ENSG00000134644 E047 285.4626886 0.0019304931 1.070516e-12 2.612135e-11 1 30995054 30995181 128 - 2.486 2.320 -0.552
ENSG00000134644 E048 202.0252921 0.0002003642 1.522577e-16 6.408522e-15 1 30995182 30995220 39 - 2.337 2.168 -0.565
ENSG00000134644 E049 304.3956904 0.0011852375 2.474162e-20 1.642335e-18 1 31005853 31006031 179 - 2.522 2.321 -0.670
ENSG00000134644 E050 220.5648057 0.0002011918 5.046923e-20 3.214945e-18 1 31006994 31007099 106 - 2.379 2.193 -0.620
ENSG00000134644 E051 90.9463667 0.0003597969 3.816628e-06 2.790078e-05 1 31007100 31007102 3 - 1.980 1.852 -0.431
ENSG00000134644 E052 0.2965864 0.0884183793 8.208587e-02   1 31028758 31028795 38 - 0.000 0.296 11.689
ENSG00000134644 E053 182.4352871 0.0002359339 3.096459e-11 5.951855e-10 1 31028796 31028864 69 - 2.282 2.150 -0.442
ENSG00000134644 E054 1.5135278 0.0596906543 8.329646e-01 8.953647e-01 1 31055313 31055380 68 - 0.398 0.393 -0.027
ENSG00000134644 E055 178.8685064 0.0002057018 7.428313e-12 1.578898e-10 1 31059204 31059278 75 - 2.278 2.139 -0.464
ENSG00000134644 E056 120.1711652 0.0002484156 3.725219e-09 4.965744e-08 1 31059279 31059298 20 - 2.109 1.961 -0.498
ENSG00000134644 E057 301.7222364 0.0001535232 1.439617e-22 1.223009e-20 1 31059299 31059577 279 - 2.510 2.350 -0.536
ENSG00000134644 E058 0.3729606 0.0261799519 6.102834e-01 7.296203e-01 1 31061667 31061916 250 - 0.084 0.175 1.206
ENSG00000134644 E059 109.4100675 0.0002706368 1.985100e-06 1.545329e-05 1 31065616 31065688 73 - 2.060 1.946 -0.382
ENSG00000134644 E060 31.7706098 0.0035418749 1.357187e-02 3.724254e-02 1 31065689 31065991 303 - 1.534 1.417 -0.402