ENSG00000134597

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000305536 ENSG00000134597 HEK293_OSMI2_2hA HEK293_TMG_2hB RBMX2 protein_coding protein_coding 28.85591 31.21327 29.61708 5.129809 0.6001732 -0.07570498 24.320935 28.01041 23.419033 4.3907632 0.8421751 -0.2581807 0.8442167 0.89950000 0.7912333 -0.10826667 0.01085172 0.002864736 FALSE TRUE
ENST00000469953 ENSG00000134597 HEK293_OSMI2_2hA HEK293_TMG_2hB RBMX2 protein_coding processed_transcript 28.85591 31.21327 29.61708 5.129809 0.6001732 -0.07570498 3.318974 2.76147 4.498056 0.7092096 0.7192428 0.7018542 0.1144208 0.08583333 0.1514667 0.06563333 0.09487640 0.002864736   FALSE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000134597 E001 47.502571 0.0005954148 3.423664e-02 8.021147e-02 X 130401987 130402000 14 + 1.738 1.639 -0.337
ENSG00000134597 E002 61.361134 0.0021130142 1.953958e-01 3.204504e-01 X 130402001 130402004 4 + 1.825 1.765 -0.202
ENSG00000134597 E003 115.596024 0.0029177659 4.687374e-01 6.081212e-01 X 130402005 130402037 33 + 2.080 2.049 -0.101
ENSG00000134597 E004 18.962385 0.0065730636 2.340767e-01 3.672443e-01 X 130402038 130402254 217 + 1.354 1.262 -0.322
ENSG00000134597 E005 240.427760 0.0002157752 1.181706e-01 2.173142e-01 X 130402255 130402370 116 + 2.397 2.370 -0.090
ENSG00000134597 E006 223.827310 0.0002345538 5.988735e-02 1.264205e-01 X 130403802 130403853 52 + 2.371 2.336 -0.118
ENSG00000134597 E007 10.083310 0.0705009581 3.894245e-06 2.839806e-05 X 130403854 130404450 597 + 1.341 0.558 -3.001
ENSG00000134597 E008 299.090777 0.0002395161 5.144968e-03 1.632388e-02 X 130409257 130409386 130 + 2.505 2.458 -0.156
ENSG00000134597 E009 4.915275 0.0042307519 3.793295e-01 5.235908e-01 X 130411203 130411347 145 + 0.836 0.727 -0.432
ENSG00000134597 E010 227.178731 0.0017735005 3.109560e-01 4.533990e-01 X 130411348 130411525 178 + 2.370 2.348 -0.075
ENSG00000134597 E011 840.673172 0.0005014283 3.210939e-11 6.153796e-10 X 130412361 130413656 1296 + 2.871 2.954 0.276