ENSG00000134574

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000256996 ENSG00000134574 HEK293_OSMI2_2hA HEK293_TMG_2hB DDB2 protein_coding protein_coding 14.28139 23.98829 7.423738 1.389499 0.1590936 -1.690772 5.5332292 9.8483477 4.0640633 0.63523936 0.1399550 -1.2748775 0.40128333 0.41280000 0.54740000 0.1346000000 0.04996507 0.03913427 FALSE TRUE
ENST00000378600 ENSG00000134574 HEK293_OSMI2_2hA HEK293_TMG_2hB DDB2 protein_coding protein_coding 14.28139 23.98829 7.423738 1.389499 0.1590936 -1.690772 1.5021077 4.0937874 0.3585887 0.35122381 0.1949007 -3.4768724 0.08095417 0.17023333 0.04780000 -0.1224333333 0.21588458 0.03913427 FALSE TRUE
ENST00000378601 ENSG00000134574 HEK293_OSMI2_2hA HEK293_TMG_2hB DDB2 protein_coding nonsense_mediated_decay 14.28139 23.98829 7.423738 1.389499 0.1590936 -1.690772 1.5421695 1.5659685 0.6294179 0.07911584 0.3195705 -1.3014077 0.13145833 0.06596667 0.08640000 0.0204333333 0.96270882 0.03913427 TRUE TRUE
ENST00000614884 ENSG00000134574 HEK293_OSMI2_2hA HEK293_TMG_2hB DDB2 protein_coding retained_intron 14.28139 23.98829 7.423738 1.389499 0.1590936 -1.690772 2.1036380 4.5753906 0.2020605 0.72034582 0.2020605 -4.4344969 0.10177500 0.18926667 0.02616667 -0.1631000000 0.06112193 0.03913427   FALSE
ENST00000615695 ENSG00000134574 HEK293_OSMI2_2hA HEK293_TMG_2hB DDB2 protein_coding retained_intron 14.28139 23.98829 7.423738 1.389499 0.1590936 -1.690772 0.9954943 1.9494063 0.6090252 0.26684850 0.1077158 -1.6623466 0.06987500 0.08096667 0.08150000 0.0005333333 1.00000000 0.03913427 FALSE TRUE
ENST00000617022 ENSG00000134574 HEK293_OSMI2_2hA HEK293_TMG_2hB DDB2 protein_coding retained_intron 14.28139 23.98829 7.423738 1.389499 0.1590936 -1.690772 0.8960283 0.3583387 0.5349720 0.18448335 0.0501672 0.5651489 0.08200833 0.01580000 0.07213333 0.0563333333 0.20305028 0.03913427 FALSE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000134574 E001 1.252300 0.1789368263 4.337364e-01 5.761388e-01 11 47214354 47214450 97 + 0.421 0.254 -1.040
ENSG00000134574 E002 1.615708 0.0235949052 6.552023e-01 7.651432e-01 11 47214451 47214464 14 + 0.421 0.336 -0.485
ENSG00000134574 E003 1.615708 0.0235949052 6.552023e-01 7.651432e-01 11 47214465 47214467 3 + 0.421 0.336 -0.485
ENSG00000134574 E004 2.124290 0.0487622339 6.332713e-01 7.480069e-01 11 47214468 47214506 39 + 0.503 0.409 -0.477
ENSG00000134574 E005 2.715374 0.0106895559 3.364451e-01 4.802951e-01 11 47214507 47214521 15 + 0.631 0.469 -0.753
ENSG00000134574 E006 3.196785 0.0061208272 9.885580e-01 9.969033e-01 11 47214522 47214569 48 + 0.572 0.570 -0.007
ENSG00000134574 E007 3.274104 0.0127402842 9.197110e-01 9.532788e-01 11 47214570 47214639 70 + 0.571 0.591 0.089
ENSG00000134574 E008 2.757116 0.0217584007 8.291669e-01 8.927058e-01 11 47214640 47214674 35 + 0.503 0.545 0.201
ENSG00000134574 E009 4.362812 0.0036528890 1.575790e-02 4.219010e-02 11 47214974 47214991 18 + 0.908 0.570 -1.386
ENSG00000134574 E010 31.439708 0.0010456085 4.577479e-01 5.980613e-01 11 47214992 47215044 53 + 1.476 1.425 -0.175
ENSG00000134574 E011 35.529881 0.0010269958 6.999292e-02 1.433601e-01 11 47215045 47215057 13 + 1.575 1.459 -0.398
ENSG00000134574 E012 37.345277 0.0007401709 8.974842e-02 1.747438e-01 11 47215058 47215070 13 + 1.587 1.482 -0.361
ENSG00000134574 E013 33.141255 0.0008823186 2.708485e-01 4.095063e-01 11 47215071 47215072 2 + 1.514 1.441 -0.251
ENSG00000134574 E014 44.094139 0.0005095469 4.878089e-01 6.254476e-01 11 47215073 47215087 15 + 1.611 1.572 -0.135
ENSG00000134574 E015 43.537586 0.0005302787 3.271689e-01 4.706132e-01 11 47215088 47215094 7 + 1.617 1.560 -0.193
ENSG00000134574 E016 50.389817 0.0004973402 6.663666e-01 7.738166e-01 11 47215095 47215111 17 + 1.655 1.633 -0.077
ENSG00000134574 E017 64.833834 0.0005626931 7.900413e-01 8.657004e-01 11 47215112 47215136 25 + 1.753 1.741 -0.040
ENSG00000134574 E018 103.812608 0.0011337468 4.804583e-01 6.187512e-01 11 47215137 47215263 127 + 1.924 1.955 0.103
ENSG00000134574 E019 2.649810 0.0086309022 5.504335e-01 6.800708e-01 11 47215264 47215272 9 + 0.572 0.469 -0.489
ENSG00000134574 E020 21.023054 0.0011476082 2.422756e-06 1.850741e-05 11 47215273 47216193 921 + 1.514 1.152 -1.262
ENSG00000134574 E021 7.875008 0.0102376552 7.697455e-02 1.546503e-01 11 47216194 47216335 142 + 1.034 0.810 -0.849
ENSG00000134574 E022 94.060390 0.0003060561 6.383961e-03 1.962074e-02 11 47216336 47216472 137 + 1.812 1.931 0.402
ENSG00000134574 E023 60.149284 0.0005358159 2.564836e-02 6.321475e-02 11 47216858 47216912 55 + 1.617 1.740 0.417
ENSG00000134574 E024 106.283614 0.0011003664 1.792813e-01 3.002211e-01 11 47216913 47217049 137 + 1.913 1.970 0.192
ENSG00000134574 E025 13.619905 0.0013940326 3.611580e-02 8.380938e-02 11 47217050 47217411 362 + 0.908 1.146 0.873
ENSG00000134574 E026 77.602219 0.0003801011 2.283501e-01 3.604599e-01 11 47232814 47232959 146 + 1.864 1.812 -0.175
ENSG00000134574 E027 1.368140 0.0128844106 2.415351e-01 3.759878e-01 11 47233227 47233474 248 + 0.503 0.294 -1.174
ENSG00000134574 E028 38.796703 0.0006704965 3.811363e-02 8.756807e-02 11 47234573 47234580 8 + 1.623 1.498 -0.427
ENSG00000134574 E029 51.296473 0.0004554948 3.356322e-02 7.889754e-02 11 47234581 47234626 46 + 1.732 1.620 -0.378
ENSG00000134574 E030 50.983650 0.0004612035 2.077776e-01 3.358044e-01 11 47234627 47234672 46 + 1.695 1.628 -0.227
ENSG00000134574 E031 1.948930 0.0117103994 8.459945e-02 1.667563e-01 11 47234673 47234756 84 + 0.631 0.335 -1.492
ENSG00000134574 E032 79.135077 0.0004185063 8.038381e-01 8.751895e-01 11 47234757 47234934 178 + 1.823 1.836 0.043
ENSG00000134574 E033 6.593554 0.0024675182 7.488111e-04 3.074423e-03 11 47234935 47235269 335 + 1.096 0.688 -1.563
ENSG00000134574 E034 85.858168 0.0048035953 9.932434e-01 9.999255e-01 11 47235270 47235412 143 + 1.864 1.866 0.010
ENSG00000134574 E035 7.396640 0.0026044528 3.609057e-01 5.052840e-01 11 47235413 47236121 709 + 0.937 0.822 -0.440
ENSG00000134574 E036 17.822710 0.0013082228 1.738402e-05 1.093301e-04 11 47236122 47237618 1497 + 1.452 1.102 -1.230
ENSG00000134574 E037 4.026398 0.0439337192 2.059392e-02 5.273592e-02 11 47237619 47237712 94 + 0.908 0.542 -1.515
ENSG00000134574 E038 4.915098 0.0245976488 1.484419e-01 2.595561e-01 11 47237713 47237805 93 + 0.878 0.667 -0.845
ENSG00000134574 E039 4.540047 0.0084887514 4.846881e-01 6.226493e-01 11 47237806 47237836 31 + 0.772 0.670 -0.418
ENSG00000134574 E040 157.808066 0.0007063364 2.057595e-02 5.270343e-02 11 47237837 47238001 165 + 2.065 2.145 0.268
ENSG00000134574 E041 106.323331 0.0011286578 9.991389e-03 2.874252e-02 11 47238138 47238183 46 + 1.870 1.982 0.375
ENSG00000134574 E042 1.631529 0.0115915870 3.466626e-01 4.907908e-01 11 47238184 47238799 616 + 0.503 0.335 -0.908
ENSG00000134574 E043 205.116066 0.0005708510 9.736994e-05 5.097392e-04 11 47238800 47239217 418 + 2.148 2.266 0.395