ENSG00000134318

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000315872 ENSG00000134318 HEK293_OSMI2_2hA HEK293_TMG_2hB ROCK2 protein_coding protein_coding 8.317423 1.821001 14.19441 0.2806527 0.5801886 2.955635 4.554835 1.739598 6.987171 0.2507304 0.3160311 1.999748 0.7188667 0.9589667 0.4920667 -0.4669000 2.200330e-22 5.735497e-25 FALSE TRUE
MSTRG.17960.2 ENSG00000134318 HEK293_OSMI2_2hA HEK293_TMG_2hB ROCK2 protein_coding   8.317423 1.821001 14.19441 0.2806527 0.5801886 2.955635 3.307292 0.000000 6.190022 0.0000000 0.2349068 9.276129 0.2327167 0.0000000 0.4362333 0.4362333 5.735497e-25 5.735497e-25 FALSE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000134318 E001 401.0020965 0.0274738925 1.363369e-09 1.970898e-08 2 11179759 11182974 3216 - 2.322 2.825 1.677
ENSG00000134318 E002 36.0638679 0.0011419021 5.118479e-03 1.625181e-02 2 11182975 11183121 147 - 1.343 1.644 1.034
ENSG00000134318 E003 68.2221043 0.0037390326 1.702992e-01 2.885620e-01 2 11183122 11183330 209 - 1.635 1.819 0.624
ENSG00000134318 E004 48.3925864 0.0031696659 4.723865e-02 1.042547e-01 2 11183331 11183440 110 - 1.477 1.714 0.811
ENSG00000134318 E005 4.1989165 0.0037570173 2.073461e-02 5.302599e-02 2 11184666 11184769 104 - 0.482 0.929 1.880
ENSG00000134318 E006 67.4970084 0.0004197462 2.051014e-02 5.256400e-02 2 11192148 11192361 214 - 1.620 1.847 0.767
ENSG00000134318 E007 68.9134480 0.0003579710 4.090747e-01 5.526885e-01 2 11192451 11192675 225 - 1.648 1.791 0.487
ENSG00000134318 E008 39.4078163 0.0005413229 7.037048e-01 8.023760e-01 2 11192676 11192712 37 - 1.417 1.540 0.424
ENSG00000134318 E009 52.5099150 0.0004176102 9.729381e-01 9.870992e-01 2 11193779 11193857 79 - 1.542 1.635 0.317
ENSG00000134318 E010 0.1472490 0.0447342501 8.356553e-01   2 11193910 11194255 346 - 0.047 0.000 -8.489
ENSG00000134318 E011 50.4554503 0.0004702589 8.207021e-01 8.869307e-01 2 11194256 11194344 89 - 1.526 1.606 0.271
ENSG00000134318 E012 49.5193221 0.0004804973 1.789223e-01 2.997920e-01 2 11194955 11195025 71 - 1.532 1.528 -0.012
ENSG00000134318 E013 0.0000000       2 11195026 11195210 185 -      
ENSG00000134318 E014 70.8728346 0.0004020740 9.989225e-01 1.000000e+00 2 11197180 11197348 169 - 1.668 1.764 0.325
ENSG00000134318 E015 82.8976164 0.0003446558 7.027492e-01 8.015276e-01 2 11197526 11197705 180 - 1.730 1.847 0.395
ENSG00000134318 E016 63.5302270 0.0004264865 4.747881e-01 6.135970e-01 2 11198491 11198585 95 - 1.611 1.750 0.473
ENSG00000134318 E017 64.0362318 0.0003666471 2.870832e-01 4.275511e-01 2 11198681 11198774 94 - 1.634 1.662 0.095
ENSG00000134318 E018 80.8538501 0.0003409473 3.606804e-02 8.371673e-02 2 11200957 11201143 187 - 1.739 1.712 -0.091
ENSG00000134318 E019 62.3836103 0.0020546770 6.371495e-02 1.328864e-01 2 11201310 11201413 104 - 1.629 1.596 -0.110
ENSG00000134318 E020 51.3594167 0.0180884000 4.425164e-02 9.884996e-02 2 11202052 11202121 70 - 1.555 1.455 -0.342
ENSG00000134318 E021 76.0545807 0.0003456280 1.614580e-05 1.023070e-04 2 11207726 11207910 185 - 1.731 1.540 -0.648
ENSG00000134318 E022 62.7765298 0.0004048108 3.623757e-04 1.628267e-03 2 11208287 11208447 161 - 1.650 1.491 -0.544
ENSG00000134318 E023 64.2141467 0.0003653345 6.498013e-03 1.991828e-02 2 11211681 11211840 160 - 1.652 1.563 -0.303
ENSG00000134318 E024 54.6342746 0.0004515897 4.957325e-04 2.143689e-03 2 11214357 11214463 107 - 1.592 1.420 -0.593
ENSG00000134318 E025 61.4111564 0.0004177976 4.095020e-05 2.354872e-04 2 11214840 11215086 247 - 1.644 1.435 -0.718
ENSG00000134318 E026 25.1476054 0.0027298495 5.681600e-02 1.211424e-01 2 11215321 11215359 39 - 1.265 1.149 -0.411
ENSG00000134318 E027 28.0515463 0.0042034601 2.582427e-01 3.954612e-01 2 11215360 11215412 53 - 1.296 1.274 -0.076
ENSG00000134318 E028 49.5606418 0.0072772481 2.874203e-03 9.895322e-03 2 11215510 11215645 136 - 1.551 1.371 -0.620
ENSG00000134318 E029 36.6501557 0.0057362856 3.971678e-03 1.306221e-02 2 11216158 11216206 49 - 1.426 1.229 -0.689
ENSG00000134318 E030 43.8585542 0.0005175579 1.046313e-04 5.434135e-04 2 11217090 11217169 80 - 1.505 1.252 -0.879
ENSG00000134318 E031 0.3729606 0.0283280369 1.726276e-01 2.916256e-01 2 11217776 11218314 539 - 0.047 0.287 3.051
ENSG00000134318 E032 0.4460135 0.0416068894 1.000000e+00 1.000000e+00 2 11218315 11218454 140 - 0.127 0.000 -10.074
ENSG00000134318 E033 26.0188957 0.0007626621 2.025951e-02 5.202867e-02 2 11218455 11218466 12 - 1.278 1.120 -0.563
ENSG00000134318 E034 30.2046809 0.0026628690 6.744163e-03 2.055903e-02 2 11218966 11219026 61 - 1.343 1.150 -0.682
ENSG00000134318 E035 51.2755300 0.0004258039 1.358820e-02 3.727818e-02 2 11221198 11221326 129 - 1.556 1.464 -0.316
ENSG00000134318 E036 34.8632653 0.0020230554 5.668792e-04 2.409753e-03 2 11221327 11221357 31 - 1.407 1.150 -0.904
ENSG00000134318 E037 48.1093717 0.0021927314 4.953509e-02 1.083422e-01 2 11222083 11222174 92 - 1.525 1.464 -0.207
ENSG00000134318 E038 54.2683348 0.0004663261 2.457104e-03 8.635076e-03 2 11224322 11224460 139 - 1.584 1.450 -0.459
ENSG00000134318 E039 68.2750023 0.0504230309 5.939823e-03 1.845057e-02 2 11227254 11227398 145 - 1.691 1.456 -0.801
ENSG00000134318 E040 0.1482932 0.0468791072 5.311773e-02   2 11234204 11234670 467 - 0.000 0.286 14.300
ENSG00000134318 E041 69.8828517 0.0006260370 2.455028e-07 2.312883e-06 2 11235702 11235883 182 - 1.704 1.420 -0.971
ENSG00000134318 E042 34.0532407 0.0006621069 3.413769e-05 2.002193e-04 2 11235884 11235962 79 - 1.404 1.053 -1.240
ENSG00000134318 E043 36.7493392 0.0545283389 2.563507e-02 6.319355e-02 2 11249661 11249798 138 - 1.424 1.219 -0.717
ENSG00000134318 E044 24.0722452 0.0008241345 3.787841e-01 5.230857e-01 2 11286539 11286639 101 - 1.226 1.228 0.008
ENSG00000134318 E045 19.8547280 0.0009585698 4.108113e-01 5.543982e-01 2 11287655 11287736 82 - 1.150 1.150 -0.002
ENSG00000134318 E046 0.1515154 0.0431917941 8.345122e-01   2 11287737 11287737 1 - 0.047 0.000 -8.490
ENSG00000134318 E047 0.6653823 0.0172671820 5.021560e-01 6.382394e-01 2 11288350 11288403 54 - 0.127 0.287 1.465
ENSG00000134318 E048 34.0298431 0.0031282901 1.802434e-02 4.718397e-02 2 11343996 11344636 641 - 1.388 1.252 -0.473
ENSG00000134318 E049 0.6631923 0.5373666110 4.941927e-01 6.311656e-01 2 11345274 11345316 43 - 0.127 0.294 1.515
ENSG00000134318 E050 0.0000000       2 11348168 11348330 163 -