ENSG00000134253

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000256649 ENSG00000134253 HEK293_OSMI2_2hA HEK293_TMG_2hB TRIM45 protein_coding protein_coding 3.570998 3.033441 2.380743 0.2037424 0.118233 -0.3482443 2.7711711 2.53352161 1.7852477 0.15376555 0.07333957 -0.5026445 0.77135000 0.8409 0.7515667 -0.08933333 0.52696883 0.01152199 FALSE TRUE
ENST00000485032 ENSG00000134253 HEK293_OSMI2_2hA HEK293_TMG_2hB TRIM45 protein_coding protein_coding 3.570998 3.033441 2.380743 0.2037424 0.118233 -0.3482443 0.3311159 0.05160133 0.3958275 0.05160133 0.06370544 2.7198334 0.09230833 0.0192 0.1645667 0.14536667 0.01152199 0.01152199 FALSE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000134253 E001 0.2924217 0.0290785164 0.100311891   1 117110615 117110696 82 - 0.263 0.000 -11.212
ENSG00000134253 E002 0.9243042 0.0144735831 0.552908424 0.68213211 1 117110697 117111022 326 - 0.352 0.246 -0.714
ENSG00000134253 E003 0.3299976 0.0274424043 0.367423706   1 117111023 117111059 37 - 0.000 0.178 11.600
ENSG00000134253 E004 0.1482932 0.0412343369 0.748201153   1 117111060 117111067 8 - 0.000 0.098 10.583
ENSG00000134253 E005 13.5422328 0.1217669115 0.708391079 0.80604562 1 117111068 117111212 145 - 1.103 1.165 0.224
ENSG00000134253 E006 60.3808963 0.0009181630 0.008348948 0.02466255 1 117111213 117112094 882 - 1.686 1.804 0.402
ENSG00000134253 E007 12.2910873 0.0319022947 0.450540824 0.59163639 1 117112095 117112184 90 - 1.156 1.055 -0.364
ENSG00000134253 E008 28.2769980 0.0007505698 0.964445314 0.98160621 1 117112185 117112453 269 - 1.442 1.443 0.003
ENSG00000134253 E009 24.5575822 0.0082965508 0.182665465 0.30453139 1 117113359 117113485 127 - 1.313 1.420 0.372
ENSG00000134253 E010 28.7810588 0.0034540586 0.311832784 0.45435572 1 117115575 117115689 115 - 1.408 1.476 0.236
ENSG00000134253 E011 28.7570690 0.0007741612 0.418627172 0.56186796 1 117116616 117116745 130 - 1.491 1.438 -0.182
ENSG00000134253 E012 15.2316459 0.0087023083 0.525833451 0.65915589 1 117118034 117118087 54 - 1.156 1.216 0.212
ENSG00000134253 E013 76.2478007 0.0007244667 0.803555074 0.87498280 1 117118088 117118767 680 - 1.878 1.867 -0.038
ENSG00000134253 E014 32.3722337 0.0126239221 0.305601190 0.44772776 1 117120714 117120901 188 - 1.551 1.468 -0.285
ENSG00000134253 E015 15.2247779 0.0168177779 0.450079438 0.59124772 1 117120902 117120964 63 - 1.236 1.155 -0.289
ENSG00000134253 E016 40.1129686 0.0061927633 0.102630720 0.19451522 1 117120965 117121600 636 - 1.655 1.547 -0.367
ENSG00000134253 E017 1.5865635 0.0093349502 0.769711416 0.85129205 1 117121601 117121605 5 - 0.352 0.403 0.290
ENSG00000134253 E018 5.0648279 0.0039119949 0.061207959 0.12866202 1 117121606 117121792 187 - 0.907 0.659 -0.992
ENSG00000134253 E019 1.2147619 0.1611815619 0.821466113 0.88742998 1 117121799 117122612 814 - 0.352 0.308 -0.277