ENSG00000134186

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000370025 ENSG00000134186 HEK293_OSMI2_2hA HEK293_TMG_2hB PRPF38B protein_coding protein_coding 46.44651 14.88341 80.61927 0.4143554 0.6786048 2.436629 20.337566 9.1907931 34.57952 0.2967443 1.0322696 1.910505 0.47777083 0.61953333 0.4290333 -0.19050000 0.001810738 0.001810738 FALSE TRUE
ENST00000467302 ENSG00000134186 HEK293_OSMI2_2hA HEK293_TMG_2hB PRPF38B protein_coding processed_transcript 46.44651 14.88341 80.61927 0.4143554 0.6786048 2.436629 5.764553 1.2214875 10.57835 0.1016977 0.3550291 3.104004 0.11643333 0.08206667 0.1312333 0.04916667 0.005159160 0.001810738 TRUE FALSE
ENST00000485810 ENSG00000134186 HEK293_OSMI2_2hA HEK293_TMG_2hB PRPF38B protein_coding processed_transcript 46.44651 14.88341 80.61927 0.4143554 0.6786048 2.436629 8.560532 1.8058950 14.13772 0.3297656 0.3584390 2.961817 0.17708750 0.12136667 0.1753333 0.05396667 0.204304461 0.001810738 FALSE FALSE
MSTRG.1723.14 ENSG00000134186 HEK293_OSMI2_2hA HEK293_TMG_2hB PRPF38B protein_coding   46.44651 14.88341 80.61927 0.4143554 0.6786048 2.436629 5.094298 0.4939742 11.70052 0.4939742 0.9348986 4.538311 0.07482917 0.03150000 0.1449667 0.11346667 0.107372864 0.001810738 TRUE FALSE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000134186 E001 0.1472490 0.0430289228 1.000000e+00   1 108691836 108692252 417 + 0.061 0.000 -8.924
ENSG00000134186 E002 0.1472490 0.0430289228 1.000000e+00   1 108692253 108692272 20 + 0.061 0.000 -8.909
ENSG00000134186 E003 0.4417471 0.6756312805 1.000000e+00 1.000000e+00 1 108692273 108692304 32 + 0.162 0.000 -10.492
ENSG00000134186 E004 3.9492595 0.0040369468 9.396297e-01 9.660863e-01 1 108692305 108692309 5 + 0.620 0.654 0.143
ENSG00000134186 E005 10.7207620 0.0082987642 6.992777e-01 7.990758e-01 1 108692310 108692323 14 + 0.962 1.062 0.368
ENSG00000134186 E006 11.7818078 0.0081714176 7.906282e-01 8.660877e-01 1 108692324 108692326 3 + 1.003 1.087 0.310
ENSG00000134186 E007 28.6688871 0.0344719754 4.819012e-01 6.200945e-01 1 108692327 108692334 8 + 1.390 1.369 -0.073
ENSG00000134186 E008 43.9978694 0.0173405598 9.466708e-01 9.705157e-01 1 108692335 108692340 6 + 1.553 1.605 0.178
ENSG00000134186 E009 69.2987973 0.0164948332 8.266001e-01 8.908992e-01 1 108692341 108692363 23 + 1.740 1.814 0.251
ENSG00000134186 E010 72.6297770 0.0142221706 8.996292e-01 9.399466e-01 1 108692364 108692369 6 + 1.762 1.827 0.221
ENSG00000134186 E011 109.6622619 0.0038134787 9.538835e-01 9.751224e-01 1 108692370 108692450 81 + 1.939 2.000 0.203
ENSG00000134186 E012 113.4756877 0.0006810948 2.188593e-01 3.490411e-01 1 108692451 108692524 74 + 1.967 1.974 0.023
ENSG00000134186 E013 369.0043385 0.0022672595 2.307103e-02 5.789593e-02 1 108692525 108692867 343 + 2.479 2.465 -0.046
ENSG00000134186 E014 8.8026040 0.0176362757 1.540290e-01 2.671507e-01 1 108693590 108693642 53 + 0.940 0.771 -0.651
ENSG00000134186 E015 253.4642139 0.0002341218 1.006589e-05 6.683790e-05 1 108695702 108695770 69 + 2.326 2.258 -0.227
ENSG00000134186 E016 18.5151049 0.0939749410 7.390908e-02 1.497605e-01 1 108695771 108695780 10 + 1.243 0.984 -0.934
ENSG00000134186 E017 276.6231336 0.0001540447 4.537054e-02 1.008733e-01 1 108696043 108696194 152 + 2.350 2.357 0.022
ENSG00000134186 E018 162.0401835 0.0002446703 7.346334e-01 8.255475e-01 1 108696277 108696337 61 + 2.106 2.177 0.236
ENSG00000134186 E019 24.5212819 0.0107413891 1.611358e-01 2.765890e-01 1 108696338 108696351 14 + 1.337 1.250 -0.303
ENSG00000134186 E020 88.8557383 0.0003653304 3.988659e-05 2.299713e-04 1 108696352 108696739 388 + 1.887 1.740 -0.496
ENSG00000134186 E021 172.5540402 0.0001952332 3.696666e-18 1.897892e-16 1 108696740 108697504 765 + 2.192 1.919 -0.914
ENSG00000134186 E022 32.0800530 0.0006588201 2.312375e-04 1.096799e-03 1 108697700 108697700 1 + 1.473 1.215 -0.896
ENSG00000134186 E023 60.6627046 0.0003967214 9.384088e-06 6.278260e-05 1 108697701 108697809 109 + 1.737 1.522 -0.734
ENSG00000134186 E024 53.0048110 0.0004041318 1.560753e-05 9.920148e-05 1 108697810 108697863 54 + 1.681 1.452 -0.781
ENSG00000134186 E025 65.6494113 0.0045586960 6.145311e-04 2.586561e-03 1 108697864 108697946 83 + 1.763 1.590 -0.588
ENSG00000134186 E026 79.6678349 0.0053613110 1.119606e-01 2.084313e-01 1 108697947 108698017 71 + 1.823 1.783 -0.136
ENSG00000134186 E027 109.9781017 0.0118099465 3.370892e-03 1.135278e-02 1 108698018 108698603 586 + 1.979 1.831 -0.498
ENSG00000134186 E028 445.4079166 0.0003013379 9.257009e-01 9.572354e-01 1 108698604 108698827 224 + 2.545 2.606 0.204
ENSG00000134186 E029 645.6661161 0.0008413352 2.693345e-01 4.078543e-01 1 108699162 108699466 305 + 2.712 2.748 0.122
ENSG00000134186 E030 438.4350020 0.0008819623 1.414595e-01 2.500203e-01 1 108699467 108699570 104 + 2.547 2.570 0.078
ENSG00000134186 E031 1802.6095628 0.0050028381 2.497405e-07 2.349419e-06 1 108699571 108702928 3358 + 3.119 3.305 0.618