ENSG00000134086

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000256474 ENSG00000134086 HEK293_OSMI2_2hA HEK293_TMG_2hB VHL protein_coding protein_coding 43.79489 35.36838 58.70634 5.744034 0.9190629 0.7308938 32.565465 20.549353 49.76216573 0.8499259 1.36605218 1.27554429 0.72559583 0.6084000 0.8473667 0.23896667 0.02520281 0.02456591 FALSE TRUE
ENST00000345392 ENSG00000134086 HEK293_OSMI2_2hA HEK293_TMG_2hB VHL protein_coding protein_coding 43.79489 35.36838 58.70634 5.744034 0.9190629 0.7308938 2.876857 3.492080 3.42484024 0.3263392 0.14670564 -0.02796897 0.06800833 0.1020000 0.0584000 -0.04360000 0.03525864 0.02456591 FALSE TRUE
ENST00000477538 ENSG00000134086 HEK293_OSMI2_2hA HEK293_TMG_2hB VHL protein_coding processed_transcript 43.79489 35.36838 58.70634 5.744034 0.9190629 0.7308938 4.473816 3.630204 3.31949164 0.1056361 0.35189531 -0.12871729 0.10907083 0.1066333 0.0566000 -0.05003333 0.02456591 0.02456591   FALSE
MSTRG.22456.1 ENSG00000134086 HEK293_OSMI2_2hA HEK293_TMG_2hB VHL protein_coding   43.79489 35.36838 58.70634 5.744034 0.9190629 0.7308938 2.785037 7.132615 0.04554169 5.4328325 0.04554169 -7.00673747 0.07392500 0.1674333 0.0008000 -0.16663333 0.22094758 0.02456591 FALSE FALSE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000134086 E001 0.8073328 0.015629270 9.889408e-01 9.971146e-01 3 10134889 10134926 38 + 0.226 0.286 0.452
ENSG00000134086 E002 6.5795042 0.015202044 2.657659e-01 4.038546e-01 3 10141778 10141785 8 + 0.880 0.848 -0.123
ENSG00000134086 E003 6.5815805 0.008428888 2.400943e-01 3.742603e-01 3 10141786 10141787 2 + 0.880 0.849 -0.122
ENSG00000134086 E004 143.5021347 0.008115289 4.559553e-10 7.192885e-09 3 10141788 10141847 60 + 2.214 1.998 -0.723
ENSG00000134086 E005 452.9109387 0.011379607 1.126785e-04 5.802287e-04 3 10141848 10142187 340 + 2.672 2.588 -0.279
ENSG00000134086 E006 16.4689945 0.003178801 7.489785e-02 1.513657e-01 3 10142546 10142762 217 + 1.108 1.393 1.005
ENSG00000134086 E007 61.8714354 0.002343294 2.715554e-01 4.103285e-01 3 10142763 10143021 259 + 1.706 1.896 0.643
ENSG00000134086 E008 349.6626963 0.002127186 1.269104e-12 3.061217e-11 3 10146514 10146636 123 + 2.559 2.483 -0.251
ENSG00000134086 E009 2.0850178 0.053002117 8.993715e-01 9.397914e-01 3 10148704 10148814 111 + 0.450 0.522 0.354
ENSG00000134086 E010 4680.4904543 0.008691969 1.092823e-06 8.997482e-06 3 10149787 10153667 3881 + 3.584 3.755 0.569