ENSG00000133935

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000256319 ENSG00000133935 HEK293_OSMI2_2hA HEK293_TMG_2hB ERG28 protein_coding protein_coding 76.97424 126.8422 42.50994 5.668335 0.3952513 -1.576936 14.04859 20.11306 10.08467 1.277183 0.1424943 -0.9952555 0.1933458 0.1583333 0.2373333 0.07900000 7.706483e-07 7.706483e-07 FALSE  
MSTRG.9946.2 ENSG00000133935 HEK293_OSMI2_2hA HEK293_TMG_2hB ERG28 protein_coding   76.97424 126.8422 42.50994 5.668335 0.3952513 -1.576936 61.38018 104.60542 31.33377 4.210933 0.9027535 -1.7388451 0.7875250 0.8250000 0.7368667 -0.08813333 4.356096e-04 7.706483e-07    
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000133935 E001 28.67613 0.0103833284 4.800133e-03 1.537398e-02 14 75649791 75650903 1113 - 1.551 1.339 -0.734
ENSG00000133935 E002 569.41716 0.0028340808 4.736449e-04 2.059327e-03 14 75650904 75651058 155 - 2.571 2.700 0.428
ENSG00000133935 E003 2317.56103 0.0011119702 2.519197e-09 3.467913e-08 14 75651059 75651634 576 - 3.203 3.303 0.331
ENSG00000133935 E004 991.83219 0.0003627935 4.919915e-03 1.570716e-02 14 75651771 75651889 119 - 2.926 2.909 -0.059
ENSG00000133935 E005 28.09495 0.0029128211 6.258129e-02 1.309965e-01 14 75654882 75654885 4 - 1.469 1.348 -0.419
ENSG00000133935 E006 850.07704 0.0005408618 4.183974e-07 3.758910e-06 14 75654886 75654976 91 - 2.892 2.833 -0.197
ENSG00000133935 E007 865.03062 0.0007614940 2.864625e-06 2.153123e-05 14 75657370 75657533 164 - 2.898 2.840 -0.192
ENSG00000133935 E008 381.52658 0.0021536124 3.076940e-02 7.346336e-02 14 75660775 75661006 232 - 2.530 2.490 -0.133