ENSG00000133858

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000378743 ENSG00000133858 HEK293_OSMI2_2hA HEK293_TMG_2hB ZFC3H1 protein_coding protein_coding 10.60963 4.283268 14.34739 0.6927684 0.6056005 1.741645 6.42588896 2.44991590 9.3955168 0.37356662 0.2411060 1.93489823 0.60140417 0.57490000 0.65596667 0.08106667 2.933530e-01 4.951495e-05 FALSE TRUE
ENST00000547398 ENSG00000133858 HEK293_OSMI2_2hA HEK293_TMG_2hB ZFC3H1 protein_coding retained_intron 10.60963 4.283268 14.34739 0.6927684 0.6056005 1.741645 0.03600234 0.28801874 0.0000000 0.28801874 0.0000000 -4.89733116 0.00635000 0.05080000 0.00000000 -0.05080000 5.615385e-01 4.951495e-05   FALSE
ENST00000550963 ENSG00000133858 HEK293_OSMI2_2hA HEK293_TMG_2hB ZFC3H1 protein_coding retained_intron 10.60963 4.283268 14.34739 0.6927684 0.6056005 1.741645 1.55040669 1.19986492 1.2645916 0.18889452 0.3472517 0.07518911 0.18205417 0.29080000 0.08660000 -0.20420000 1.506758e-02 4.951495e-05   FALSE
ENST00000551487 ENSG00000133858 HEK293_OSMI2_2hA HEK293_TMG_2hB ZFC3H1 protein_coding retained_intron 10.60963 4.283268 14.34739 0.6927684 0.6056005 1.741645 0.60744193 0.11074855 0.8498781 0.02159117 0.2940385 2.83212623 0.05214167 0.02633333 0.05890000 0.03256667 5.132779e-01 4.951495e-05   FALSE
ENST00000552994 ENSG00000133858 HEK293_OSMI2_2hA HEK293_TMG_2hB ZFC3H1 protein_coding nonsense_mediated_decay 10.60963 4.283268 14.34739 0.6927684 0.6056005 1.741645 1.03023359 0.14071231 1.2231361 0.05844917 0.1347412 3.03246283 0.09218333 0.03733333 0.08633333 0.04900000 1.935829e-01 4.951495e-05 TRUE TRUE
MSTRG.7662.3 ENSG00000133858 HEK293_OSMI2_2hA HEK293_TMG_2hB ZFC3H1 protein_coding   10.60963 4.283268 14.34739 0.6927684 0.6056005 1.741645 0.62499829 0.01980096 1.2283769 0.01980096 0.2213321 5.37694811 0.04000417 0.00550000 0.08513333 0.07963333 4.951495e-05 4.951495e-05 FALSE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000133858 E001 0.6695470 0.0234668686 2.378391e-02 5.937244e-02 12 71609599 71609697 99 - 0.065 0.472 3.603
ENSG00000133858 E002 123.7270970 0.0297754373 2.468295e-09 3.402540e-08 12 71609698 71610109 412 - 1.809 2.382 1.921
ENSG00000133858 E003 124.9530018 0.0192933995 3.925592e-09 5.213766e-08 12 71610110 71610387 278 - 1.863 2.337 1.588
ENSG00000133858 E004 100.2136419 0.0024630361 5.547151e-16 2.168472e-14 12 71610388 71610565 178 - 1.803 2.192 1.306
ENSG00000133858 E005 15.2355518 0.0018471019 3.186051e-03 1.081524e-02 12 71610566 71610694 129 - 1.042 1.355 1.111
ENSG00000133858 E006 66.4104327 0.0004515576 2.515356e-15 9.028547e-14 12 71610695 71610757 63 - 1.620 2.022 1.357
ENSG00000133858 E007 52.0104243 0.0004749357 4.915431e-12 1.076548e-10 12 71611058 71611097 40 - 1.521 1.915 1.338
ENSG00000133858 E008 25.1820327 0.0007420812 7.150684e-04 2.952585e-03 12 71611098 71611555 458 - 1.254 1.545 1.007
ENSG00000133858 E009 10.9565405 0.0104602952 6.212808e-01 7.382522e-01 12 71611556 71611785 230 - 0.988 1.084 0.352
ENSG00000133858 E010 76.0515443 0.0003802584 9.346355e-11 1.657126e-09 12 71611786 71611887 102 - 1.711 2.033 1.084
ENSG00000133858 E011 48.0756933 0.0004397406 9.756371e-04 3.876500e-03 12 71613335 71613366 32 - 1.549 1.774 0.762
ENSG00000133858 E012 51.8309518 0.0004493506 4.829094e-03 1.545550e-02 12 71613367 71613435 69 - 1.589 1.783 0.659
ENSG00000133858 E013 5.1138356 0.0189798782 3.992688e-01 5.429988e-01 12 71613436 71613748 313 - 0.742 0.632 -0.459
ENSG00000133858 E014 73.1733875 0.0004224708 3.491426e-03 1.170028e-02 12 71614535 71614700 166 - 1.743 1.919 0.595
ENSG00000133858 E015 57.9326721 0.0004797397 6.882391e-02 1.414225e-01 12 71614834 71614938 105 - 1.656 1.792 0.462
ENSG00000133858 E016 0.5975289 0.0278467965 3.711478e-01 5.155542e-01 12 71614939 71615070 132 - 0.217 0.000 -10.134
ENSG00000133858 E017 0.4396707 0.0316418887 5.491764e-01 6.789427e-01 12 71615071 71615205 135 - 0.172 0.000 -9.718
ENSG00000133858 E018 68.1040441 0.0004034917 3.525603e-01 4.968163e-01 12 71615206 71615316 111 - 1.736 1.823 0.295
ENSG00000133858 E019 57.7689676 0.0004261389 3.756071e-01 5.200290e-01 12 71619315 71619409 95 - 1.692 1.683 -0.028
ENSG00000133858 E020 70.7488934 0.0003895523 4.720368e-01 6.111173e-01 12 71619926 71620124 199 - 1.760 1.836 0.256
ENSG00000133858 E021 61.5311499 0.0003852158 2.189845e-01 3.491643e-01 12 71620210 71620315 106 - 1.693 1.797 0.351
ENSG00000133858 E022 80.3720654 0.0003267767 4.690083e-01 6.083185e-01 12 71623360 71623597 238 - 1.813 1.887 0.250
ENSG00000133858 E023 58.3121737 0.0004728639 2.595293e-01 3.969342e-01 12 71624104 71624292 189 - 1.668 1.769 0.341
ENSG00000133858 E024 70.4122312 0.0124789264 9.021995e-01 9.416765e-01 12 71626260 71626446 187 - 1.760 1.819 0.201
ENSG00000133858 E025 71.5393059 0.0287794420 2.571604e-01 3.942086e-01 12 71627751 71627934 184 - 1.791 1.750 -0.138
ENSG00000133858 E026 61.1907612 0.0347432771 2.239165e-01 3.550887e-01 12 71628918 71629037 120 - 1.730 1.668 -0.209
ENSG00000133858 E027 54.6740374 0.0034708842 8.546037e-02 1.681488e-01 12 71629609 71629710 102 - 1.683 1.614 -0.233
ENSG00000133858 E028 61.0731072 0.0018385241 1.741029e-01 2.934958e-01 12 71630600 71630721 122 - 1.721 1.684 -0.123
ENSG00000133858 E029 60.1297753 0.0038672104 3.539037e-01 4.981832e-01 12 71630823 71630954 132 - 1.711 1.696 -0.050
ENSG00000133858 E030 49.1619571 0.0019202811 4.619971e-02 1.024031e-01 12 71631778 71631887 110 - 1.642 1.554 -0.299
ENSG00000133858 E031 71.0438058 0.0003464676 4.324419e-03 1.405373e-02 12 71631972 71632222 251 - 1.803 1.695 -0.366
ENSG00000133858 E032 75.2316452 0.0003270852 1.039481e-04 5.402797e-04 12 71632223 71632514 292 - 1.838 1.677 -0.544
ENSG00000133858 E033 47.3218330 0.0004486664 3.418928e-06 2.527928e-05 12 71632886 71633017 132 - 1.659 1.379 -0.959
ENSG00000133858 E034 0.5181333 0.0201571685 6.133580e-01 7.320488e-01 12 71633018 71633091 74 - 0.122 0.219 1.022
ENSG00000133858 E035 43.9399472 0.0005256786 9.964995e-05 5.201839e-04 12 71633264 71633438 175 - 1.622 1.391 -0.793
ENSG00000133858 E036 50.9238671 0.0004753056 6.677654e-08 7.017982e-07 12 71634155 71634304 150 - 1.696 1.367 -1.125
ENSG00000133858 E037 50.2806293 0.0022274040 5.576133e-07 4.889297e-06 12 71634704 71634825 122 - 1.687 1.368 -1.093
ENSG00000133858 E038 46.5879760 0.0050881357 2.214563e-08 2.560996e-07 12 71635443 71635580 138 - 1.668 1.241 -1.472
ENSG00000133858 E039 58.1204962 0.0006230283 2.830750e-11 5.476126e-10 12 71636490 71636654 165 - 1.761 1.355 -1.390
ENSG00000133858 E040 72.3594043 0.0037538825 2.223420e-14 6.949683e-13 12 71636850 71637059 210 - 1.865 1.367 -1.697
ENSG00000133858 E041 43.2668528 0.0005371973 6.539102e-12 1.402960e-10 12 71638418 71638515 98 - 1.648 1.129 -1.803
ENSG00000133858 E042 42.4748058 0.0004949971 1.735984e-11 3.473564e-10 12 71642436 71642559 124 - 1.638 1.129 -1.771
ENSG00000133858 E043 75.5408165 0.0037346024 3.420415e-11 6.521745e-10 12 71644095 71644318 224 - 1.872 1.476 -1.345
ENSG00000133858 E044 39.9026228 0.0005421294 1.029502e-12 2.522779e-11 12 71644877 71644913 37 - 1.619 1.035 -2.044
ENSG00000133858 E045 60.5591784 0.0042110694 3.717647e-11 7.047199e-10 12 71644914 71645075 162 - 1.784 1.330 -1.552
ENSG00000133858 E046 30.3966185 0.0021103580 9.911130e-04 3.927858e-03 12 71647749 71647813 65 - 1.466 1.224 -0.844
ENSG00000133858 E047 0.4438354 0.2608570210 1.421015e-01 2.508971e-01 12 71656343 71656563 221 - 0.065 0.357 2.983
ENSG00000133858 E048 0.5922303 0.0180036588 8.572058e-01 9.118093e-01 12 71656564 71656882 319 - 0.172 0.219 0.434
ENSG00000133858 E049 0.2924217 0.0273604260 1.000000e+00   12 71656883 71656884 2 - 0.122 0.000 -9.133
ENSG00000133858 E050 73.5593879 0.0013740965 3.617014e-12 8.092977e-11 12 71656885 71657301 417 - 1.860 1.475 -1.307
ENSG00000133858 E051 0.9504292 0.3759999278 4.639659e-01 6.037168e-01 12 71660289 71660370 82 - 0.215 0.365 1.036
ENSG00000133858 E052 0.4386386 0.2107397108 6.145182e-01 7.329526e-01 12 71660371 71660427 57 - 0.121 0.217 1.007
ENSG00000133858 E053 0.3268771 0.0295810609 3.416882e-01   12 71662985 71663012 28 - 0.065 0.219 2.021
ENSG00000133858 E054 78.3626409 0.0023998788 1.325344e-13 3.703826e-12 12 71663013 71663858 846 - 1.892 1.466 -1.446
ENSG00000133858 E055 0.0000000       12 71667521 71667725 205 -