ENSG00000133740

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000416274 ENSG00000133740 HEK293_OSMI2_2hA HEK293_TMG_2hB E2F5 protein_coding protein_coding 15.91393 10.00093 24.85397 1.474477 1.027297 1.312481 2.298988 1.161548 4.658343 0.3181075 0.3158679 1.9944950 0.1487000 0.1200667 0.18710000 0.06703333 3.222944e-01 1.124793e-12 FALSE TRUE
ENST00000418930 ENSG00000133740 HEK293_OSMI2_2hA HEK293_TMG_2hB E2F5 protein_coding protein_coding 15.91393 10.00093 24.85397 1.474477 1.027297 1.312481 2.151126 2.594601 1.696988 0.7171918 0.3754551 -0.6096097 0.1542875 0.2524333 0.06883333 -0.18360000 7.256596e-04 1.124793e-12 FALSE TRUE
ENST00000517476 ENSG00000133740 HEK293_OSMI2_2hA HEK293_TMG_2hB E2F5 protein_coding protein_coding 15.91393 10.00093 24.85397 1.474477 1.027297 1.312481 9.619744 5.992704 13.901897 0.6718059 1.1087389 1.2126347 0.6010667 0.6049000 0.55790000 -0.04700000 6.497566e-01 1.124793e-12 FALSE TRUE
MSTRG.31756.2 ENSG00000133740 HEK293_OSMI2_2hA HEK293_TMG_2hB E2F5 protein_coding   15.91393 10.00093 24.85397 1.474477 1.027297 1.312481 1.404989 0.000000 4.140503 0.0000000 0.8334700 8.6971423 0.0591250 0.0000000 0.16780000 0.16780000 1.124793e-12 1.124793e-12 FALSE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000133740 E001 12.5865882 0.0024615533 1.704056e-03 6.295648e-03 8 85177154 85177224 71 + 1.217 0.891 -1.191
ENSG00000133740 E002 56.6269447 0.0031976257 2.250866e-15 8.123830e-14 8 85177225 85177611 387 + 1.881 1.397 -1.649
ENSG00000133740 E003 52.0976820 0.0004816437 1.130533e-06 9.281335e-06 8 85177612 85177654 43 + 1.795 1.548 -0.840
ENSG00000133740 E004 0.2998086 0.0279401406 6.449627e-01   8 85187685 85187796 112 + 0.091 0.162 0.943
ENSG00000133740 E005 1.2564787 0.0200063515 1.248139e-01 2.268594e-01 8 85201785 85202146 362 + 0.231 0.512 1.678
ENSG00000133740 E006 104.9590897 0.0028558188 2.794628e-04 1.296203e-03 8 85202147 85202256 110 + 2.070 1.920 -0.503
ENSG00000133740 E007 142.5530983 0.0003934541 1.658735e-02 4.403187e-02 8 85203094 85203255 162 + 2.174 2.111 -0.210
ENSG00000133740 E008 0.0000000       8 85203256 85203262 7 +      
ENSG00000133740 E009 1.4780283 0.0091267650 7.303639e-02 1.483873e-01 8 85206163 85206176 14 + 0.493 0.162 -2.225
ENSG00000133740 E010 86.8263650 0.0003622601 3.088250e-01 4.510923e-01 8 85206177 85206220 44 + 1.944 1.915 -0.098
ENSG00000133740 E011 0.7447899 0.0264568020 8.222200e-01 8.879008e-01 8 85206221 85206224 4 + 0.231 0.279 0.356
ENSG00000133740 E012 105.6657576 0.0011918902 5.277760e-01 6.607734e-01 8 85207425 85207489 65 + 2.024 2.007 -0.055
ENSG00000133740 E013 114.5596443 0.0003314876 3.810560e-01 5.253461e-01 8 85209142 85209212 71 + 2.061 2.042 -0.064
ENSG00000133740 E014 218.9316742 0.0002082737 1.800353e-01 3.012201e-01 8 85209213 85209409 197 + 2.342 2.321 -0.071
ENSG00000133740 E015 40.1223859 0.0005459381 7.302025e-01 8.222363e-01 8 85212157 85212159 3 + 1.609 1.600 -0.029
ENSG00000133740 E016 102.0320741 0.0003390469 6.425307e-01 7.552944e-01 8 85212160 85212204 45 + 2.006 2.000 -0.021
ENSG00000133740 E017 6.9219350 0.0030033504 5.994320e-01 7.208184e-01 8 85212205 85213752 1548 + 0.917 0.864 -0.203
ENSG00000133740 E018 173.1831435 0.0002829819 6.815366e-01 7.851899e-01 8 85213753 85213902 150 + 2.226 2.248 0.074
ENSG00000133740 E019 234.0784091 0.0007762437 2.406122e-08 2.760614e-07 8 85213903 85214159 257 + 2.303 2.452 0.496
ENSG00000133740 E020 255.8248752 0.0022559502 7.282760e-10 1.108190e-08 8 85214160 85216124 1965 + 2.321 2.510 0.631
ENSG00000133740 E021 1.5792043 0.0429967720 7.260489e-01 8.191878e-01 8 85216125 85216307 183 + 0.381 0.448 0.360
ENSG00000133740 E022 0.1817044 0.0465454255 2.567146e-01   8 85216960 85217158 199 + 0.000 0.162 10.095