ENSG00000133731

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000256108 ENSG00000133731 HEK293_OSMI2_2hA HEK293_TMG_2hB IMPA1 protein_coding protein_coding 16.63507 4.369849 26.12914 0.5329286 1.266699 2.577258 4.9174081 2.77180704 7.0094120 0.55078315 0.1727738 1.3353278 0.38700417 0.62343333 0.26910000 -0.35433333 5.945866e-08 1.067866e-09 FALSE TRUE
ENST00000311489 ENSG00000133731 HEK293_OSMI2_2hA HEK293_TMG_2hB IMPA1 protein_coding protein_coding 16.63507 4.369849 26.12914 0.5329286 1.266699 2.577258 3.2922811 0.36550556 4.9032548 0.18748818 0.9978205 3.7097729 0.15018750 0.08803333 0.18650000 0.09846667 4.849290e-01 1.067866e-09 FALSE TRUE
ENST00000449740 ENSG00000133731 HEK293_OSMI2_2hA HEK293_TMG_2hB IMPA1 protein_coding protein_coding 16.63507 4.369849 26.12914 0.5329286 1.266699 2.577258 0.7971530 0.09481470 2.1390606 0.06961893 0.3743976 4.3577932 0.04080000 0.02163333 0.08140000 0.05976667 1.228214e-01 1.067866e-09 FALSE TRUE
ENST00000518202 ENSG00000133731 HEK293_OSMI2_2hA HEK293_TMG_2hB IMPA1 protein_coding protein_coding 16.63507 4.369849 26.12914 0.5329286 1.266699 2.577258 0.6981629 0.60590308 0.7930965 0.14747300 0.3849038 0.3828699 0.08182083 0.14270000 0.03006667 -0.11263333 5.069958e-02 1.067866e-09 FALSE FALSE
ENST00000519816 ENSG00000133731 HEK293_OSMI2_2hA HEK293_TMG_2hB IMPA1 protein_coding nonsense_mediated_decay 16.63507 4.369849 26.12914 0.5329286 1.266699 2.577258 1.0150113 0.26803062 2.0372686 0.15705610 0.1863337 2.8803847 0.06049167 0.05926667 0.07773333 0.01846667 7.209100e-01 1.067866e-09 TRUE TRUE
ENST00000520065 ENSG00000133731 HEK293_OSMI2_2hA HEK293_TMG_2hB IMPA1 protein_coding retained_intron 16.63507 4.369849 26.12914 0.5329286 1.266699 2.577258 1.1413184 0.08637058 1.4368403 0.04943274 0.4146784 3.9081692 0.08797083 0.02310000 0.05403333 0.03093333 3.144856e-01 1.067866e-09   FALSE
MSTRG.31737.3 ENSG00000133731 HEK293_OSMI2_2hA HEK293_TMG_2hB IMPA1 protein_coding   16.63507 4.369849 26.12914 0.5329286 1.266699 2.577258 3.3222861 0.00000000 5.7616025 0.00000000 1.0701705 9.1728281 0.12463750 0.00000000 0.22376667 0.22376667 1.067866e-09 1.067866e-09 FALSE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000133731 E001 261.589897 0.0141913959 9.386836e-11 1.663992e-09 8 81656914 81659043 2130 - 2.199 2.620 1.402
ENSG00000133731 E002 60.757249 0.0004442620 9.912629e-01 9.986442e-01 8 81659044 81659148 105 - 1.678 1.755 0.262
ENSG00000133731 E003 73.076140 0.0063957693 7.616360e-01 8.454554e-01 8 81659149 81659261 113 - 1.751 1.851 0.337
ENSG00000133731 E004 115.392722 0.0010384622 3.499382e-01 4.941311e-01 8 81659262 81659466 205 - 1.957 1.995 0.126
ENSG00000133731 E005 0.000000       8 81660514 81660515 2 -      
ENSG00000133731 E006 71.977203 0.0011454117 3.036019e-02 7.267320e-02 8 81660516 81660572 57 - 1.769 1.726 -0.143
ENSG00000133731 E007 93.960152 0.0005916611 2.763790e-03 9.564192e-03 8 81660573 81660667 95 - 1.887 1.823 -0.216
ENSG00000133731 E008 38.716864 0.0011534562 2.611485e-02 6.417683e-02 8 81670939 81670939 1 - 1.516 1.427 -0.304
ENSG00000133731 E009 48.025621 0.0013522707 4.543637e-03 1.466428e-02 8 81670940 81670977 38 - 1.611 1.494 -0.402
ENSG00000133731 E010 46.285575 0.0014922835 4.551805e-03 1.468664e-02 8 81670978 81671007 30 - 1.596 1.473 -0.420
ENSG00000133731 E011 46.651936 0.0005224055 6.908330e-06 4.764160e-05 8 81671008 81671047 40 - 1.614 1.363 -0.862
ENSG00000133731 E012 46.072085 0.0006039707 5.331135e-04 2.283694e-03 8 81673841 81673867 27 - 1.596 1.428 -0.576
ENSG00000133731 E013 51.207867 0.0010138583 3.183590e-03 1.080747e-02 8 81673868 81673895 28 - 1.634 1.514 -0.408
ENSG00000133731 E014 55.245775 0.0036503254 6.495399e-03 1.991091e-02 8 81673896 81673949 54 - 1.666 1.552 -0.389
ENSG00000133731 E015 10.294829 0.0851922878 1.433052e-01 2.525127e-01 8 81673950 81674421 472 - 0.992 0.740 -0.969
ENSG00000133731 E016 10.371538 0.0018635632 3.893333e-01 5.334387e-01 8 81674672 81674831 160 - 0.964 0.922 -0.155
ENSG00000133731 E017 42.164050 0.0005385823 4.282992e-02 9.623285e-02 8 81676234 81676265 32 - 1.547 1.483 -0.218
ENSG00000133731 E018 33.676244 0.0006351675 1.516182e-01 2.638561e-01 8 81676266 81676279 14 - 1.448 1.415 -0.113
ENSG00000133731 E019 5.276720 0.0032385691 3.841611e-06 2.805989e-05 8 81678583 81678689 107 - 0.479 1.154 2.717
ENSG00000133731 E020 78.790800 0.0003884008 2.255156e-01 3.570179e-01 8 81679126 81679230 105 - 1.795 1.813 0.060
ENSG00000133731 E021 86.167713 0.0023881973 1.813607e-01 3.029533e-01 8 81680650 81680779 130 - 1.836 1.842 0.020
ENSG00000133731 E022 31.281601 0.0006924752 7.662969e-01 8.488407e-01 8 81680780 81680783 4 - 1.386 1.484 0.337
ENSG00000133731 E023 57.312926 0.0004587820 2.820558e-01 4.220144e-01 8 81681498 81681563 66 - 1.660 1.676 0.051
ENSG00000133731 E024 40.531606 0.0006411061 2.559644e-01 3.929220e-01 8 81681564 81681584 21 - 1.516 1.514 -0.007
ENSG00000133731 E025 8.899743 0.0022331010 2.489645e-02 6.166048e-02 8 81685763 81685963 201 - 0.943 0.670 -1.078
ENSG00000133731 E026 2.432623 0.0058525582 9.821470e-01 9.928857e-01 8 81685964 81686051 88 - 0.458 0.506 0.239
ENSG00000133731 E027 39.594312 0.0005916155 4.224840e-01 5.656219e-01 8 81686252 81686339 88 - 1.502 1.524 0.073