ENSG00000133619

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000319551 ENSG00000133619 HEK293_OSMI2_2hA HEK293_TMG_2hB KRBA1 protein_coding protein_coding 16.22618 22.30442 10.87455 1.52729 0.2879205 -1.035696 1.2422626 1.69368682 0.9925380 0.17823229 0.2286006 -0.7650032 0.07699167 0.076000000 0.09050000 0.014500000 8.439930e-01 2.828016e-05 FALSE TRUE
ENST00000467333 ENSG00000133619 HEK293_OSMI2_2hA HEK293_TMG_2hB KRBA1 protein_coding protein_coding 16.22618 22.30442 10.87455 1.52729 0.2879205 -1.035696 2.0897333 3.88178367 0.9960171 0.22018798 0.1330273 -1.9517767 0.12140000 0.176933333 0.09113333 -0.085800000 2.261372e-02 2.828016e-05 FALSE FALSE
ENST00000496080 ENSG00000133619 HEK293_OSMI2_2hA HEK293_TMG_2hB KRBA1 protein_coding retained_intron 16.22618 22.30442 10.87455 1.52729 0.2879205 -1.035696 0.7271477 0.03528739 1.1391014 0.03528739 0.3445698 4.6652531 0.05555417 0.001633333 0.10610000 0.104466667 2.828016e-05 2.828016e-05 FALSE FALSE
ENST00000496259 ENSG00000133619 HEK293_OSMI2_2hA HEK293_TMG_2hB KRBA1 protein_coding protein_coding 16.22618 22.30442 10.87455 1.52729 0.2879205 -1.035696 3.5770205 4.88422816 2.1315588 0.47531552 0.3353566 -1.1924201 0.21558333 0.218166667 0.19790000 -0.020266667 8.290132e-01 2.828016e-05 FALSE TRUE
ENST00000497895 ENSG00000133619 HEK293_OSMI2_2hA HEK293_TMG_2hB KRBA1 protein_coding protein_coding 16.22618 22.30442 10.87455 1.52729 0.2879205 -1.035696 2.1910434 2.14145956 1.4954831 1.01417738 0.2172548 -0.5150893 0.14077917 0.091200000 0.13676667 0.045566667 5.043818e-01 2.828016e-05 FALSE FALSE
MSTRG.30907.10 ENSG00000133619 HEK293_OSMI2_2hA HEK293_TMG_2hB KRBA1 protein_coding   16.22618 22.30442 10.87455 1.52729 0.2879205 -1.035696 1.2893234 2.00692389 1.0466796 0.23767318 0.3060318 -0.9326187 0.07450417 0.089466667 0.09620000 0.006733333 1.000000e+00 2.828016e-05 FALSE TRUE
MSTRG.30907.3 ENSG00000133619 HEK293_OSMI2_2hA HEK293_TMG_2hB KRBA1 protein_coding   16.22618 22.30442 10.87455 1.52729 0.2879205 -1.035696 1.0058560 1.87511279 0.7916749 0.48912086 0.5024259 -1.2335615 0.05723333 0.082966667 0.07530000 -0.007666667 8.216341e-01 2.828016e-05 TRUE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000133619 E001 0.0000000       7 149714781 149714941 161 +      
ENSG00000133619 E002 0.0000000       7 149714942 149714943 2 +      
ENSG00000133619 E003 0.3289534 0.0292644589 5.053984e-01   7 149714944 149714950 7 + 0.177 0.083 -1.266
ENSG00000133619 E004 0.9858280 0.0612925375 8.074566e-01 8.776659e-01 7 149714951 149714957 7 + 0.303 0.264 -0.268
ENSG00000133619 E005 0.9858280 0.0612925375 8.074566e-01 8.776659e-01 7 149714958 149714958 1 + 0.303 0.264 -0.268
ENSG00000133619 E006 0.9858280 0.0612925375 8.074566e-01 8.776659e-01 7 149714959 149714963 5 + 0.303 0.264 -0.268
ENSG00000133619 E007 3.0821189 0.0298846370 3.869932e-01 5.311505e-01 7 149714964 149714967 4 + 0.656 0.521 -0.607
ENSG00000133619 E008 46.0736107 0.0005504016 3.069566e-03 1.047105e-02 7 149714968 149715056 89 + 1.716 1.569 -0.498
ENSG00000133619 E009 41.9845604 0.0005416863 2.078095e-03 7.466270e-03 7 149715057 149715066 10 + 1.685 1.525 -0.545
ENSG00000133619 E010 15.8924014 0.0017952419 2.009295e-03 7.255177e-03 7 149715067 149715238 172 + 1.346 1.091 -0.904
ENSG00000133619 E011 16.0532902 0.0121110495 5.045838e-02 1.099819e-01 7 149715239 149715349 111 + 1.305 1.125 -0.637
ENSG00000133619 E012 11.1070890 0.0466233050 6.050724e-01 7.254941e-01 7 149719422 149719532 111 + 1.045 1.004 -0.153
ENSG00000133619 E013 19.7528750 0.0042485092 1.600301e-01 2.751471e-01 7 149719533 149719545 13 + 1.326 1.220 -0.373
ENSG00000133619 E014 63.6333723 0.0013449396 1.368224e-01 2.436613e-01 7 149719546 149719638 93 + 1.789 1.731 -0.199
ENSG00000133619 E015 45.2600299 0.0005107271 7.609755e-01 8.449302e-01 7 149719639 149719672 34 + 1.578 1.612 0.114
ENSG00000133619 E016 53.3438569 0.0028589401 9.403999e-01 9.665841e-01 7 149720008 149720052 45 + 1.662 1.673 0.039
ENSG00000133619 E017 73.2721765 0.0003743269 1.259732e-01 2.285353e-01 7 149720053 149720100 48 + 1.846 1.793 -0.179
ENSG00000133619 E018 55.7346900 0.0004197511 5.655798e-01 6.926461e-01 7 149720101 149720127 27 + 1.703 1.689 -0.048
ENSG00000133619 E019 91.5458187 0.0009910083 5.606650e-01 6.886296e-01 7 149720835 149720949 115 + 1.913 1.904 -0.029
ENSG00000133619 E020 63.5923355 0.0004379960 4.888457e-01 6.263635e-01 7 149720950 149720990 41 + 1.707 1.758 0.171
ENSG00000133619 E021 17.5724410 0.0010251282 3.579470e-01 5.023495e-01 7 149721380 149721382 3 + 1.134 1.234 0.356
ENSG00000133619 E022 85.2721563 0.0060328750 4.177042e-01 5.610306e-01 7 149721383 149721535 153 + 1.830 1.894 0.214
ENSG00000133619 E023 38.9799992 0.0083180169 2.273809e-03 8.071167e-03 7 149721536 149722040 505 + 1.336 1.602 0.915
ENSG00000133619 E024 55.5621952 0.0005408001 1.364012e-02 3.740433e-02 7 149722422 149722553 132 + 1.771 1.662 -0.370
ENSG00000133619 E025 91.6018472 0.0003020833 4.432862e-02 9.898542e-02 7 149722783 149722956 174 + 1.951 1.886 -0.217
ENSG00000133619 E026 4.7424233 0.0730068968 8.601304e-06 5.801998e-05 7 149723561 149723733 173 + 1.119 0.357 -3.252
ENSG00000133619 E027 103.4381401 0.0003507683 3.417027e-03 1.148835e-02 7 149723734 149723889 156 + 2.023 1.929 -0.315
ENSG00000133619 E028 106.4507341 0.0007884099 9.975638e-02 1.901071e-01 7 149724652 149724849 198 + 2.004 1.957 -0.159
ENSG00000133619 E029 123.3426072 0.0002686614 1.769326e-01 2.972469e-01 7 149725315 149725488 174 + 2.055 2.024 -0.104
ENSG00000133619 E030 126.0761025 0.0002579118 3.452113e-01 4.892956e-01 7 149725887 149726060 174 + 2.055 2.039 -0.056
ENSG00000133619 E031 110.9510403 0.0017592138 8.201853e-01 8.865476e-01 7 149728523 149728675 153 + 1.982 1.989 0.023
ENSG00000133619 E032 77.4740764 0.0010765759 1.416885e-01 2.503447e-01 7 149729188 149729370 183 + 1.764 1.850 0.292
ENSG00000133619 E033 6.1289030 0.0047177287 6.418848e-02 1.336768e-01 7 149730040 149730146 107 + 0.958 0.733 -0.875
ENSG00000133619 E034 94.8610617 0.0008643378 9.840056e-03 2.837094e-02 7 149730147 149730341 195 + 1.820 1.949 0.435
ENSG00000133619 E035 85.5587366 0.0003544083 1.074856e-01 2.018299e-01 7 149730428 149730545 118 + 1.807 1.894 0.292
ENSG00000133619 E036 42.3559136 0.0088399901 1.597619e-01 2.747995e-01 7 149730546 149730568 23 + 1.481 1.606 0.425
ENSG00000133619 E037 8.2727700 0.0024899703 6.944365e-01 7.952715e-01 7 149730569 149730713 145 + 0.933 0.899 -0.128
ENSG00000133619 E038 97.8884249 0.0003311912 3.065944e-01 4.487707e-01 7 149731672 149731776 105 + 1.891 1.948 0.193
ENSG00000133619 E039 552.3300719 0.0041030935 9.622801e-04 3.829432e-03 7 149733206 149734575 1370 + 2.590 2.710 0.400