ENSG00000133424

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000354992 ENSG00000133424 HEK293_OSMI2_2hA HEK293_TMG_2hB LARGE1 protein_coding protein_coding 15.66216 20.71635 12.73642 1.15248 0.4029666 -0.701374 1.4391882 1.871787 1.6710103 0.74411242 0.2536740 -0.1627747 0.08937083 0.08833333 0.13040000 0.04206667 0.54075397 0.03892322 FALSE  
ENST00000397394 ENSG00000133424 HEK293_OSMI2_2hA HEK293_TMG_2hB LARGE1 protein_coding protein_coding 15.66216 20.71635 12.73642 1.15248 0.4029666 -0.701374 1.1355246 1.412979 1.0581885 0.15014898 0.1740865 -0.4137478 0.07337917 0.06840000 0.08326667 0.01486667 0.74166853 0.03892322 FALSE  
ENST00000402320 ENSG00000133424 HEK293_OSMI2_2hA HEK293_TMG_2hB LARGE1 protein_coding protein_coding 15.66216 20.71635 12.73642 1.15248 0.4029666 -0.701374 2.9940789 2.782730 2.4057547 0.60479758 0.1673607 -0.2092023 0.19330417 0.13213333 0.18983333 0.05770000 0.37259885 0.03892322 FALSE  
ENST00000674668 ENSG00000133424 HEK293_OSMI2_2hA HEK293_TMG_2hB LARGE1 protein_coding protein_coding 15.66216 20.71635 12.73642 1.15248 0.4029666 -0.701374 0.7218351 2.381595 0.3233257 0.52072793 0.3233257 -2.8429686 0.03857083 0.11870000 0.02433333 -0.09436667 0.12573612 0.03892322 FALSE  
ENST00000674780 ENSG00000133424 HEK293_OSMI2_2hA HEK293_TMG_2hB LARGE1 protein_coding protein_coding 15.66216 20.71635 12.73642 1.15248 0.4029666 -0.701374 3.6710836 8.087514 1.1100439 1.09727460 0.5753417 -2.8539238 0.20877500 0.39363333 0.08440000 -0.30923333 0.23204251 0.03892322 FALSE  
ENST00000675382 ENSG00000133424 HEK293_OSMI2_2hA HEK293_TMG_2hB LARGE1 protein_coding nonsense_mediated_decay 15.66216 20.71635 12.73642 1.15248 0.4029666 -0.701374 0.6043066 2.230855 0.1490510 0.07800653 0.1490510 -3.8164873 0.03489167 0.10853333 0.01150000 -0.09703333 0.03892322 0.03892322 TRUE  
ENST00000675416 ENSG00000133424 HEK293_OSMI2_2hA HEK293_TMG_2hB LARGE1 protein_coding protein_coding 15.66216 20.71635 12.73642 1.15248 0.4029666 -0.701374 0.4619770 0.000000 2.8786914 0.00000000 1.6147156 8.1742723 0.03555417 0.00000000 0.22873333 0.22873333 0.15126453 0.03892322 FALSE  
ENST00000676132 ENSG00000133424 HEK293_OSMI2_2hA HEK293_TMG_2hB LARGE1 protein_coding protein_coding 15.66216 20.71635 12.73642 1.15248 0.4029666 -0.701374 3.1085900 0.000000 2.2166890 0.00000000 1.1886588 7.7987563 0.22722500 0.00000000 0.17396667 0.17396667 0.14730815 0.03892322 FALSE  
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000133424 E001 9.5870518 0.0019064129 4.289400e-01 5.716715e-01 22 33162226 33164364 2139 - 1.038 0.980 -0.210
ENSG00000133424 E002 3.1116058 0.0377279998 1.261210e-01 2.287551e-01 22 33164365 33166386 2022 - 0.729 0.497 -1.024
ENSG00000133424 E003 0.4438354 0.1644654307 8.958420e-01 9.374424e-01 22 33166387 33166635 249 - 0.145 0.183 0.408
ENSG00000133424 E004 1.7724351 0.0081368747 6.485132e-01 7.599972e-01 22 33166636 33166832 197 - 0.474 0.415 -0.305
ENSG00000133424 E005 0.1451727 0.0432953385 3.141294e-01   22 33210726 33210828 103 - 0.145 0.000 -12.803
ENSG00000133424 E006 0.0000000       22 33221602 33221632 31 -      
ENSG00000133424 E007 0.0000000       22 33221633 33221751 119 -      
ENSG00000133424 E008 15.4850667 0.0103740502 5.090819e-01 6.444253e-01 22 33272509 33273076 568 - 1.136 1.231 0.339
ENSG00000133424 E009 677.4563517 0.0046380324 6.071991e-11 1.109063e-09 22 33273077 33274219 1143 - 2.654 2.881 0.755
ENSG00000133424 E010 162.0815975 0.0011942142 1.319613e-01 2.368424e-01 22 33274220 33274384 165 - 2.139 2.219 0.267
ENSG00000133424 E011 188.8760660 0.0018561900 9.103896e-01 9.470258e-01 22 33274385 33274624 240 - 2.238 2.273 0.118
ENSG00000133424 E012 180.5834647 0.0020182414 8.401871e-01 9.002066e-01 22 33277060 33277255 196 - 2.222 2.247 0.084
ENSG00000133424 E013 148.3802679 0.0002360683 8.826875e-02 1.724797e-01 22 33283202 33283348 147 - 2.164 2.144 -0.068
ENSG00000133424 E014 1.5886398 0.0101846995 7.440290e-01 8.325163e-01 22 33291969 33293173 1205 - 0.340 0.416 0.434
ENSG00000133424 E015 170.2204028 0.0005486890 2.178793e-02 5.523424e-02 22 33304229 33304507 279 - 2.234 2.198 -0.118
ENSG00000133424 E016 0.0000000       22 33305532 33305625 94 -      
ENSG00000133424 E017 117.0103615 0.0006194786 9.047617e-02 1.758787e-01 22 33316085 33316248 164 - 2.065 2.037 -0.094
ENSG00000133424 E018 0.1472490 0.0430656686 3.141058e-01   22 33322485 33322582 98 - 0.145 0.000 -12.804
ENSG00000133424 E019 1.5510891 0.0087201522 7.447910e-01 8.329796e-01 22 33323211 33323600 390 - 0.340 0.416 0.433
ENSG00000133424 E020 116.4544632 0.0056777266 1.369183e-01 2.437922e-01 22 33337646 33337801 156 - 2.071 2.033 -0.128
ENSG00000133424 E021 103.6900981 0.0016183653 6.462387e-02 1.344155e-01 22 33381919 33382044 126 - 2.022 1.980 -0.140
ENSG00000133424 E022 103.1839907 0.0003067232 4.040406e-01 5.477543e-01 22 33384192 33384304 113 - 1.995 1.995 0.001
ENSG00000133424 E023 98.7775245 0.0003037977 8.123406e-02 1.614768e-01 22 33432161 33432265 105 - 2.000 1.967 -0.111
ENSG00000133424 E024 0.0000000       22 33437973 33438567 595 -      
ENSG00000133424 E025 0.0000000       22 33562912 33563263 352 -      
ENSG00000133424 E026 117.0815719 0.0003066543 1.005942e-03 3.979052e-03 22 33564848 33565019 172 - 2.098 2.019 -0.264
ENSG00000133424 E027 0.0000000       22 33572145 33572262 118 -      
ENSG00000133424 E028 0.6568746 0.0207508227 5.725663e-01 6.986658e-01 22 33604232 33604434 203 - 0.145 0.256 1.017
ENSG00000133424 E029 110.9192596 0.0028054894 1.555504e-02 4.174685e-02 22 33604435 33604558 124 - 2.070 2.002 -0.228
ENSG00000133424 E030 0.1515154 0.0434278534 3.136664e-01   22 33604559 33604707 149 - 0.145 0.000 -12.803
ENSG00000133424 E031 79.5885063 0.0044412394 1.939252e-02 5.018309e-02 22 33626244 33626326 83 - 1.939 1.854 -0.286
ENSG00000133424 E032 0.1472490 0.0430656686 3.141058e-01   22 33626327 33626328 2 - 0.145 0.000 -12.804
ENSG00000133424 E033 143.3971272 0.0053998642 1.418407e-02 3.864371e-02 22 33650367 33650668 302 - 2.186 2.111 -0.251
ENSG00000133424 E034 0.1817044 0.0398449864 7.378056e-01   22 33656962 33657216 255 - 0.000 0.103 11.256
ENSG00000133424 E035 91.6762977 0.0123311539 1.947406e-02 5.036041e-02 22 33761371 33761558 188 - 2.015 1.903 -0.376
ENSG00000133424 E036 8.0201325 0.0385661785 8.721286e-01 9.217620e-01 22 33856740 33856802 63 - 0.931 0.943 0.045
ENSG00000133424 E037 0.0000000       22 33861556 33861624 69 -      
ENSG00000133424 E038 0.1817044 0.0398449864 7.378056e-01   22 33873108 33873405 298 - 0.000 0.103 11.256
ENSG00000133424 E039 0.1817044 0.0398449864 7.378056e-01   22 33880552 33880672 121 - 0.000 0.103 11.256
ENSG00000133424 E040 0.0000000       22 33889138 33889228 91 -      
ENSG00000133424 E041 79.0719623 0.0117248362 4.447612e-02 9.924759e-02 22 33919995 33920476 482 - 1.939 1.846 -0.315
ENSG00000133424 E042 1.0319116 0.0121989077 7.902104e-01 8.658096e-01 22 33922477 33922620 144 - 0.253 0.316 0.433
ENSG00000133424 E043 0.0000000       22 33922621 33922841 221 -