ENSG00000133401

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000438447 ENSG00000133401 HEK293_OSMI2_2hA HEK293_TMG_2hB PDZD2 protein_coding protein_coding 1.158548 2.150139 0.5621621 0.2825483 0.01284627 -1.916628 0.09912048 0.05751862 0.1023130 0.01492979 0.01675217 0.7341678 0.151675000 0.02880000 0.1833333 0.15453333 0.01430311 0.01430311 FALSE TRUE
ENST00000513184 ENSG00000133401 HEK293_OSMI2_2hA HEK293_TMG_2hB PDZD2 protein_coding processed_transcript 1.158548 2.150139 0.5621621 0.2825483 0.01284627 -1.916628 0.01844657 0.14757257 0.0000000 0.14757257 0.00000000 -3.9779445 0.006795833 0.05436667 0.0000000 -0.05436667 1.00000000 0.01430311   FALSE
MSTRG.26008.2 ENSG00000133401 HEK293_OSMI2_2hA HEK293_TMG_2hB PDZD2 protein_coding   1.158548 2.150139 0.5621621 0.2825483 0.01284627 -1.916628 0.91396626 1.76220306 0.3741847 0.15098131 0.02938046 -2.2056719 0.714270833 0.82856667 0.6646333 -0.16393333 0.20579801 0.01430311 TRUE FALSE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000133401 E001 0.6568746 0.019655441 4.254450e-01 0.5683878403 5 31639131 31639137 7 + 0.143 0.258 1.052
ENSG00000133401 E002 6.4044524 0.021768165 9.239240e-04 0.0036988786 5 31639138 31639437 300 + 0.525 0.951 1.756
ENSG00000133401 E003 8.3302892 0.003602769 2.265258e-05 0.0001386669 5 31646141 31646255 115 + 0.616 1.073 1.789
ENSG00000133401 E004 6.6027348 0.023511708 3.103579e-03 0.0105708960 5 31646438 31646545 108 + 0.616 0.981 1.451
ENSG00000133401 E005 0.0000000       5 31692887 31692974 88 +      
ENSG00000133401 E006 0.0000000       5 31712039 31712091 53 +      
ENSG00000133401 E007 0.0000000       5 31787637 31787718 82 +      
ENSG00000133401 E008 0.8836184 0.016753718 8.368280e-01 0.8979583630 5 31798889 31799724 836 + 0.251 0.258 0.055
ENSG00000133401 E009 0.1472490 0.042931748 4.004581e-01   5 31843288 31843975 688 + 0.143 0.000 -13.245
ENSG00000133401 E010 0.1817044 0.039975639 5.888291e-01   5 31854937 31855168 232 + 0.000 0.104 11.376
ENSG00000133401 E011 0.0000000       5 31936152 31936346 195 +      
ENSG00000133401 E012 1.2586568 0.011497636 3.073881e-01 0.4496189457 5 31983155 31983656 502 + 0.471 0.258 -1.270
ENSG00000133401 E013 0.2934659 0.030602064 8.695802e-01   5 31995576 31995718 143 + 0.143 0.104 -0.535
ENSG00000133401 E014 0.7300161 0.032578500 9.701639e-03 0.0280268745 5 32000139 32000271 133 + 0.471 0.000 -14.932
ENSG00000133401 E015 0.6653823 0.017577549 2.202963e-01 0.3507775787 5 32010330 32010482 153 + 0.337 0.104 -2.116
ENSG00000133401 E016 0.4439371 0.022005124 5.503793e-02 0.1180607478 5 32037231 32037342 112 + 0.337 0.000 -14.455
ENSG00000133401 E017 0.6213751 0.019857913 2.216650e-01 0.3523853710 5 32048539 32048684 146 + 0.337 0.104 -2.117
ENSG00000133401 E018 0.0000000       5 32052494 32052610 117 +      
ENSG00000133401 E019 1.0589695 0.015070101 2.482647e-02 0.0615188445 5 32052611 32052730 120 + 0.525 0.104 -3.117
ENSG00000133401 E020 0.5848434 0.019968202 2.187984e-02 0.0554267853 5 32053769 32053883 115 + 0.409 0.000 -14.734
ENSG00000133401 E021 0.4355181 0.671269033 2.342908e-01 0.3675103457 5 32057655 32057728 74 + 0.336 0.000 -14.156
ENSG00000133401 E022 0.5891098 0.019004213 2.159438e-02 0.0548390144 5 32057878 32058103 226 + 0.409 0.000 -14.735
ENSG00000133401 E023 0.9588482 0.013578084 2.773194e-01 0.4168736463 5 32059239 32059331 93 + 0.409 0.188 -1.532
ENSG00000133401 E024 0.9588482 0.013578084 2.773194e-01 0.4168736463 5 32059332 32059356 25 + 0.409 0.188 -1.532
ENSG00000133401 E025 1.2920680 0.010735764 3.084674e-01 0.4507591751 5 32061002 32061134 133 + 0.471 0.258 -1.269
ENSG00000133401 E026 0.9159869 0.015495488 5.126809e-02 0.1114135204 5 32069569 32069650 82 + 0.471 0.104 -2.854
ENSG00000133401 E027 0.4783925 0.023236186 4.459573e-01 0.5875110441 5 32071384 32071418 35 + 0.251 0.104 -1.532
ENSG00000133401 E028 0.7395785 0.189590907 8.441766e-01 0.9029847592 5 32072161 32072317 157 + 0.251 0.192 -0.497
ENSG00000133401 E029 1.0414660 0.013129158 1.480074e-03 0.0055728017 5 32073832 32074643 812 + 0.573 0.000 -15.236
ENSG00000133401 E030 0.0000000       5 32077460 32077461 2 +      
ENSG00000133401 E031 0.2955422 0.028789528 8.708274e-01   5 32077462 32077606 145 + 0.143 0.104 -0.535
ENSG00000133401 E032 0.0000000       5 32077607 32077955 349 +      
ENSG00000133401 E033 0.1482932 0.041916838 5.912561e-01   5 32083414 32083615 202 + 0.000 0.104 11.372
ENSG00000133401 E034 7.0200672 0.002630172 4.417747e-05 0.0002522086 5 32087131 32091175 4045 + 1.144 0.628 -1.992
ENSG00000133401 E035 0.9942340 0.014053325 5.782550e-01 0.7033939010 5 32092907 32093024 118 + 0.251 0.318 0.467
ENSG00000133401 E036 0.6986918 0.017148655 4.225285e-01 0.5656575503 5 32097279 32097380 102 + 0.143 0.258 1.053
ENSG00000133401 E037 2.0896585 0.010684776 1.111055e-01 0.2071652482 5 32098364 32098596 233 + 0.337 0.568 1.201
ENSG00000133401 E038 1.3168578 0.475810664 7.537620e-01 0.8395830023 5 32098597 32098634 38 + 0.337 0.370 0.199
ENSG00000133401 E039 0.1515154 0.044190843 3.987439e-01   5 32099174 32100861 1688 + 0.143 0.000 -13.245
ENSG00000133401 E040 0.0000000       5 32100862 32100866 5 +      
ENSG00000133401 E041 0.1515154 0.044190843 3.987439e-01   5 32100867 32100963 97 + 0.143 0.000 -13.245
ENSG00000133401 E042 1.7952503 0.196595384 8.842869e-01 0.9300629232 5 32101105 32101239 135 + 0.471 0.419 -0.269
ENSG00000133401 E043 0.0000000       5 32107321 32107460 140 +      
ENSG00000133401 E044 16.3038948 0.003615506 3.744063e-01 0.5188213941 5 32107969 32110932 2964 + 1.222 1.229 0.026