ENSG00000133398

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000255764 ENSG00000133398 HEK293_OSMI2_2hA HEK293_TMG_2hB MED10 protein_coding protein_coding 25.66179 30.31036 27.25276 3.414104 1.002718 -0.1533553 22.809582 29.1386894 23.656865 3.2417416 0.71039190 -0.3005624 0.8859042 0.96180000 0.86853333 -0.09326667 2.593946e-05 2.593946e-05 FALSE  
ENST00000503112 ENSG00000133398 HEK293_OSMI2_2hA HEK293_TMG_2hB MED10 protein_coding retained_intron 25.66179 30.31036 27.25276 3.414104 1.002718 -0.1533553 1.663141 0.8613918 2.148584 0.3957744 0.05777915 1.3086921 0.0670875 0.02763333 0.07913333 0.05150000 1.955359e-01 2.593946e-05    
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000133398 E001 103.744952 0.0005983419 2.894545e-11 5.590406e-10 5 6371874 6372164 291 - 1.850 2.095 0.822
ENSG00000133398 E002 478.421098 0.0001509185 5.999043e-03 1.860798e-02 5 6372165 6372601 437 - 2.651 2.686 0.117
ENSG00000133398 E003 228.086425 0.0003692141 3.760454e-01 5.204190e-01 5 6374324 6374426 103 - 2.364 2.342 -0.074
ENSG00000133398 E004 15.254985 0.0223041375 2.819588e-06 2.123822e-05 5 6374427 6374811 385 - 1.446 0.930 -1.841
ENSG00000133398 E005 7.397424 0.0023266045 3.099496e-07 2.859042e-06 5 6376812 6377165 354 - 1.188 0.579 -2.368
ENSG00000133398 E006 200.827624 0.0002435971 7.464772e-02 1.509762e-01 5 6377166 6377249 84 - 2.324 2.281 -0.146
ENSG00000133398 E007 211.344457 0.0002301549 3.678639e-03 1.223453e-02 5 6378362 6378571 210 - 2.360 2.293 -0.226