ENSG00000133302

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000265140 ENSG00000133302 HEK293_OSMI2_2hA HEK293_TMG_2hB SLF1 protein_coding protein_coding 6.962772 2.198509 12.42462 0.54991 1.378133 2.493218 3.7428222 0.7547532 6.376627 0.09455919 0.2219852 3.061988 0.48351667 0.3919667 0.5237333 0.13176667 6.000578e-01 3.143028e-06 FALSE TRUE
ENST00000508130 ENSG00000133302 HEK293_OSMI2_2hA HEK293_TMG_2hB SLF1 protein_coding nonsense_mediated_decay 6.962772 2.198509 12.42462 0.54991 1.378133 2.493218 0.7533202 0.0000000 1.749617 0.00000000 0.3270455 7.459117 0.06457083 0.0000000 0.1425000 0.14250000 3.143028e-06 3.143028e-06 TRUE TRUE
MSTRG.26604.12 ENSG00000133302 HEK293_OSMI2_2hA HEK293_TMG_2hB SLF1 protein_coding   6.962772 2.198509 12.42462 0.54991 1.378133 2.493218 0.5560061 0.2649492 1.248359 0.15717212 0.2039065 2.194307 0.07321667 0.1690667 0.1010000 -0.06806667 1.000000e+00 3.143028e-06   FALSE
MSTRG.26604.13 ENSG00000133302 HEK293_OSMI2_2hA HEK293_TMG_2hB SLF1 protein_coding   6.962772 2.198509 12.42462 0.54991 1.378133 2.493218 1.2821452 1.0046933 2.512534 0.66390556 0.8707644 1.313830 0.29432500 0.3652667 0.1912667 -0.17400000 8.824521e-01 3.143028e-06   FALSE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000133302 E001 1.0694649 0.4139245053 7.911178e-01 0.8664190181 5 94618669 94618670 2 + 0.237 0.274 0.274
ENSG00000133302 E002 1.8058238 0.1217031214 6.936079e-01 0.7946320337 5 94618671 94618677 7 + 0.368 0.273 -0.604
ENSG00000133302 E003 4.8837464 0.0930144280 9.749925e-02 0.1866675516 5 94618678 94618684 7 + 0.687 0.273 -2.141
ENSG00000133302 E004 5.3287277 0.0540805870 2.060331e-01 0.3337210445 5 94618685 94618686 2 + 0.708 0.439 -1.235
ENSG00000133302 E005 11.5543431 0.0099273506 1.838393e-03 0.0067216980 5 94618687 94618708 22 + 1.009 0.439 -2.395
ENSG00000133302 E006 18.0129057 0.0010506085 2.734963e-04 0.0012718291 5 94618709 94618765 57 + 1.181 0.653 -2.018
ENSG00000133302 E007 0.1451727 0.0427976032 8.024535e-01   5 94620065 94620171 107 + 0.050 0.001 -6.584
ENSG00000133302 E008 26.2737365 0.0015675661 4.931129e-02 0.1079406597 5 94628811 94628881 71 + 1.309 1.122 -0.661
ENSG00000133302 E009 20.6678833 0.0038503655 9.867669e-01 0.9957813441 5 94628882 94628924 43 + 1.181 1.201 0.073
ENSG00000133302 E010 25.3009416 0.0017789621 3.386877e-01 0.4825583467 5 94629092 94629167 76 + 1.277 1.201 -0.268
ENSG00000133302 E011 0.0000000       5 94629664 94629720 57 +      
ENSG00000133302 E012 47.6290582 0.0004623689 3.055267e-02 0.0730527116 5 94630503 94630743 241 + 1.552 1.406 -0.499
ENSG00000133302 E013 39.9006795 0.0005562309 1.206527e-02 0.0336899747 5 94643273 94643435 163 + 1.483 1.287 -0.680
ENSG00000133302 E014 38.4351503 0.0006069983 1.077817e-02 0.0306346674 5 94649454 94649597 144 + 1.471 1.267 -0.708
ENSG00000133302 E015 38.6020288 0.0005338076 1.083398e-02 0.0307674456 5 94651702 94651845 144 + 1.471 1.267 -0.708
ENSG00000133302 E016 41.0043413 0.0025790997 2.709308e-03 0.0094015885 5 94653272 94653421 150 + 1.500 1.246 -0.881
ENSG00000133302 E017 35.7765950 0.0024142380 6.968430e-02 0.1428407050 5 94654630 94654752 123 + 1.432 1.287 -0.501
ENSG00000133302 E018 27.8945424 0.0007381370 9.634945e-01 0.9809862713 5 94662298 94662351 54 + 1.306 1.325 0.064
ENSG00000133302 E019 45.1184386 0.0005647497 6.778261e-01 0.7824377922 5 94663750 94663908 159 + 1.503 1.556 0.181
ENSG00000133302 E020 26.3024663 0.0007376487 4.566799e-01 0.5970700017 5 94665861 94665906 46 + 1.296 1.246 -0.176
ENSG00000133302 E021 41.5312446 0.0041089760 6.643932e-01 0.7722194977 5 94665907 94666024 118 + 1.467 1.524 0.195
ENSG00000133302 E022 33.8164108 0.0009926685 1.895322e-01 0.3131655017 5 94670151 94670279 129 + 1.402 1.306 -0.331
ENSG00000133302 E023 44.9850111 0.0005169691 4.200963e-01 0.5633208894 5 94670843 94671008 166 + 1.513 1.475 -0.130
ENSG00000133302 E024 0.8147077 0.1644826547 6.418117e-01 0.7547629998 5 94678399 94678807 409 + 0.172 0.275 0.868
ENSG00000133302 E025 47.0103158 0.0004490994 4.231013e-01 0.5662162506 5 94678808 94678955 148 + 1.518 1.596 0.268
ENSG00000133302 E026 46.7779087 0.0005627539 6.466585e-01 0.7585038474 5 94686573 94686718 146 + 1.521 1.577 0.190
ENSG00000133302 E027 46.2195225 0.0006029249 7.399515e-01 0.8295014620 5 94688506 94688669 164 + 1.520 1.566 0.160
ENSG00000133302 E028 38.3969089 0.0005469331 8.920113e-01 0.9350398405 5 94689473 94689606 134 + 1.442 1.475 0.116
ENSG00000133302 E029 0.3030308 0.3682568187 1.000000e+00   5 94691195 94691563 369 + 0.095 0.001 -7.591
ENSG00000133302 E030 37.4126185 0.0005349888 1.787855e-02 0.0468768068 5 94691564 94691656 93 + 1.402 1.596 0.668
ENSG00000133302 E031 48.5228078 0.0006052120 1.268688e-01 0.2297881308 5 94692074 94692185 112 + 1.526 1.651 0.425
ENSG00000133302 E032 41.6607351 0.0005257473 1.535506e-01 0.2665014646 5 94692186 94692256 71 + 1.462 1.587 0.427
ENSG00000133302 E033 170.4907541 0.0098920914 7.784596e-05 0.0004178879 5 94694831 94697621 2791 + 2.042 2.267 0.754
ENSG00000133302 E034 2.1100125 0.0069638502 8.667129e-01 0.9182203648 5 94697831 94697917 87 + 0.390 0.439 0.267
ENSG00000133302 E035 2.3574835 0.0093890620 3.998782e-01 0.5436012755 5 94701695 94701771 77 + 0.450 0.273 -1.055
ENSG00000133302 E036 1.1050531 0.0119235353 9.147398e-01 0.9500233274 5 94702010 94702027 18 + 0.237 0.273 0.269
ENSG00000133302 E037 2.4757000 0.0133561308 9.825227e-01 0.9931224606 5 94702523 94702576 54 + 0.431 0.440 0.047
ENSG00000133302 E038 5.4348479 0.0034842573 2.055377e-01 0.3330895730 5 94708225 94708289 65 + 0.630 0.853 0.907
ENSG00000133302 E039 8.8754598 0.0023426711 6.118415e-04 0.0025765188 5 94714588 94714730 143 + 0.758 1.201 1.655
ENSG00000133302 E040 0.0000000       5 94739164 94739436 273 +