ENSG00000133275

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000255641 ENSG00000133275 HEK293_OSMI2_2hA HEK293_TMG_2hB CSNK1G2 protein_coding protein_coding 92.20734 144.0944 60.20498 12.18136 0.07701862 -1.25892 62.665331 106.386621 36.116326 9.6120980 1.1636739 -1.5583299 0.65313333 0.73780000 0.59993333 -0.13786667 3.260007e-03 3.019576e-15 FALSE TRUE
ENST00000585959 ENSG00000133275 HEK293_OSMI2_2hA HEK293_TMG_2hB CSNK1G2 protein_coding protein_coding 92.20734 144.0944 60.20498 12.18136 0.07701862 -1.25892 13.024952 14.154617 11.843025 1.4833894 1.9213066 -0.2570363 0.16104167 0.09796667 0.19660000 0.09863333 1.262030e-02 3.019576e-15 FALSE FALSE
ENST00000590106 ENSG00000133275 HEK293_OSMI2_2hA HEK293_TMG_2hB CSNK1G2 protein_coding retained_intron 92.20734 144.0944 60.20498 12.18136 0.07701862 -1.25892 5.185989 6.287736 5.031768 0.9288668 0.9586015 -0.3209033 0.06106250 0.04333333 0.08353333 0.04020000 1.196233e-01 3.019576e-15 TRUE FALSE
ENST00000615564 ENSG00000133275 HEK293_OSMI2_2hA HEK293_TMG_2hB CSNK1G2 protein_coding nonsense_mediated_decay 92.20734 144.0944 60.20498 12.18136 0.07701862 -1.25892 3.142388 7.786837 0.000000 1.5890666 0.0000000 -9.6067452 0.02568333 0.05620000 0.00000000 -0.05620000 3.019576e-15 3.019576e-15 TRUE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000133275 E001 5.2229823 0.0146332414 3.473362e-13 9.145490e-12 19 1941144 1941171 28 + 1.252 0.244 -4.479
ENSG00000133275 E002 10.7034489 0.0175689017 1.845008e-19 1.109975e-17 19 1941172 1941190 19 + 1.533 0.462 -4.125
ENSG00000133275 E003 11.5044520 0.0264182920 6.678450e-17 2.937572e-15 19 1941191 1941194 4 + 1.554 0.516 -3.929
ENSG00000133275 E004 175.2338346 0.0084094657 9.709626e-17 4.180590e-15 19 1941195 1941418 224 + 2.448 2.026 -1.408
ENSG00000133275 E005 0.9212829 0.0150613118 4.656824e-01 6.053392e-01 19 1942459 1942613 155 + 0.327 0.195 -0.985
ENSG00000133275 E006 0.1515154 0.0422165460 1.544949e-01   19 1954621 1954632 12 + 0.194 0.000 -10.428
ENSG00000133275 E007 0.3332198 0.0274424043 4.220738e-01   19 1954633 1954715 83 + 0.194 0.075 -1.573
ENSG00000133275 E008 0.5546650 0.0208667384 7.342040e-01 8.252216e-01 19 1959238 1959303 66 + 0.194 0.139 -0.571
ENSG00000133275 E009 8.5501720 0.0021692517 8.929759e-05 4.716900e-04 19 1968791 1968905 115 + 1.193 0.757 -1.629
ENSG00000133275 E010 840.0462495 0.0005413640 4.646993e-15 1.606494e-13 19 1969508 1969825 318 + 2.933 2.806 -0.422
ENSG00000133275 E011 637.5817469 0.0001526722 8.569142e-02 1.684984e-01 19 1969826 1969959 134 + 2.733 2.709 -0.080
ENSG00000133275 E012 2.2434381 0.0066347302 6.404928e-01 7.537172e-01 19 1974688 1974751 64 + 0.511 0.431 -0.403
ENSG00000133275 E013 460.2423820 0.0002049703 6.753029e-01 7.806220e-01 19 1978305 1978345 41 + 2.563 2.577 0.046
ENSG00000133275 E014 10.5060799 0.0016754474 1.219912e-01 2.227704e-01 19 1978346 1978441 96 + 1.107 0.941 -0.608
ENSG00000133275 E015 387.1100304 0.0001308453 6.478002e-01 7.594248e-01 19 1978442 1978452 11 + 2.487 2.502 0.049
ENSG00000133275 E016 422.4713372 0.0001415089 3.647871e-01 5.091153e-01 19 1978453 1978474 22 + 2.517 2.541 0.080
ENSG00000133275 E017 458.7661933 0.0011897240 6.611329e-01 7.696541e-01 19 1978475 1978511 37 + 2.558 2.578 0.068
ENSG00000133275 E018 52.3094822 0.0047457816 1.078558e-01 2.023605e-01 19 1978512 1978601 90 + 1.707 1.613 -0.321
ENSG00000133275 E019 288.1777002 0.0002407423 5.302800e-01 6.629139e-01 19 1978602 1978603 2 + 2.378 2.368 -0.036
ENSG00000133275 E020 440.9813445 0.0001452287 2.695148e-01 4.080146e-01 19 1978604 1978642 39 + 2.569 2.551 -0.058
ENSG00000133275 E021 763.5989636 0.0002334284 9.604045e-02 1.844244e-01 19 1978643 1978750 108 + 2.811 2.790 -0.070
ENSG00000133275 E022 802.2328801 0.0001686150 2.991064e-04 1.375961e-03 19 1978859 1978933 75 + 2.854 2.804 -0.166
ENSG00000133275 E023 1155.4469075 0.0011790057 4.820865e-04 2.091651e-03 19 1978934 1979093 160 + 3.023 2.964 -0.197
ENSG00000133275 E024 37.5143040 0.0198627071 2.153528e-03 7.700267e-03 19 1979094 1979162 69 + 1.693 1.425 -0.916
ENSG00000133275 E025 621.9046325 0.0010155623 3.067016e-03 1.046442e-02 19 1979163 1979203 41 + 2.750 2.694 -0.189
ENSG00000133275 E026 564.2119473 0.0001269081 2.606984e-01 3.982671e-01 19 1979204 1979248 45 + 2.675 2.660 -0.050
ENSG00000133275 E027 2.8000792 0.0218413583 6.840785e-01 7.871324e-01 19 1979258 1979318 61 + 0.580 0.513 -0.315
ENSG00000133275 E028 513.9511163 0.0002353773 4.313913e-01 5.739493e-01 19 1979319 1979333 15 + 2.632 2.622 -0.033
ENSG00000133275 E029 764.6810592 0.0001177622 8.984061e-01 9.392085e-01 19 1979334 1979403 70 + 2.790 2.797 0.024
ENSG00000133275 E030 1017.7033212 0.0001113106 9.401240e-01 9.663853e-01 19 1979495 1979618 124 + 2.913 2.920 0.022
ENSG00000133275 E031 592.8199364 0.0007492753 8.378141e-01 8.986028e-01 19 1979619 1979643 25 + 2.675 2.688 0.042
ENSG00000133275 E032 900.4681224 0.0002550850 8.480188e-01 9.056439e-01 19 1979752 1979835 84 + 2.858 2.868 0.032
ENSG00000133275 E033 16.1521748 0.0192050676 1.039534e-01 1.965258e-01 19 1979836 1979910 75 + 1.291 1.119 -0.610
ENSG00000133275 E034 924.9867419 0.0001240760 1.938400e-02 5.016790e-02 19 1979911 1980017 107 + 2.847 2.886 0.131
ENSG00000133275 E035 1595.6733856 0.0006567277 1.157838e-16 4.937906e-15 19 1980149 1980579 431 + 3.004 3.144 0.467
ENSG00000133275 E036 1954.1215186 0.0011294769 4.183374e-20 2.690182e-18 19 1980580 1981481 902 + 3.064 3.239 0.582
ENSG00000133275 E037 2.2604812 0.0070089263 3.252996e-04 1.482132e-03 19 1981714 1981776 63 + 0.821 0.244 -2.894