ENSG00000133216

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000374630 ENSG00000133216 HEK293_OSMI2_2hA HEK293_TMG_2hB EPHB2 protein_coding protein_coding 8.060385 8.921117 8.81657 1.387299 0.8514522 -0.01698757 1.6257579 1.274888 2.2006916 0.3291673 0.2419177 0.7828550 0.20586250 0.1622333 0.25406667 0.09183333 0.477876764 0.000495171 FALSE TRUE
ENST00000374632 ENSG00000133216 HEK293_OSMI2_2hA HEK293_TMG_2hB EPHB2 protein_coding protein_coding 8.060385 8.921117 8.81657 1.387299 0.8514522 -0.01698757 0.5754384 0.875261 0.6026517 0.2640459 0.1962021 -0.5310356 0.07230417 0.1037000 0.07276667 -0.03093333 0.760934070 0.000495171 FALSE TRUE
ENST00000465676 ENSG00000133216 HEK293_OSMI2_2hA HEK293_TMG_2hB EPHB2 protein_coding processed_transcript 8.060385 8.921117 8.81657 1.387299 0.8514522 -0.01698757 2.8751129 2.328604 3.0543294 0.4527136 0.4927021 0.3899236 0.35859167 0.2617000 0.34233333 0.08063333 0.348016191 0.000495171 FALSE TRUE
ENST00000544305 ENSG00000133216 HEK293_OSMI2_2hA HEK293_TMG_2hB EPHB2 protein_coding protein_coding 8.060385 8.921117 8.81657 1.387299 0.8514522 -0.01698757 1.7022477 1.694470 2.3895978 0.9096123 0.3545552 0.4934697 0.20218333 0.1645333 0.26866667 0.10413333 0.712612085 0.000495171 FALSE TRUE
MSTRG.516.6 ENSG00000133216 HEK293_OSMI2_2hA HEK293_TMG_2hB EPHB2 protein_coding   8.060385 8.921117 8.81657 1.387299 0.8514522 -0.01698757 1.1392080 2.565898 0.5159073 0.4021132 0.2561017 -2.2921949 0.14067500 0.2879667 0.05526667 -0.23270000 0.000495171 0.000495171 FALSE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000133216 E001 5.8366638 0.0027678308 0.0002675154 0.001247405 1 22710839 22710964 126 + 1.047 0.601 -1.763
ENSG00000133216 E002 4.5238805 0.0515439586 0.0818768889 0.162506929 1 22710965 22710969 5 + 0.883 0.601 -1.151
ENSG00000133216 E003 12.7994019 0.0293045733 0.0354035011 0.082430065 1 22710970 22711043 74 + 1.260 1.013 -0.887
ENSG00000133216 E004 0.3697384 0.0247134716 0.3297507381 0.473288442 1 22775178 22775244 67 + 0.000 0.188 11.227
ENSG00000133216 E005 2.7698888 0.0054896951 0.2073959680 0.335380906 1 22780968 22781040 73 + 0.408 0.629 1.063
ENSG00000133216 E006 3.3870226 0.0048562096 0.1596098114 0.274601253 1 22781041 22781105 65 + 0.470 0.705 1.063
ENSG00000133216 E007 2.4595859 0.0261067772 0.7402915696 0.829753368 1 22781106 22781137 32 + 0.470 0.539 0.334
ENSG00000133216 E008 28.9148653 0.0062472413 0.3922271905 0.536166656 1 22781421 22781485 65 + 1.486 1.438 -0.166
ENSG00000133216 E009 179.7777199 0.0051079222 0.1890247946 0.312517936 1 22784392 22785076 685 + 2.259 2.225 -0.112
ENSG00000133216 E010 0.0000000       1 22800240 22800291 52 +      
ENSG00000133216 E011 80.1653422 0.0034944851 0.2194847684 0.349775019 1 22863037 22863192 156 + 1.914 1.872 -0.141
ENSG00000133216 E012 119.9588616 0.0059972711 0.0468947596 0.103640592 1 22864877 22865158 282 + 2.113 2.034 -0.264
ENSG00000133216 E013 55.8033599 0.0004528023 0.0191408796 0.049645166 1 22865159 22865212 54 + 1.791 1.696 -0.322
ENSG00000133216 E014 70.4352930 0.0003661400 0.0081969707 0.024283524 1 22882359 22882483 125 + 1.891 1.795 -0.323
ENSG00000133216 E015 0.7739347 0.0148635809 0.3771187748 0.521458751 1 22882484 22882840 357 + 0.336 0.188 -1.108
ENSG00000133216 E016 0.2214452 0.0378167441 0.6944135254   1 22891129 22891265 137 + 0.000 0.105 10.230
ENSG00000133216 E017 0.2966881 0.0295354232 0.1132695984   1 22892881 22892883 3 + 0.250 0.000 -12.545
ENSG00000133216 E018 74.9791507 0.0011046427 0.1312952458 0.235946599 1 22892884 22893046 163 + 1.895 1.847 -0.164
ENSG00000133216 E019 69.5503415 0.0003986695 0.0011516355 0.004476831 1 22895472 22895580 109 + 1.902 1.780 -0.411
ENSG00000133216 E020 4.6343157 0.0303112967 0.4620089893 0.601924474 1 22896411 22896413 3 + 0.783 0.685 -0.399
ENSG00000133216 E021 71.2892404 0.0003996681 0.0009901549 0.003924800 1 22896414 22896478 65 + 1.910 1.788 -0.413
ENSG00000133216 E022 96.3395177 0.0002875481 0.0014606563 0.005509508 1 22905987 22906109 123 + 2.028 1.929 -0.332
ENSG00000133216 E023 157.1938450 0.0019528895 0.1041678531 0.196848782 1 22906710 22906957 248 + 2.202 2.164 -0.127
ENSG00000133216 E024 149.5815245 0.0022464635 0.9408378329 0.966824448 1 22907953 22908168 216 + 2.147 2.163 0.052
ENSG00000133216 E025 114.6547184 0.0007191683 0.0103415160 0.029608118 1 22909022 22909171 150 + 1.974 2.082 0.363
ENSG00000133216 E026 133.4410885 0.0004179639 0.0007562987 0.003102005 1 22910382 22910575 194 + 2.026 2.152 0.422
ENSG00000133216 E027 121.6788402 0.0004410507 0.0705689483 0.144287208 1 22912444 22912599 156 + 2.018 2.096 0.259
ENSG00000133216 E028 0.1482932 0.0415993390 0.6960389525   1 22912600 22913461 862 + 0.000 0.104 10.223
ENSG00000133216 E029 94.1725111 0.0003195857 0.0110377428 0.031251005 1 22913462 22913564 103 + 1.884 1.997 0.379
ENSG00000133216 E030 83.6225011 0.0054640074 0.1551355483 0.268573190 1 22913565 22913657 93 + 1.848 1.940 0.312
ENSG00000133216 E031 608.5567945 0.0063463669 0.0104208509 0.029799229 1 22913658 22921500 7843 + 2.699 2.804 0.347