ENSG00000133193

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000403627 ENSG00000133193 HEK293_OSMI2_2hA HEK293_TMG_2hB FAM104A protein_coding protein_coding 14.99924 18.38399 15.73994 1.076354 0.08376396 -0.2238879 10.3141350 11.578035 10.954422 0.5629552 0.46151546 -0.07980622 0.68550000 0.63603333 0.69573333 0.0597000 0.7316294236 0.0002283948 FALSE TRUE
ENST00000405159 ENSG00000133193 HEK293_OSMI2_2hA HEK293_TMG_2hB FAM104A protein_coding protein_coding 14.99924 18.38399 15.73994 1.076354 0.08376396 -0.2238879 1.9024995 1.209555 3.367728 0.2260853 0.24773073 1.46969880 0.12560000 0.06726667 0.21413333 0.1468667 0.0002283948 0.0002283948 FALSE TRUE
ENST00000580032 ENSG00000133193 HEK293_OSMI2_2hA HEK293_TMG_2hB FAM104A protein_coding processed_transcript 14.99924 18.38399 15.73994 1.076354 0.08376396 -0.2238879 0.6534592 2.279239 0.000000 1.2696958 0.00000000 -7.83872444 0.04467917 0.11843333 0.00000000 -0.1184333 0.1897961366 0.0002283948 FALSE FALSE
ENST00000581110 ENSG00000133193 HEK293_OSMI2_2hA HEK293_TMG_2hB FAM104A protein_coding protein_coding 14.99924 18.38399 15.73994 1.076354 0.08376396 -0.2238879 1.9185044 3.232820 1.208297 0.6202921 0.09734641 -1.41238315 0.12890417 0.17343333 0.07673333 -0.0967000 0.0023286789 0.0002283948   FALSE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000133193 E001 2.3229224 0.0063235735 3.348091e-01 4.785444e-01 17 73207353 73207364 12 - 0.609 0.425 -0.881
ENSG00000133193 E002 739.9882173 0.0016445552 1.731766e-20 1.172125e-18 17 73207365 73209487 2123 - 2.960 2.783 -0.589
ENSG00000133193 E003 80.6274611 0.0003780059 4.206960e-02 9.485450e-02 17 73209488 73209507 20 - 1.874 1.918 0.149
ENSG00000133193 E004 125.6725640 0.0003223304 1.329737e-02 3.661232e-02 17 73209508 73209565 58 - 2.065 2.107 0.140
ENSG00000133193 E005 93.7932219 0.0003921697 7.589182e-02 1.529324e-01 17 73209566 73209574 9 - 1.946 1.974 0.096
ENSG00000133193 E006 108.7844852 0.0014344097 2.748328e-03 9.516770e-03 17 73209575 73209604 30 - 1.978 2.053 0.251
ENSG00000133193 E007 195.0781114 0.0002440097 2.228752e-07 2.117398e-06 17 73209605 73209768 164 - 2.218 2.313 0.317
ENSG00000133193 E008 19.7862717 0.0019058189 3.306553e-01 4.742598e-01 17 73212678 73212740 63 - 1.365 1.253 -0.393
ENSG00000133193 E009 77.7356500 0.0004576307 5.597205e-03 1.754685e-02 17 73227165 73227182 18 - 1.835 1.911 0.258
ENSG00000133193 E010 131.8590471 0.0025447417 5.942205e-04 2.512236e-03 17 73227183 73227264 82 - 2.050 2.143 0.309
ENSG00000133193 E011 1.2899917 0.0115726156 3.034978e-01 4.454184e-01 17 73227636 73227721 86 - 0.464 0.263 -1.204
ENSG00000133193 E012 0.3666179 0.0297795198 8.658513e-01 9.176101e-01 17 73227722 73227748 27 - 0.141 0.106 -0.464
ENSG00000133193 E013 154.4797355 0.0011633889 2.477314e-06 1.889142e-05 17 73232086 73232394 309 - 2.103 2.216 0.378
ENSG00000133193 E014 0.1515154 0.0429318439 3.957864e-01   17 73236572 73236753 182 - 0.141 0.000 -9.285