ENSG00000133124

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000372129 ENSG00000133124 HEK293_OSMI2_2hA HEK293_TMG_2hB IRS4 protein_coding protein_coding 117.4904 92.3548 134.9431 2.831611 2.312855 0.5470432 75.442766 77.316574 67.61965 0.9197723 1.359901 -0.1933084 0.6552167 0.83836667 0.5012000 -0.33716667 3.459808e-12 4.374981e-19 FALSE TRUE
ENST00000564206 ENSG00000133124 HEK293_OSMI2_2hA HEK293_TMG_2hB IRS4 protein_coding protein_coding 117.4904 92.3548 134.9431 2.831611 2.312855 0.5470432 15.545697 3.350621 24.63607 0.9299772 2.030812 2.8745582 0.1263542 0.03576667 0.1822667 0.14650000 2.054888e-05 4.374981e-19 FALSE TRUE
MSTRG.34716.3 ENSG00000133124 HEK293_OSMI2_2hA HEK293_TMG_2hB IRS4 protein_coding   117.4904 92.3548 134.9431 2.831611 2.312855 0.5470432 14.674576 4.407081 25.39189 2.2131118 0.869957 2.5237708 0.1189125 0.04783333 0.1884333 0.14060000 4.347541e-01 4.374981e-19 FALSE TRUE
MSTRG.34716.5 ENSG00000133124 HEK293_OSMI2_2hA HEK293_TMG_2hB IRS4 protein_coding   117.4904 92.3548 134.9431 2.831611 2.312855 0.5470432 1.546867 5.107343 0.00000 2.4797383 0.000000 -8.9992511 0.0176750 0.05483333 0.0000000 -0.05483333 7.882664e-10 4.374981e-19 FALSE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000133124 E001 5.718055 0.044492592 1.094573e-08 1.339644e-07 X 108719946 108719948 3 - 0.110 1.103 5.343
ENSG00000133124 E002 284.770330 0.007157064 8.325046e-54 6.411199e-51 X 108719949 108720047 99 - 1.945 2.696 2.509
ENSG00000133124 E003 846.760188 0.009325552 8.021459e-29 1.219616e-26 X 108720048 108720156 109 - 2.591 3.125 1.777
ENSG00000133124 E004 1586.223622 0.011770536 2.441818e-18 1.282137e-16 X 108720157 108720243 87 - 2.928 3.375 1.484
ENSG00000133124 E005 1713.132393 0.008981525 2.295775e-23 2.110275e-21 X 108720244 108720372 129 - 2.965 3.407 1.471
ENSG00000133124 E006 5696.994269 0.004116020 2.608528e-27 3.466150e-25 X 108720373 108721282 910 - 3.607 3.872 0.881
ENSG00000133124 E007 5148.762887 0.002152007 5.512405e-06 3.886233e-05 X 108721283 108722521 1239 - 3.677 3.745 0.227
ENSG00000133124 E008 417.016429 0.002337417 5.238722e-02 1.134124e-01 X 108722522 108722523 2 - 2.656 2.578 -0.262
ENSG00000133124 E009 243.181312 0.031834547 6.479634e-05 3.548850e-04 X 108722524 108723001 478 - 2.530 2.153 -1.260
ENSG00000133124 E010 193.464922 0.009263471 1.657685e-05 1.047405e-04 X 108723002 108723027 26 - 2.392 2.137 -0.852
ENSG00000133124 E011 205.919018 0.024972136 1.171888e-05 7.665054e-05 X 108723028 108723137 110 - 2.458 2.080 -1.262
ENSG00000133124 E012 2459.506611 0.070360933 1.718169e-07 1.668129e-06 X 108723138 108728525 5388 - 3.596 2.945 -2.167
ENSG00000133124 E013 132.680083 0.009710705 6.204576e-21 4.437291e-19 X 108728526 108728635 110 - 2.320 1.717 -2.024
ENSG00000133124 E014 790.635347 0.047049466 1.855393e-20 1.251330e-18 X 108728636 108732578 3943 - 3.152 2.104 -3.492
ENSG00000133124 E015 4725.771559 0.005592299 8.454303e-07 7.134264e-06 X 108732579 108734362 1784 - 3.751 3.574 -0.588
ENSG00000133124 E016 1238.781599 0.004738208 1.931610e-13 5.277442e-12 X 108734363 108734720 358 - 3.205 2.930 -0.916
ENSG00000133124 E017 768.761801 0.006641627 7.311325e-12 1.555952e-10 X 108734721 108734837 117 - 3.009 2.701 -1.025
ENSG00000133124 E018 528.717923 0.009740767 6.028112e-09 7.755570e-08 X 108734838 108734891 54 - 2.849 2.532 -1.056
ENSG00000133124 E019 2207.199948 0.013020829 5.356814e-09 6.950907e-08 X 108734892 108736563 1672 - 3.474 3.143 -1.099