ENSG00000132970

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000335327 ENSG00000132970 HEK293_OSMI2_2hA HEK293_TMG_2hB WASF3 protein_coding protein_coding 9.249188 6.984093 12.26162 0.3938195 0.1678555 0.8111173 5.0925966 1.8629610 10.3116859 0.9885761 0.47883669 2.462286 0.4749542 0.28390000 0.84200000 0.5581 2.162037e-01 2.266693e-07 FALSE TRUE
MSTRG.8505.1 ENSG00000132970 HEK293_OSMI2_2hA HEK293_TMG_2hB WASF3 protein_coding   9.249188 6.984093 12.26162 0.3938195 0.1678555 0.8111173 0.6158832 0.3868821 1.1386179 0.3217556 0.49528735 1.533117 0.0697625 0.05116667 0.09226667 0.0411 6.651760e-01 2.266693e-07 TRUE TRUE
MSTRG.8505.7 ENSG00000132970 HEK293_OSMI2_2hA HEK293_TMG_2hB WASF3 protein_coding   9.249188 6.984093 12.26162 0.3938195 0.1678555 0.8111173 3.1725644 4.7016671 0.4501366 1.1026632 0.09311438 -3.356104 0.4189667 0.66013333 0.03663333 -0.6235 2.266693e-07 2.266693e-07 FALSE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000132970 E001 1.3169359 0.2226550956 3.444881e-02 8.062044e-02 13 26557676 26557682 7 + 0.447 0.000 -11.216
ENSG00000132970 E002 4.0378031 0.0778394996 8.240960e-04 3.345459e-03 13 26557683 26557702 20 + 0.792 0.185 -3.289
ENSG00000132970 E003 4.9277537 0.0399499578 4.248680e-05 2.434222e-04 13 26557703 26557705 3 + 0.869 0.185 -3.595
ENSG00000132970 E004 5.0729264 0.0451851729 4.163157e-05 2.389486e-04 13 26557706 26557713 8 + 0.881 0.185 -3.638
ENSG00000132970 E005 7.6782582 0.0023438875 3.554517e-08 3.943503e-07 13 26557714 26557730 17 + 1.034 0.313 -3.215
ENSG00000132970 E006 22.6112164 0.0302648389 2.157254e-05 1.327029e-04 13 26557731 26557819 89 + 1.425 1.066 -1.269
ENSG00000132970 E007 0.3268771 0.0293275249 6.739689e-01   13 26593608 26593722 115 + 0.079 0.184 1.400
ENSG00000132970 E008 0.7427016 0.0156455526 6.992168e-02 1.432472e-01 13 26610593 26610646 54 + 0.302 0.000 -10.371
ENSG00000132970 E009 42.3113683 0.0267745866 8.276941e-04 3.358410e-03 13 26612961 26613058 98 + 1.653 1.484 -0.578
ENSG00000132970 E010 0.0000000       13 26641946 26642103 158 +      
ENSG00000132970 E011 65.9648683 0.0392700846 1.752308e-02 4.609153e-02 13 26642261 26642403 143 + 1.818 1.741 -0.262
ENSG00000132970 E012 77.9675934 0.0075192129 3.533981e-05 2.063944e-04 13 26665028 26665162 135 + 1.890 1.814 -0.256
ENSG00000132970 E013 88.9522455 0.0004368719 1.828510e-08 2.147720e-07 13 26667517 26667670 154 + 1.938 1.884 -0.183
ENSG00000132970 E014 72.4545669 0.0104852694 2.561366e-04 1.200829e-03 13 26671872 26671989 118 + 1.856 1.780 -0.258
ENSG00000132970 E015 82.6338145 0.0060921500 3.716896e-04 1.664905e-03 13 26676549 26676724 176 + 1.898 1.870 -0.094
ENSG00000132970 E016 7.4986792 0.0024972950 5.377592e-04 2.301210e-03 13 26680035 26680201 167 + 0.983 0.672 -1.221
ENSG00000132970 E017 66.1574908 0.0102202700 5.838583e-02 1.238622e-01 13 26681054 26681320 267 + 1.778 1.820 0.144
ENSG00000132970 E018 35.2936850 0.0288549986 6.143524e-01 7.328494e-01 13 26682607 26682839 233 + 1.485 1.599 0.391
ENSG00000132970 E019 34.5259447 0.0007279925 5.270594e-02 1.139682e-01 13 26682840 26682974 135 + 1.501 1.548 0.164
ENSG00000132970 E020 904.0574140 0.0110446794 9.458443e-12 1.979533e-10 13 26685688 26688948 3261 + 2.797 3.116 1.061