ENSG00000132964

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000381527 ENSG00000132964 HEK293_OSMI2_2hA HEK293_TMG_2hB CDK8 protein_coding protein_coding 9.267442 4.834268 14.11738 0.8684003 0.5555443 1.544143 4.9069825 1.2106444 8.069562 0.04486886 0.6224580 2.7266341 0.46345417 0.2682000 0.57646667 0.308266667 0.01086360 0.0108636 FALSE TRUE
ENST00000477277 ENSG00000132964 HEK293_OSMI2_2hA HEK293_TMG_2hB CDK8 protein_coding retained_intron 9.267442 4.834268 14.11738 0.8684003 0.5555443 1.544143 1.3393665 2.3159871 2.059572 0.79811397 0.9579165 -0.1685106 0.23049167 0.4482667 0.14203333 -0.306233333 0.10081535 0.0108636 FALSE TRUE
ENST00000536792 ENSG00000132964 HEK293_OSMI2_2hA HEK293_TMG_2hB CDK8 protein_coding nonsense_mediated_decay 9.267442 4.834268 14.11738 0.8684003 0.5555443 1.544143 0.5468766 0.1467337 0.680050 0.07668604 0.2003242 2.1383858 0.05032083 0.0383000 0.04746667 0.009166667 0.80088937 0.0108636 FALSE TRUE
MSTRG.8503.3 ENSG00000132964 HEK293_OSMI2_2hA HEK293_TMG_2hB CDK8 protein_coding   9.267442 4.834268 14.11738 0.8684003 0.5555443 1.544143 0.3023676 0.8979239 0.000000 0.46078552 0.0000000 -6.5044995 0.06272500 0.1950333 0.00000000 -0.195033333 0.06818794 0.0108636 FALSE TRUE
MSTRG.8503.4 ENSG00000132964 HEK293_OSMI2_2hA HEK293_TMG_2hB CDK8 protein_coding   9.267442 4.834268 14.11738 0.8684003 0.5555443 1.544143 1.9521269 0.1661135 2.917125 0.16611351 0.4611144 4.0549069 0.16769583 0.0350000 0.20520000 0.170200000 0.09095272 0.0108636 FALSE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000132964 E001 2.6324122 0.0068521997 5.269879e-01 0.6601618033 13 26254104 26254128 25 + 0.538 0.450 -0.431
ENSG00000132964 E002 18.3746777 0.0270323105 2.466868e-01 0.3820639842 13 26254129 26254245 117 + 1.258 1.140 -0.418
ENSG00000132964 E003 28.3532641 0.0130651072 8.133473e-01 0.8816512932 13 26254246 26254534 289 + 1.412 1.421 0.034
ENSG00000132964 E004 30.8393655 0.0056125072 5.249838e-01 0.6584087971 13 26254535 26254769 235 + 1.454 1.441 -0.047
ENSG00000132964 E005 0.7427016 0.0153787590 2.111140e-01 0.3398330802 13 26324772 26324821 50 + 0.273 0.000 -10.506
ENSG00000132964 E006 41.6676154 0.0007312309 6.945793e-02 0.1424614791 13 26337567 26337642 76 + 1.594 1.520 -0.256
ENSG00000132964 E007 59.4178903 0.0004155810 3.340163e-03 0.0112633057 13 26349072 26349182 111 + 1.754 1.637 -0.397
ENSG00000132964 E008 69.3605053 0.0003823258 4.117417e-05 0.0002366603 13 26353740 26353880 141 + 1.829 1.661 -0.569
ENSG00000132964 E009 41.9270388 0.0036528508 1.813700e-02 0.0474245016 13 26382814 26382871 58 + 1.610 1.486 -0.423
ENSG00000132964 E010 63.1405063 0.0032243485 1.629396e-01 0.2789786132 13 26385211 26385342 132 + 1.765 1.728 -0.124
ENSG00000132964 E011 63.7918701 0.0004380099 2.283935e-01 0.3605044762 13 26393367 26393510 144 + 1.764 1.744 -0.066
ENSG00000132964 E012 0.3030308 0.3845921094 1.000000e+00   13 26396217 26396284 68 + 0.131 0.000 -9.191
ENSG00000132964 E013 44.8023830 0.0004983986 6.908280e-01 0.7924061581 13 26396285 26396354 70 + 1.602 1.619 0.056
ENSG00000132964 E014 49.6795426 0.0005410257 7.049867e-01 0.8034265683 13 26397153 26397225 73 + 1.633 1.694 0.206
ENSG00000132964 E015 1.9143630 0.1507987091 5.116266e-01 0.6465980981 13 26400073 26400452 380 + 0.465 0.341 -0.689
ENSG00000132964 E016 62.0007147 0.0003815466 9.248460e-01 0.9566646372 13 26400453 26400550 98 + 1.735 1.780 0.153
ENSG00000132964 E017 62.4028692 0.0004356710 3.949705e-01 0.5388087000 13 26401269 26401347 79 + 1.728 1.810 0.276
ENSG00000132964 E018 27.2426258 0.0007131218 7.674734e-01 0.8497047383 13 26401466 26401468 3 + 1.394 1.412 0.061
ENSG00000132964 E019 90.9053369 0.0003699140 9.548081e-01 0.9756759019 13 26401469 26401624 156 + 1.897 1.939 0.143
ENSG00000132964 E020 320.8844931 0.0107279163 1.741817e-03 0.0064140170 13 26403956 26405238 1283 + 2.402 2.568 0.551