ENSG00000132953

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000255305 ENSG00000132953 HEK293_OSMI2_2hA HEK293_TMG_2hB XPO4 protein_coding protein_coding 6.463726 1.679838 10.54939 0.2161574 0.2000732 2.643569 3.4502472 1.2936796 5.494246 0.17159335 0.03780476 2.077956 0.61322917 0.7694000 0.5212000 -0.2482000 2.508746e-05 1.221736e-10 FALSE TRUE
MSTRG.8429.4 ENSG00000132953 HEK293_OSMI2_2hA HEK293_TMG_2hB XPO4 protein_coding   6.463726 1.679838 10.54939 0.2161574 0.2000732 2.643569 0.7469052 0.3739087 1.095116 0.05198631 0.14775453 1.525363 0.17073333 0.2221667 0.1035000 -0.1186667 3.021578e-02 1.221736e-10 FALSE TRUE
MSTRG.8429.5 ENSG00000132953 HEK293_OSMI2_2hA HEK293_TMG_2hB XPO4 protein_coding   6.463726 1.679838 10.54939 0.2161574 0.2000732 2.643569 1.2598619 0.0000000 2.403746 0.00000000 0.17757207 7.915130 0.11490000 0.0000000 0.2280000 0.2280000 1.221736e-10 1.221736e-10 FALSE TRUE
MSTRG.8429.6 ENSG00000132953 HEK293_OSMI2_2hA HEK293_TMG_2hB XPO4 protein_coding   6.463726 1.679838 10.54939 0.2161574 0.2000732 2.643569 0.9927007 0.0000000 1.556279 0.00000000 0.24877027 7.291197 0.09000417 0.0000000 0.1473667 0.1473667 4.895225e-06 1.221736e-10 FALSE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000132953 E001 0.8867404 0.1003493238 1.072452e-01 2.014569e-01 13 20777329 20777406 78 - 0.148 0.519 2.497
ENSG00000132953 E002 1.3370202 0.0146312193 2.601105e-01 3.975888e-01 13 20777407 20777448 42 - 0.259 0.523 1.516
ENSG00000132953 E003 6.0006626 0.0048420418 7.149002e-03 2.161093e-02 13 20777449 20777791 343 - 0.643 1.075 1.680
ENSG00000132953 E004 425.8294750 0.0055953880 6.934227e-23 6.076173e-21 13 20777792 20781689 3898 - 2.415 2.804 1.297
ENSG00000132953 E005 312.9600307 0.0001691829 2.435356e-08 2.791619e-07 13 20781690 20783919 2230 - 2.389 2.327 -0.209
ENSG00000132953 E006 40.0274160 0.0150689789 9.921677e-01 9.992143e-01 13 20786965 20787057 93 - 1.485 1.563 0.267
ENSG00000132953 E007 43.3175053 0.0162282968 5.869633e-01 7.103833e-01 13 20787481 20787598 118 - 1.507 1.637 0.443
ENSG00000132953 E008 36.7207228 0.0025662911 5.906369e-01 7.134711e-01 13 20788486 20788616 131 - 1.439 1.557 0.402
ENSG00000132953 E009 45.3101805 0.0005412455 8.905613e-01 9.341042e-01 13 20790462 20790580 119 - 1.532 1.618 0.295
ENSG00000132953 E010 59.1888946 0.0004518200 9.587284e-02 1.841978e-01 13 20796076 20796256 181 - 1.626 1.796 0.575
ENSG00000132953 E011 81.9383589 0.0003226835 7.259241e-01 8.190787e-01 13 20796764 20797057 294 - 1.784 1.880 0.323
ENSG00000132953 E012 68.4434199 0.0004088549 1.149663e-01 2.127889e-01 13 20799165 20799339 175 - 1.728 1.719 -0.029
ENSG00000132953 E013 65.4455000 0.0004093817 1.101018e-02 3.118602e-02 13 20800156 20800325 170 - 1.719 1.650 -0.236
ENSG00000132953 E014 55.1112715 0.0004430055 3.028786e-02 7.253305e-02 13 20800831 20800990 160 - 1.643 1.584 -0.199
ENSG00000132953 E015 51.3570997 0.0011440989 4.714411e-03 1.513627e-02 13 20807457 20807634 178 - 1.622 1.507 -0.393
ENSG00000132953 E016 47.3469683 0.0006493179 1.300171e-03 4.977137e-03 13 20808436 20808581 146 - 1.592 1.439 -0.523
ENSG00000132953 E017 47.9621139 0.0009038106 4.223405e-02 9.514942e-02 13 20809083 20809225 143 - 1.587 1.528 -0.203
ENSG00000132953 E018 58.1211789 0.0029609458 4.846464e-03 1.550428e-02 13 20809791 20809967 177 - 1.673 1.557 -0.395
ENSG00000132953 E019 57.8168644 0.0100043499 7.583761e-03 2.272933e-02 13 20821704 20821878 175 - 1.674 1.528 -0.500
ENSG00000132953 E020 48.5489551 0.0005021560 4.174180e-03 1.363330e-02 13 20822132 20822289 158 - 1.596 1.475 -0.416
ENSG00000132953 E021 43.7628760 0.0005705997 5.773768e-03 1.801837e-02 13 20827067 20827179 113 - 1.552 1.427 -0.430
ENSG00000132953 E022 46.3861801 0.0059835626 3.135743e-01 4.561844e-01 13 20842895 20843048 154 - 1.560 1.557 -0.011
ENSG00000132953 E023 41.9718891 0.0005715081 4.132258e-03 1.351222e-02 13 20843770 20843886 117 - 1.539 1.401 -0.475
ENSG00000132953 E024 19.8812182 0.0012158695 3.155896e-02 7.499311e-02 13 20855627 20855633 7 - 1.231 1.076 -0.556
ENSG00000132953 E025 43.5619250 0.0005385200 2.337158e-05 1.426580e-04 13 20855634 20855765 132 - 1.567 1.311 -0.881
ENSG00000132953 E026 46.6351554 0.0006047024 6.119988e-06 4.271815e-05 13 20862717 20862858 142 - 1.598 1.328 -0.930
ENSG00000132953 E027 6.9326887 0.0465948718 7.202069e-02 1.466934e-01 13 20862969 20863123 155 - 0.835 0.525 -1.314
ENSG00000132953 E028 0.4355181 0.6495111767 1.000000e+00 1.000000e+00 13 20863124 20863139 16 - 0.148 0.000 -8.783
ENSG00000132953 E029 0.0000000       13 20868413 20868595 183 -      
ENSG00000132953 E030 29.9406347 0.0006266670 1.348598e-04 6.805750e-04 13 20868596 20868701 106 - 1.415 1.129 -1.004
ENSG00000132953 E031 1.4854032 0.0089393650 1.066684e-01 2.006336e-01 13 20898896 20898951 56 - 0.373 0.000 -11.998
ENSG00000132953 E032 21.1265031 0.0008609145 3.315056e-04 1.506784e-03 13 20902670 20903048 379 - 1.277 0.944 -1.203