ENSG00000132952

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000255304 ENSG00000132952 HEK293_OSMI2_2hA HEK293_TMG_2hB USPL1 protein_coding protein_coding 8.49885 2.276498 15.0378 0.1485781 1.333042 2.718341 5.9512240 0.9385513 10.6929923 0.3824409 0.5496780 3.4961446 0.65000417 0.4373000 0.7168 0.2795000 4.471489e-01 2.073504e-10 FALSE TRUE
ENST00000465952 ENSG00000132952 HEK293_OSMI2_2hA HEK293_TMG_2hB USPL1 protein_coding processed_transcript 8.49885 2.276498 15.0378 0.1485781 1.333042 2.718341 1.5816099 0.0000000 3.4296839 0.0000000 0.6551600 8.4261322 0.10662083 0.0000000 0.2236 0.2236000 2.073504e-10 2.073504e-10 FALSE FALSE
ENST00000614860 ENSG00000132952 HEK293_OSMI2_2hA HEK293_TMG_2hB USPL1 protein_coding protein_coding 8.49885 2.276498 15.0378 0.1485781 1.333042 2.718341 0.3800885 0.7131227 0.2468453 0.1997169 0.1236246 -1.4933409 0.12807500 0.3132333 0.0151 -0.2981333 1.325393e-03 2.073504e-10 FALSE TRUE
MSTRG.8554.3 ENSG00000132952 HEK293_OSMI2_2hA HEK293_TMG_2hB USPL1 protein_coding   8.49885 2.276498 15.0378 0.1485781 1.333042 2.718341 0.4954428 0.5887382 0.6682755 0.5321343 0.3929362 0.1799461 0.07606667 0.2351667 0.0445 -0.1906667 8.793627e-01 2.073504e-10 FALSE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000132952 E001 1.832993 0.0749205041 5.093487e-01 6.446768e-01 13 30617693 30617916 224 + 0.353 0.286 -0.434
ENSG00000132952 E002 1.839336 0.0089455421 4.747916e-01 6.135973e-01 13 30617917 30617919 3 + 0.354 0.285 -0.443
ENSG00000132952 E003 6.810077 0.0250222113 9.857155e-02 1.883133e-01 13 30617920 30617936 17 + 0.764 0.578 -0.785
ENSG00000132952 E004 31.186404 0.0057992945 4.036427e-04 1.788620e-03 13 30617937 30618056 120 + 1.363 1.145 -0.767
ENSG00000132952 E005 34.583672 0.0171141072 9.147551e-02 1.774390e-01 13 30621073 30621221 149 + 1.386 1.367 -0.065
ENSG00000132952 E006 18.316088 0.0132870748 7.170843e-01 8.125746e-01 13 30621222 30621239 18 + 1.095 1.290 0.694
ENSG00000132952 E007 59.649864 0.0004295136 2.364990e-04 1.118619e-03 13 30621764 30621892 129 + 1.623 1.535 -0.299
ENSG00000132952 E008 163.320449 0.0008014750 4.083846e-10 6.503088e-09 13 30630835 30631474 640 + 2.055 1.958 -0.324
ENSG00000132952 E009 53.456898 0.0017505305 2.424847e-03 8.536599e-03 13 30637744 30637857 114 + 1.573 1.512 -0.209
ENSG00000132952 E010 54.176016 0.0017851156 5.280247e-03 1.669465e-02 13 30642628 30642757 130 + 1.576 1.536 -0.136
ENSG00000132952 E011 49.999495 0.0004980876 4.096341e-04 1.811214e-03 13 30646932 30647057 126 + 1.548 1.445 -0.355
ENSG00000132952 E012 56.143134 0.0030535831 3.233759e-03 1.095481e-02 13 30653148 30653305 158 + 1.593 1.536 -0.193
ENSG00000132952 E013 561.241333 0.0121865338 4.220185e-10 6.699358e-09 13 30657474 30660770 3297 + 2.518 2.848 1.098