ENSG00000132823

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000255174 ENSG00000132823 HEK293_OSMI2_2hA HEK293_TMG_2hB OSER1 protein_coding protein_coding 22.75547 15.95984 28.48824 4.839555 0.1028376 0.8355233 4.715247 4.624894 5.852551 1.0051991 0.7303393 0.3389923 0.2152833 0.3046667 0.2053667 -0.09930000 0.1641778841 6.70821e-05 FALSE TRUE
ENST00000372970 ENSG00000132823 HEK293_OSMI2_2hA HEK293_TMG_2hB OSER1 protein_coding protein_coding 22.75547 15.95984 28.48824 4.839555 0.1028376 0.8355233 6.654083 4.070238 6.862304 1.3259379 0.7116917 0.7521404 0.3049250 0.2571000 0.2409000 -0.01620000 0.9181191970 6.70821e-05 FALSE TRUE
MSTRG.20734.3 ENSG00000132823 HEK293_OSMI2_2hA HEK293_TMG_2hB OSER1 protein_coding   22.75547 15.95984 28.48824 4.839555 0.1028376 0.8355233 5.351252 4.583102 6.061674 1.8069658 0.7100248 0.4026254 0.2476000 0.2695000 0.2126667 -0.05683333 0.6606559023 6.70821e-05 FALSE TRUE
MSTRG.20734.4 ENSG00000132823 HEK293_OSMI2_2hA HEK293_TMG_2hB OSER1 protein_coding   22.75547 15.95984 28.48824 4.839555 0.1028376 0.8355233 6.034891 2.681604 9.711716 0.7913012 0.8411683 1.8527449 0.2322042 0.1688000 0.3410667 0.17226667 0.0000670821 6.70821e-05 FALSE FALSE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000132823 E001 43.890043 0.0037505634 0.003149880 0.01070684 20 44195939 44196495 557 - 1.571 1.742 0.580
ENSG00000132823 E002 81.740580 0.0003296556 0.936834812 0.96429042 20 44196496 44196850 355 - 1.924 1.934 0.034
ENSG00000132823 E003 188.000961 0.0023923574 0.007713406 0.02305911 20 44196851 44197155 305 - 2.243 2.331 0.294
ENSG00000132823 E004 509.605083 0.0002806443 0.910442518 0.94703621 20 44197156 44197739 584 - 2.718 2.722 0.014
ENSG00000132823 E005 174.858213 0.0008060855 0.512888673 0.64776814 20 44202961 44203074 114 - 2.261 2.246 -0.051
ENSG00000132823 E006 1.215694 0.0109434030 0.709734968 0.80703063 20 44206869 44206880 12 - 0.328 0.398 0.417
ENSG00000132823 E007 165.085719 0.0002813604 0.017397390 0.04581752 20 44206881 44206998 118 - 2.256 2.200 -0.189
ENSG00000132823 E008 4.839209 0.0033734756 0.012549534 0.03485058 20 44207285 44207319 35 - 0.905 0.560 -1.417
ENSG00000132823 E009 3.873070 0.0627794153 0.517423239 0.65181352 20 44207554 44207593 40 - 0.740 0.606 -0.569
ENSG00000132823 E010 4.100546 0.0068067235 0.069091547 0.14187128 20 44210012 44210076 65 - 0.821 0.560 -1.096
ENSG00000132823 E011 91.692261 0.0003287377 0.040296926 0.09158357 20 44210696 44210884 189 - 2.009 1.942 -0.224