ENSG00000132819

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000344785 ENSG00000132819 HEK293_OSMI2_2hA HEK293_TMG_2hB RBM38 protein_coding nonsense_mediated_decay 50.85058 86.68832 40.56833 5.092317 0.7905962 -1.095295 7.045482 23.53765 0.7882881 1.891078 0.443046 -4.8825297 0.1283083 0.2761667 0.0193000 -0.2568667 0.03410722 0.02076181 FALSE FALSE
ENST00000356208 ENSG00000132819 HEK293_OSMI2_2hA HEK293_TMG_2hB RBM38 protein_coding protein_coding 50.85058 86.68832 40.56833 5.092317 0.7905962 -1.095295 21.153557 26.71798 20.2623073 4.130776 1.300596 -0.3988403 0.3999083 0.3078000 0.5000333 0.1922333 0.02671848 0.02076181 FALSE TRUE
MSTRG.20898.5 ENSG00000132819 HEK293_OSMI2_2hA HEK293_TMG_2hB RBM38 protein_coding   50.85058 86.68832 40.56833 5.092317 0.7905962 -1.095295 17.855613 33.24467 11.0470315 5.800311 5.557202 -1.5885928 0.3535000 0.3798667 0.2726667 -0.1072000 0.84108553 0.02076181 TRUE TRUE
MSTRG.20898.6 ENSG00000132819 HEK293_OSMI2_2hA HEK293_TMG_2hB RBM38 protein_coding   50.85058 86.68832 40.56833 5.092317 0.7905962 -1.095295 2.111288 0.00000 5.1680585 0.000000 5.168058 9.0162674 0.0633250 0.0000000 0.1268333 0.1268333 0.41698408 0.02076181 TRUE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000132819 E001 3.372534 0.0792178366 1.877184e-09 2.643477e-08 20 57391396 57391427 32 + 1.097 0.000 -15.158
ENSG00000132819 E002 6.328579 0.0153343300 4.150043e-21 3.027390e-19 20 57391428 57391452 25 + 1.353 0.000 -18.604
ENSG00000132819 E003 12.369377 0.0014026913 2.120421e-36 5.689907e-34 20 57391453 57391498 46 + 1.604 0.266 -5.536
ENSG00000132819 E004 17.583716 0.0010030627 2.127778e-52 1.471172e-49 20 57391499 57391545 47 + 1.758 0.313 -5.736
ENSG00000132819 E005 33.138105 0.0090516322 4.569368e-19 2.622397e-17 20 57391546 57391593 48 + 1.850 1.189 -2.271
ENSG00000132819 E006 64.187090 0.0174637275 1.964273e-07 1.884848e-06 20 57391594 57391636 43 + 2.004 1.618 -1.301
ENSG00000132819 E007 346.292491 0.0028463158 7.026138e-07 6.030358e-06 20 57391637 57391795 159 + 2.581 2.439 -0.475
ENSG00000132819 E008 244.593081 0.0010525203 6.564783e-03 2.009531e-02 20 57391796 57391818 23 + 2.376 2.311 -0.217
ENSG00000132819 E009 3.425711 0.0053435048 9.609144e-04 3.824901e-03 20 57392230 57392396 167 + 0.905 0.429 -2.059
ENSG00000132819 E010 11.895734 0.0025977318 1.094003e-04 5.652839e-04 20 57392397 57392492 96 + 1.292 0.924 -1.330
ENSG00000132819 E011 490.280117 0.0031820679 5.907934e-03 1.836777e-02 20 57392654 57392777 124 + 2.680 2.612 -0.226
ENSG00000132819 E012 267.778393 0.0032965814 1.847151e-01 3.071158e-01 20 57393279 57393333 55 + 2.392 2.359 -0.108
ENSG00000132819 E013 4.414870 0.0037314369 5.425221e-01 6.732559e-01 20 57399928 57399968 41 + 0.742 0.662 -0.333
ENSG00000132819 E014 1481.663403 0.0014768787 1.518422e-01 2.641753e-01 20 57407543 57408159 617 + 3.118 3.109 -0.029
ENSG00000132819 E015 589.079611 0.0004659619 6.527981e-01 7.634508e-01 20 57408160 57408261 102 + 2.696 2.718 0.071
ENSG00000132819 E016 1895.078236 0.0009318334 1.821915e-10 3.073246e-09 20 57408262 57408913 652 + 3.141 3.243 0.339
ENSG00000132819 E017 617.443989 0.0036935293 3.946351e-05 2.277953e-04 20 57408914 57409013 100 + 2.620 2.767 0.490
ENSG00000132819 E018 378.356967 0.0099461587 8.720174e-03 2.558758e-02 20 57409014 57409224 211 + 2.404 2.556 0.505
ENSG00000132819 E019 57.640210 0.0599224034 9.287164e-03 2.700203e-02 20 57409225 57409333 109 + 1.407 1.782 1.278