ENSG00000132749

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000255087 ENSG00000132749 HEK293_OSMI2_2hA HEK293_TMG_2hB TESMIN protein_coding protein_coding 4.913497 7.492333 3.622531 0.7154925 0.3465728 -1.046364 0.3151918 0.2299891 0.5081312 0.02007941 0.02209008 1.1103488 0.07960833 0.0310000 0.14180000 0.11080000 3.250385e-06 3.250385e-06 FALSE TRUE
ENST00000432435 ENSG00000132749 HEK293_OSMI2_2hA HEK293_TMG_2hB TESMIN protein_coding processed_transcript 4.913497 7.492333 3.622531 0.7154925 0.3465728 -1.046364 1.0820301 2.2295307 0.3481151 0.66188352 0.18559043 -2.6447010 0.17460833 0.2865667 0.08806667 -0.19850000 2.612206e-01 3.250385e-06 FALSE FALSE
ENST00000443940 ENSG00000132749 HEK293_OSMI2_2hA HEK293_TMG_2hB TESMIN protein_coding protein_coding 4.913497 7.492333 3.622531 0.7154925 0.3465728 -1.046364 0.7850499 0.9086214 0.5179374 0.04313871 0.06892504 -0.7991035 0.19123750 0.1241000 0.14216667 0.01806667 8.298825e-01 3.250385e-06 FALSE TRUE
ENST00000538944 ENSG00000132749 HEK293_OSMI2_2hA HEK293_TMG_2hB TESMIN protein_coding retained_intron 4.913497 7.492333 3.622531 0.7154925 0.3465728 -1.046364 1.8508189 2.6342310 1.4651010 0.26228982 0.23592789 -0.8420345 0.36767917 0.3629000 0.39980000 0.03690000 8.674463e-01 3.250385e-06 FALSE FALSE
ENST00000544963 ENSG00000132749 HEK293_OSMI2_2hA HEK293_TMG_2hB TESMIN protein_coding protein_coding 4.913497 7.492333 3.622531 0.7154925 0.3465728 -1.046364 0.2637907 0.4248146 0.0000000 0.42481460 0.00000000 -5.4423285 0.04942500 0.0594000 0.00000000 -0.05940000 9.145608e-01 3.250385e-06 FALSE TRUE
MSTRG.5858.7 ENSG00000132749 HEK293_OSMI2_2hA HEK293_TMG_2hB TESMIN protein_coding   4.913497 7.492333 3.622531 0.7154925 0.3465728 -1.046364 0.3587238 0.6096787 0.5160535 0.16635202 0.16795330 -0.2363110 0.08382500 0.0813000 0.15390000 0.07260000 6.371532e-01 3.250385e-06 FALSE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000132749 E001 0.5180316 0.0235393063 2.170261e-01 0.346806371 11 68707440 68707441 2 - 0.000 0.242 9.480
ENSG00000132749 E002 1.2618791 0.0109195896 2.567023e-02 0.063258000 11 68707442 68707558 117 - 0.550 0.176 -2.352
ENSG00000132749 E003 3.7665058 0.0043880406 1.571723e-02 0.042100842 11 68707559 68707854 296 - 0.842 0.511 -1.404
ENSG00000132749 E004 3.5137257 0.0044338032 8.665599e-03 0.025447540 11 68707855 68708365 511 - 0.842 0.476 -1.574
ENSG00000132749 E005 2.6344886 0.0061554214 9.561313e-05 0.000501429 11 68708366 68708500 135 - 0.842 0.243 -2.989
ENSG00000132749 E006 0.0000000       11 68710511 68710873 363 -      
ENSG00000132749 E007 4.5759426 0.0035653076 1.269372e-03 0.004875336 11 68710874 68711049 176 - 0.957 0.543 -1.693
ENSG00000132749 E008 2.8001940 0.0057672391 6.508529e-01 0.761848506 11 68713270 68713407 138 - 0.599 0.543 -0.253
ENSG00000132749 E009 2.9495220 0.0062541571 1.681528e-02 0.044525873 11 68715837 68715939 103 - 0.785 0.439 -1.546
ENSG00000132749 E010 0.0000000       11 68732587 68732914 328 -      
ENSG00000132749 E011 0.0000000       11 68733654 68733725 72 -      
ENSG00000132749 E012 37.2053591 0.0208190596 4.534584e-01 0.594195033 11 68736132 68737327 1196 - 1.499 1.582 0.282
ENSG00000132749 E013 18.6110087 0.0010050337 3.288377e-01 0.472331760 11 68737328 68737858 531 - 1.303 1.248 -0.193
ENSG00000132749 E014 3.7848616 0.0050654254 3.981930e-01 0.541932599 11 68737859 68737885 27 - 0.550 0.698 0.649
ENSG00000132749 E015 8.6972004 0.0039018026 7.862277e-01 0.863097340 11 68737886 68738461 576 - 0.915 0.966 0.192
ENSG00000132749 E016 6.4203761 0.0031169136 4.343024e-01 0.576684343 11 68738462 68738699 238 - 0.754 0.874 0.475
ENSG00000132749 E017 12.6903770 0.0018117182 8.995510e-02 0.175070616 11 68738700 68738788 89 - 1.188 1.051 -0.496
ENSG00000132749 E018 0.2987644 0.0271999945 8.485433e-02   11 68738789 68738798 10 - 0.267 0.000 -13.198
ENSG00000132749 E019 9.2122303 0.0020093305 8.190583e-02 0.162550674 11 68742318 68742394 77 - 1.081 0.916 -0.609
ENSG00000132749 E020 7.7001520 0.0415835781 7.376224e-01 0.827732903 11 68744873 68744990 118 - 0.937 0.902 -0.134
ENSG00000132749 E021 29.9879041 0.0008021709 7.763481e-01 0.855977922 11 68744991 68745111 121 - 1.436 1.476 0.137
ENSG00000132749 E022 4.1255689 0.0039549628 2.583867e-01 0.395630573 11 68747146 68747207 62 - 0.550 0.740 0.818
ENSG00000132749 E023 45.0359896 0.0053636524 7.774269e-02 0.155841338 11 68747208 68747366 159 - 1.549 1.677 0.437
ENSG00000132749 E024 58.2567784 0.0214605523 3.092808e-02 0.073776204 11 68750190 68750699 510 - 1.607 1.796 0.640
ENSG00000132749 E025 7.9815578 0.0323132122 7.135525e-01 0.810018820 11 68751420 68751534 115 - 0.936 0.908 -0.108