ENSG00000132664

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000377603 ENSG00000132664 HEK293_OSMI2_2hA HEK293_TMG_2hB POLR3F protein_coding protein_coding 11.52426 6.659567 18.31652 0.4630995 0.6003743 1.458268 10.0594238 4.981070 16.13604 0.5378388 0.5523692 1.693759 0.8521958 0.7443667 0.8810333 0.1366667 6.265273e-03 9.798901e-21 FALSE TRUE
ENST00000497333 ENSG00000132664 HEK293_OSMI2_2hA HEK293_TMG_2hB POLR3F protein_coding processed_transcript 11.52426 6.659567 18.31652 0.4630995 0.6003743 1.458268 0.3288357 1.252914 0.00000 0.2104414 0.0000000 -6.980613 0.0460625 0.1889333 0.0000000 -0.1889333 9.798901e-21 9.798901e-21   FALSE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000132664 E001 1.628497 0.0092696508 7.614623e-01 8.453270e-01 20 18467389 18467389 1 + 0.354 0.442 0.485
ENSG00000132664 E002 1.476982 0.0098878265 6.137477e-01 7.323907e-01 20 18467390 18467394 5 + 0.318 0.442 0.708
ENSG00000132664 E003 22.939191 0.0008513460 2.228041e-03 7.930255e-03 20 18467395 18467430 36 + 1.378 1.162 -0.759
ENSG00000132664 E004 39.095305 0.0005491252 3.521529e-03 1.178865e-02 20 18467431 18467447 17 + 1.587 1.448 -0.477
ENSG00000132664 E005 63.716343 0.0004431235 3.206002e-03 1.087349e-02 20 18467448 18467568 121 + 1.784 1.687 -0.329
ENSG00000132664 E006 1.468451 0.0380426462 3.427821e-02 8.029319e-02 20 18467569 18467671 103 + 0.447 0.000 -11.501
ENSG00000132664 E007 58.337110 0.0006362986 1.510225e-04 7.515894e-04 20 18468944 18469061 118 + 1.760 1.606 -0.522
ENSG00000132664 E008 3.539839 0.0399438967 7.843462e-01 8.616799e-01 20 18469062 18469368 307 + 0.609 0.598 -0.045
ENSG00000132664 E009 48.457288 0.0005577214 6.218711e-03 1.919037e-02 20 18472842 18472909 68 + 1.668 1.560 -0.369
ENSG00000132664 E010 0.294498 0.3676203058 8.331848e-01   20 18472910 18473011 102 + 0.134 0.000 -9.239
ENSG00000132664 E011 58.901487 0.0004632082 3.296036e-01 4.731274e-01 20 18473391 18473458 68 + 1.725 1.727 0.008
ENSG00000132664 E012 74.252824 0.0021821434 4.094009e-02 9.276931e-02 20 18475075 18475187 113 + 1.838 1.786 -0.178
ENSG00000132664 E013 87.313895 0.0008692827 1.915822e-03 6.964742e-03 20 18480038 18480181 144 + 1.915 1.829 -0.289
ENSG00000132664 E014 88.581299 0.0005548840 1.389073e-02 3.797833e-02 20 18480402 18480509 108 + 1.917 1.865 -0.175
ENSG00000132664 E015 42.029176 0.0005465342 7.071335e-01 8.051321e-01 20 18481619 18481622 4 + 1.577 1.606 0.100
ENSG00000132664 E016 112.094243 0.0002603005 4.825983e-03 1.544658e-02 20 18481623 18481810 188 + 2.018 1.967 -0.171
ENSG00000132664 E017 359.586550 0.0034386468 1.436564e-17 6.912694e-16 20 18483481 18484646 1166 + 2.421 2.680 0.861