ENSG00000132640

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000254977 ENSG00000132640 HEK293_OSMI2_2hA HEK293_TMG_2hB BTBD3 protein_coding protein_coding 10.31192 5.396534 15.50734 0.789849 0.4694046 1.521105 5.2862245 1.92110196 10.5773608 0.23569058 0.7486104 2.4548468 0.45800417 0.38510000 0.68040000 0.29530000 5.381714e-02 5.367358e-06 FALSE TRUE
ENST00000399006 ENSG00000132640 HEK293_OSMI2_2hA HEK293_TMG_2hB BTBD3 protein_coding protein_coding 10.31192 5.396534 15.50734 0.789849 0.4694046 1.521105 0.7640115 0.00000000 1.6244075 0.00000000 0.4778965 7.3526239 0.05407500 0.00000000 0.10643333 0.10643333 5.367358e-06 5.367358e-06 FALSE TRUE
ENST00000422390 ENSG00000132640 HEK293_OSMI2_2hA HEK293_TMG_2hB BTBD3 protein_coding protein_coding 10.31192 5.396534 15.50734 0.789849 0.4694046 1.521105 0.8859221 0.63044999 0.0000000 0.63044999 0.0000000 -6.0010140 0.06130000 0.11893333 0.00000000 -0.11893333 6.807439e-01 5.367358e-06 FALSE TRUE
ENST00000449299 ENSG00000132640 HEK293_OSMI2_2hA HEK293_TMG_2hB BTBD3 protein_coding nonsense_mediated_decay 10.31192 5.396534 15.50734 0.789849 0.4694046 1.521105 0.5580491 0.08890759 1.2425346 0.08890759 0.3904446 3.6626254 0.03892083 0.01306667 0.07920000 0.06613333 1.199559e-01 5.367358e-06 FALSE TRUE
ENST00000455911 ENSG00000132640 HEK293_OSMI2_2hA HEK293_TMG_2hB BTBD3 protein_coding protein_coding 10.31192 5.396534 15.50734 0.789849 0.4694046 1.521105 1.1550583 1.25815970 0.7769235 0.85922028 0.6347394 -0.6884412 0.12772083 0.21340000 0.04950000 -0.16390000 6.724982e-01 5.367358e-06 FALSE TRUE
ENST00000473180 ENSG00000132640 HEK293_OSMI2_2hA HEK293_TMG_2hB BTBD3 protein_coding processed_transcript 10.31192 5.396534 15.50734 0.789849 0.4694046 1.521105 0.3495759 0.74820758 0.0000000 0.44873008 0.0000000 -6.2445210 0.06055833 0.13256667 0.00000000 -0.13256667 6.154007e-02 5.367358e-06 FALSE TRUE
ENST00000473416 ENSG00000132640 HEK293_OSMI2_2hA HEK293_TMG_2hB BTBD3 protein_coding processed_transcript 10.31192 5.396534 15.50734 0.789849 0.4694046 1.521105 0.2333568 0.47371822 0.2947903 0.40608631 0.1488108 -0.6663497 0.03485000 0.08740000 0.01963333 -0.06776667 8.512399e-01 5.367358e-06 FALSE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000132640 E001 0.7406253 0.0154053067 1.655663e-01 2.824446e-01 20 11890723 11890816 94 + 0.256 0.000 -22.988
ENSG00000132640 E002 1.0393897 0.0119745063 7.573496e-02 1.526780e-01 20 11890817 11890827 11 + 0.327 0.000 -23.473
ENSG00000132640 E003 20.2770909 0.0042757261 2.315291e-03 8.198749e-03 20 11890828 11890869 42 + 1.282 1.152 -0.459
ENSG00000132640 E004 31.9046427 0.0247515753 1.507049e-02 4.064836e-02 20 11890870 11890954 85 + 1.463 1.360 -0.358
ENSG00000132640 E005 0.0000000       20 11891327 11891447 121 +      
ENSG00000132640 E006 0.4031496 0.0327573240 4.070574e-02 9.233103e-02 20 11892493 11892543 51 + 0.000 0.366 25.472
ENSG00000132640 E007 4.2978563 0.1042707546 1.252822e-01 2.275354e-01 20 11917778 11917864 87 + 0.699 0.478 -0.994
ENSG00000132640 E008 4.6374188 0.0427427154 1.317141e-01 2.365113e-01 20 11917865 11917892 28 + 0.713 0.563 -0.650
ENSG00000132640 E009 7.5234304 0.1011691594 1.047761e-01 1.977753e-01 20 11917893 11918011 119 + 0.898 0.701 -0.777
ENSG00000132640 E010 2.3993243 0.0699946337 9.508238e-01 9.732219e-01 20 11918012 11918333 322 + 0.442 0.563 0.589
ENSG00000132640 E011 84.5203601 0.0128269863 5.876236e-05 3.253076e-04 20 11918334 11918601 268 + 1.881 1.754 -0.428
ENSG00000132640 E012 1.7777231 0.0130864728 4.064172e-01 5.500631e-01 20 11918810 11918850 41 + 0.416 0.365 -0.285
ENSG00000132640 E013 1.0329452 0.0117400371 9.237203e-01 9.559509e-01 20 11918851 11918876 26 + 0.256 0.365 0.715
ENSG00000132640 E014 2.2926342 0.0066701335 9.833692e-01 9.936812e-01 20 11918877 11919085 209 + 0.442 0.561 0.578
ENSG00000132640 E015 57.7157988 0.0010319749 2.091214e-07 1.997249e-06 20 11919086 11919176 91 + 1.719 1.594 -0.425
ENSG00000132640 E016 1.1834139 0.0108647759 9.607131e-02 1.844781e-01 20 11919277 11919423 147 + 0.171 0.561 2.454
ENSG00000132640 E017 0.9932890 0.2582815227 3.775011e-02 8.687089e-02 20 11919424 11919540 117 + 0.065 0.575 4.105
ENSG00000132640 E018 1.1384617 0.1679503146 7.235764e-02 1.472705e-01 20 11919541 11919610 70 + 0.121 0.571 3.087
ENSG00000132640 E019 1.5812529 0.1196687015 1.050290e-01 1.981302e-01 20 11919611 11919717 107 + 0.215 0.639 2.388
ENSG00000132640 E020 71.3516932 0.0004006001 1.099156e-03 4.300709e-03 20 11919718 11919836 119 + 1.780 1.799 0.066
ENSG00000132640 E021 0.2966881 0.0269915155 6.756878e-01   20 11921566 11921662 97 + 0.121 0.000 -21.665
ENSG00000132640 E022 1057.7822545 0.0206259848 3.861107e-04 1.721158e-03 20 11922634 11926609 3976 + 2.885 3.113 0.756