ENSG00000132563

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000254901 ENSG00000132563 HEK293_OSMI2_2hA HEK293_TMG_2hB REEP2 protein_coding protein_coding 30.87549 58.29294 15.59513 2.25684 0.4242728 -1.901548 5.55122 13.62067 1.949808 1.384215 0.1750940 -2.798073 0.1654375 0.2325667 0.1248333 -0.10773333 0.0005133979 0.0005133979 FALSE TRUE
ENST00000378339 ENSG00000132563 HEK293_OSMI2_2hA HEK293_TMG_2hB REEP2 protein_coding protein_coding 30.87549 58.29294 15.59513 2.25684 0.4242728 -1.901548 20.26950 38.84091 11.572507 1.709806 0.6283817 -1.746001 0.6454125 0.6662000 0.7423333 0.07613333 0.3974057976 0.0005133979 FALSE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000132563 E001 7.967373 0.0129627379 1.235858e-11 2.535517e-10 5 138439057 138439086 30 + 1.347 0.551 -3.051
ENSG00000132563 E002 9.431682 0.0174824027 3.101331e-12 7.026283e-11 5 138439087 138439101 15 + 1.414 0.611 -3.017
ENSG00000132563 E003 9.471334 0.0131144477 1.892536e-11 3.766523e-10 5 138439102 138439109 8 + 1.393 0.645 -2.796
ENSG00000132563 E004 9.471334 0.0131144477 1.892536e-11 3.766523e-10 5 138439110 138439110 1 + 1.393 0.645 -2.796
ENSG00000132563 E005 36.621171 0.0048793154 7.877159e-07 6.690147e-06 5 138439111 138439167 57 + 1.702 1.384 -1.088
ENSG00000132563 E006 80.437583 0.0023884533 9.768463e-05 5.112030e-04 5 138439168 138439240 73 + 1.927 1.759 -0.567
ENSG00000132563 E007 1.291966 0.0104476428 2.675155e-01 4.058424e-01 5 138439682 138439826 145 + 0.458 0.267 -1.143
ENSG00000132563 E008 2.376883 0.2197106000 2.140991e-01 3.433601e-01 5 138440941 138441015 75 + 0.675 0.396 -1.323
ENSG00000132563 E009 157.100412 0.0002897111 4.133502e-05 2.374839e-04 5 138441016 138441088 73 + 2.171 2.061 -0.368
ENSG00000132563 E010 233.556095 0.0002174879 1.216525e-02 3.392713e-02 5 138441385 138441461 77 + 2.290 2.248 -0.142
ENSG00000132563 E011 3.391465 0.0120800100 1.078752e-01 2.023870e-01 5 138443587 138443858 272 + 0.730 0.484 -1.095
ENSG00000132563 E012 2.770920 0.0234760871 8.911705e-01 9.344896e-01 5 138444328 138444414 87 + 0.458 0.506 0.235
ENSG00000132563 E013 342.274556 0.0001681571 3.760409e-04 1.681454e-03 5 138444415 138444535 121 + 2.464 2.411 -0.177
ENSG00000132563 E014 6.340777 0.0068972338 3.301028e-01 4.737035e-01 5 138444536 138444705 170 + 0.615 0.797 0.754
ENSG00000132563 E015 349.339339 0.0001728225 6.313599e-02 1.319335e-01 5 138444754 138444867 114 + 2.442 2.428 -0.048
ENSG00000132563 E016 4.509012 0.0039744773 4.231489e-01 5.662640e-01 5 138444868 138444997 130 + 0.730 0.626 -0.438
ENSG00000132563 E017 7.101953 0.0023177928 5.829439e-01 7.071451e-01 5 138444998 138445227 230 + 0.860 0.808 -0.201
ENSG00000132563 E018 107.971256 0.0002930709 5.899870e-01 7.129551e-01 5 138445228 138445233 6 + 1.921 1.926 0.017
ENSG00000132563 E019 330.444607 0.0006807550 8.215325e-01 8.874480e-01 5 138445234 138445375 142 + 2.387 2.410 0.076
ENSG00000132563 E020 387.381926 0.0006448288 3.745821e-02 8.632289e-02 5 138445468 138445598 131 + 2.410 2.490 0.268
ENSG00000132563 E021 1436.197731 0.0013942302 6.937307e-15 2.341860e-13 5 138445683 138446969 1287 + 2.919 3.070 0.501