ENSG00000132478

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000586527 ENSG00000132478 HEK293_OSMI2_2hA HEK293_TMG_2hB UNK protein_coding nonsense_mediated_decay 27.97816 32.79899 28.64313 0.8591518 0.6586688 -0.1953987 12.659189 12.3118137 17.140101 2.04724069 0.2082348 0.4770025 0.4567542 0.37276667 0.5991667 0.22640000 8.062191e-03 1.391469e-07   FALSE
ENST00000589666 ENSG00000132478 HEK293_OSMI2_2hA HEK293_TMG_2hB UNK protein_coding protein_coding 27.97816 32.79899 28.64313 0.8591518 0.6586688 -0.1953987 10.984127 16.9285214 6.119231 1.04469537 0.1819359 -1.4665299 0.3855958 0.51816667 0.2140333 -0.30413333 1.391469e-07 1.391469e-07 FALSE TRUE
MSTRG.15080.3 ENSG00000132478 HEK293_OSMI2_2hA HEK293_TMG_2hB UNK protein_coding   27.97816 32.79899 28.64313 0.8591518 0.6586688 -0.1953987 0.744214 0.3563607 1.724473 0.06915693 0.3989636 2.2431609 0.0271500 0.01076667 0.0609000 0.05013333 1.627522e-04 1.391469e-07 FALSE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000132478 E001 0.1817044 0.0407208162 8.063784e-01   17 75784740 75784780 41 + 0.000 0.089 8.169
ENSG00000132478 E002 0.3268771 0.0301356588 6.870952e-01   17 75784781 75784805 25 + 0.166 0.089 -1.041
ENSG00000132478 E003 0.8513531 0.0187179756 2.941203e-01 4.351985e-01 17 75784806 75784817 12 + 0.380 0.163 -1.625
ENSG00000132478 E004 6.2625376 0.0026553940 6.306883e-02 1.318204e-01 17 75784818 75784841 24 + 1.014 0.725 -1.116
ENSG00000132478 E005 10.3022611 0.0234033017 3.202660e-02 7.591072e-02 17 75784842 75784848 7 + 1.227 0.901 -1.186
ENSG00000132478 E006 14.3243753 0.0064453028 8.355232e-02 1.651097e-01 17 75784849 75784854 6 + 1.304 1.074 -0.819
ENSG00000132478 E007 43.7507674 0.0005279610 1.522327e-03 5.709200e-03 17 75784855 75784861 7 + 1.767 1.527 -0.815
ENSG00000132478 E008 122.0202607 0.0039901064 5.655731e-08 6.023506e-07 17 75784862 75784984 123 + 2.242 1.937 -1.022
ENSG00000132478 E009 42.3231787 0.0299817296 5.928654e-05 3.279029e-04 17 75791760 75791770 11 + 1.865 1.416 -1.531
ENSG00000132478 E010 173.8361037 0.0275251176 2.104458e-06 1.629207e-05 17 75791771 75792019 249 + 2.470 2.018 -1.509
ENSG00000132478 E011 0.7761102 0.0414400390 6.327702e-01 7.475643e-01 17 75792020 75792165 146 + 0.166 0.227 0.552
ENSG00000132478 E012 168.6793727 0.0180823395 1.142521e-07 1.148322e-06 17 75792313 75792478 166 + 2.446 2.017 -1.435
ENSG00000132478 E013 0.7761102 0.0414400390 6.327702e-01 7.475643e-01 17 75792479 75792636 158 + 0.166 0.227 0.552
ENSG00000132478 E014 324.4709319 0.0293323010 2.427616e-04 1.144896e-03 17 75793443 75794198 756 + 2.688 2.338 -1.167
ENSG00000132478 E015 1.4779251 0.0154930704 2.560671e-01 3.930160e-01 17 75797257 75797397 141 + 0.523 0.281 -1.363
ENSG00000132478 E016 5.8259608 0.0028792887 2.692568e-01 4.077594e-01 17 75808948 75809039 92 + 0.928 0.725 -0.794
ENSG00000132478 E017 98.3486625 0.0017422842 6.630539e-02 1.372256e-01 17 75809760 75809930 171 + 2.048 1.901 -0.494
ENSG00000132478 E018 62.2693245 0.0023492729 9.045457e-03 2.639049e-02 17 75809931 75809969 39 + 1.887 1.684 -0.685
ENSG00000132478 E019 69.8869157 0.0029835529 6.743904e-02 1.390938e-01 17 75812112 75812147 36 + 1.910 1.748 -0.547
ENSG00000132478 E020 115.1460418 0.0061592914 4.302481e-01 5.728856e-01 17 75812148 75812288 141 + 2.093 1.983 -0.367
ENSG00000132478 E021 10.3114212 0.0033139351 4.394516e-03 1.424898e-02 17 75812289 75812360 72 + 1.227 0.867 -1.320
ENSG00000132478 E022 103.7586083 0.0034133544 7.010476e-01 8.003673e-01 17 75812455 75812585 131 + 2.010 1.957 -0.179
ENSG00000132478 E023 82.7674730 0.0093968532 4.653988e-01 6.051201e-01 17 75813078 75813213 136 + 1.955 1.840 -0.387
ENSG00000132478 E024 97.0428255 0.0020087909 9.272582e-01 9.582277e-01 17 75813761 75813878 118 + 1.996 1.920 -0.254
ENSG00000132478 E025 92.7138898 0.0080309758 8.348183e-01 8.965941e-01 17 75815169 75815253 85 + 1.981 1.899 -0.276
ENSG00000132478 E026 124.1441063 0.0092814780 6.658541e-01 7.734392e-01 17 75816770 75816912 143 + 2.116 2.021 -0.318
ENSG00000132478 E027 150.0905398 0.0066293665 8.915520e-01 9.347473e-01 17 75817326 75817526 201 + 2.185 2.109 -0.255
ENSG00000132478 E028 82.4143542 0.0019301036 4.688368e-01 6.081971e-01 17 75818103 75818168 66 + 1.900 1.862 -0.129
ENSG00000132478 E029 131.9224330 0.0023863295 3.791286e-02 8.718241e-02 17 75818642 75818816 175 + 2.066 2.078 0.042
ENSG00000132478 E030 81.8146113 0.0037323107 3.865160e-02 8.853672e-02 17 75819684 75819738 55 + 1.843 1.880 0.125
ENSG00000132478 E031 86.0748774 0.0018516726 3.020377e-03 1.032631e-02 17 75819739 75819785 47 + 1.843 1.908 0.220
ENSG00000132478 E032 177.6733365 0.0024886338 1.062366e-05 7.018095e-05 17 75819920 75820108 189 + 2.136 2.229 0.312
ENSG00000132478 E033 140.0455997 0.0030781113 4.934924e-03 1.574911e-02 17 75822477 75822560 84 + 2.071 2.116 0.150
ENSG00000132478 E034 155.5620521 0.0032082365 1.797725e-04 8.770210e-04 17 75822561 75822658 98 + 2.088 2.170 0.275
ENSG00000132478 E035 219.0416121 0.0001840786 2.973443e-13 7.897236e-12 17 75823265 75823522 258 + 2.206 2.328 0.408
ENSG00000132478 E036 418.2489354 0.0004451147 1.424737e-26 1.774570e-24 17 75824262 75824969 708 + 2.464 2.614 0.502
ENSG00000132478 E037 254.5310718 0.0002027788 2.213581e-41 8.337102e-39 17 75824970 75825399 430 + 2.142 2.426 0.946
ENSG00000132478 E038 154.9429328 0.0040705423 1.362302e-15 5.063448e-14 17 75825400 75825799 400 + 1.920 2.218 0.998