ENSG00000132463

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000254799 ENSG00000132463 HEK293_OSMI2_2hA HEK293_TMG_2hB GRSF1 protein_coding protein_coding 68.79908 55.04042 93.70018 4.690175 1.346186 0.7674521 3.675568 2.968114 5.920185 0.6876178 0.1919697 0.9936784 0.0543375 0.0528 0.06313333 0.010333333 6.417052e-01 1.276562e-05 FALSE  
ENST00000499044 ENSG00000132463 HEK293_OSMI2_2hA HEK293_TMG_2hB GRSF1 protein_coding protein_coding 68.79908 55.04042 93.70018 4.690175 1.346186 0.7674521 11.393346 11.801590 15.797104 1.3259113 0.8469355 0.4203699 0.1551000 0.2136 0.16846667 -0.045133333 7.857157e-02 1.276562e-05 FALSE  
ENST00000505068 ENSG00000132463 HEK293_OSMI2_2hA HEK293_TMG_2hB GRSF1 protein_coding retained_intron 68.79908 55.04042 93.70018 4.690175 1.346186 0.7674521 10.498645 6.214676 16.740905 0.8414254 0.5730768 1.4281685 0.1528917 0.1120 0.17856667 0.066566667 1.276562e-05 1.276562e-05 TRUE  
ENST00000506453 ENSG00000132463 HEK293_OSMI2_2hA HEK293_TMG_2hB GRSF1 protein_coding nonsense_mediated_decay 68.79908 55.04042 93.70018 4.690175 1.346186 0.7674521 23.181384 18.355878 28.074314 2.7824067 1.2858414 0.6127367 0.3428042 0.3302 0.29990000 -0.030300000 6.996014e-01 1.276562e-05 FALSE  
ENST00000514161 ENSG00000132463 HEK293_OSMI2_2hA HEK293_TMG_2hB GRSF1 protein_coding protein_coding 68.79908 55.04042 93.70018 4.690175 1.346186 0.7674521 19.816666 15.270590 27.127850 1.0987153 0.7097308 0.8286062 0.2914667 0.2840 0.28946667 0.005466667 9.625985e-01 1.276562e-05 FALSE  
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000132463 E001 364.3425232 0.0128252915 1.439193e-01 2.533506e-01 4 70815783 70819530 3748 - 2.594 2.521 -0.241
ENSG00000132463 E002 143.9843153 0.0089705152 4.427960e-02 9.889696e-02 4 70819531 70819757 227 - 2.105 2.226 0.405
ENSG00000132463 E003 738.8512195 0.0047363632 4.150768e-01 5.585025e-01 4 70819758 70820311 554 - 2.855 2.885 0.101
ENSG00000132463 E004 212.1312229 0.0001824253 7.372817e-02 1.494914e-01 4 70820312 70820374 63 - 2.350 2.306 -0.147
ENSG00000132463 E005 426.5537772 0.0002286762 6.856742e-02 1.409942e-01 4 70820375 70820698 324 - 2.617 2.650 0.109
ENSG00000132463 E006 244.6474232 0.0001782833 8.762780e-03 2.569111e-02 4 70820699 70820745 47 - 2.362 2.423 0.201
ENSG00000132463 E007 507.5328831 0.0001128133 2.220234e-02 5.609119e-02 4 70820746 70820861 116 - 2.691 2.728 0.122
ENSG00000132463 E008 541.5209145 0.0013646235 2.731728e-03 9.468115e-03 4 70824294 70824368 75 - 2.703 2.770 0.225
ENSG00000132463 E009 779.0291636 0.0002533534 2.002122e-05 1.241762e-04 4 70825296 70825431 136 - 2.865 2.924 0.197
ENSG00000132463 E010 110.1472499 0.0002650632 5.993113e-14 1.757822e-12 4 70825432 70826123 692 - 2.148 1.880 -0.899
ENSG00000132463 E011 621.1197474 0.0003693250 2.391716e-07 2.258403e-06 4 70826124 70826245 122 - 2.755 2.837 0.272
ENSG00000132463 E012 793.6253238 0.0027411279 6.673234e-01 7.746092e-01 4 70827852 70828036 185 - 2.898 2.899 0.005
ENSG00000132463 E013 668.1269432 0.0018101713 9.817917e-01 9.926518e-01 4 70831539 70831674 136 - 2.828 2.819 -0.028
ENSG00000132463 E014 746.5552227 0.0010874310 5.665310e-01 6.934907e-01 4 70832307 70832450 144 - 2.881 2.863 -0.057
ENSG00000132463 E015 725.9271293 0.0003084422 3.355313e-06 2.485742e-05 4 70833118 70833273 156 - 2.892 2.821 -0.236
ENSG00000132463 E016 427.1186951 0.0008496081 2.528190e-11 4.930087e-10 4 70836158 70836314 157 - 2.693 2.543 -0.497
ENSG00000132463 E017 0.6599951 0.0189989519 1.421283e-02 3.870927e-02 4 70838869 70839176 308 - 0.000 0.412 10.844
ENSG00000132463 E018 1.5123699 0.0090655956 1.754491e-01 2.952548e-01 4 70839177 70839227 51 - 0.497 0.253 -1.433
ENSG00000132463 E019 3.7570452 0.0041460913 4.586011e-02 1.017814e-01 4 70839228 70839470 243 - 0.784 0.474 -1.358
ENSG00000132463 E020 203.5129005 0.0083413800 5.427046e-07 4.767401e-06 4 70839471 70839897 427 - 2.410 2.138 -0.908